1887

Abstract

The occurrence of 7 of the 11 known genes that are found within the Sa genomic island of and encode the novel Ssl family of exoproteins was examined in isolates from cows (42 isolates), goats (4 isolates), sheep (1 isolate), rabbits (3 isolates) and chickens (2 isolates). Based on seven genome sequences for human strains NCTC 8325, N315, Mu50, COL, MRSA 252, MW2 and MSSA-476, and bovine strain RF122, along with the reference gene sequences from strains NCTC 6571, FRI326 and NCTC 8325, ClustalW-generated alignments were used to design PCR primers for unique regions of the genes that are present in the allelic variants of each, except for the gene for which specific primers for the and allelic variants were designed individually. The genotypes of isolates were determined using random amplified polymorphic DNA (RAPD) typing. All of the animal-associated isolates contained an locus, but there were minor variations in the number of genes present. Forty-nine of the animal isolates possessed a Sa genomic island containing the (), (), (), (), () and () genes. One bovine and one goat isolate lacked the gene. The gene was absent in one bovine isolate. The goat isolate lacking the gene was the only animal isolate that possessed the allele of the gene. PCR for the allele of the gene was inconclusive. Isolates that showed identical RAPD fingerprints had the same complement of genes, but the gene pattern was not RAPD-type specific. Southern blot hybridization showed similar gene RFLPs in isolates of the same RAPD type.

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2007-03-01
2019-11-22
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