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Volume 56, Issue 4

A catalase mutant is hypersensitive to oxidative stress and suffers increased DNA damage Free

Abstract

Catalase (KatA) is known to play an important role in oxidative stress resistance in many bacterial species and a homologue exists in , a member of the enterohepatic species. Here, a mutant was constructed by insertional mutagenesis and its oxidative stress phenotype was investigated. Catalase activity was readily detected [196 units (mg protein crude cell extract)] in the wild-type, whereas the mutant strain was deficient in, but not devoid of, activity. In contrast, strains lack detectable catalase activity and wild-type generally contains higher specific activity than . Wild-type cells tolerated 6 % O for growth, whilst the mutant could not survive at this oxygen level. Even at the optimal O level, the growth of the strain was severely inhibited, which is also in contrast to strains. Wild-type cells withstood exposure to 100 mM HO but the mutant cells were killed by the same treatment. Wild-type cells suffered no significant DNA damage by HO treatment (100 mM for 6 min), whilst the same treatment resulted in severe DNA fragmentation in the mutant. Thus KatA plays an important role as an antioxidant protein.

  • Received:
  • Accepted:
  • Published Online:
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2007-04-01
2024-04-16
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A Helicobacter hepaticus catalase mutant is hypersensitive to oxidative stress and suffers increased DNA damage
J. Med. Microbiol. 56, 557 (2007); https://doi.org/10.1099/jmm.0.46891-0
/content/journal/jmm/10.1099/jmm.0.46891-0
/content/journal/jmm/10.1099/jmm.0.46891-0
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