1887

Abstract

pathogenicity island (PAI) integrity was investigated in isolates from multiple biopsies recovered from 40 patients in an attempt to determine the co-existence of a varying PAI-positive to PAI-negative ratio in a single host. Six biopsies were obtained from each patient during the same endoscopic session. PAI analysis included amplification of seven loci (, , , , , HP0527 and HP0524) and the left end of II (LEC). Absence of the island was confirmed by empty-site PCR. - RFLP and random amplified polymorphic DNA PCR were used for strain delineation. The number of biopsies with -positive culture ranged from three to six per patient and a total of 218 isolates were recovered. Mixed infection was only found in two patients. Nearly one-third of the 40 patients harboured isolates with an intact PAI in all niches, another third of the isolates were empty-site-positive in all niches, whilst the remaining third of the isolates had a disrupted PAI in all or at least one of the niches. Co-existence of variants of the same strain with different PAI genotypes was observed in one-quarter of patients. The variations in PAI genotype included co-existence of: diverse PAI deletions in different niches, variants with intact and with partially deleted islands, variants with empty-site-positive and with partially deleted PAIs, and variants with an intact PAI and with empty-site-positive. Half of the patients with different PAI genotypes harboured an intact PAI in at least one niche. Co-existence of diverse genotypes of putative virulence factors in a single host must be considered when drawing a correlation with clinical presentation.

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2007-05-01
2019-10-18
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