1887

Abstract

The prevalence of CTX-M-producing members of the is increasing worldwide. A novel, multiplex, real-time TaqMan PCR assay to detect and type genes is described which is an improvement on previously described techniques with respect to reduced assay time, elimination of the need for protracted post-PCR processing and the convenience of a single reaction vessel. Based on -lactam antibiogram and MIC data, 478 of 1279 isolates from clinical blood and urine culture specimens were selected and tested for extended-spectrum -lactamase (ESBL) production using phenotypic methods. The new TaqMan assay detected and typed genes in 21 of 28 ESBL-producing isolates.

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2007-01-01
2019-09-21
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