1887

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Volume 56, Issue 5

A paradigm for the molecular identification of species in a routine diagnostic laboratory Free

Abstract

The aim of this study was to improve the identification of species in the context of a UK teaching hospital. Real-time PCR assays were established to enable the rapid differentiation between (MTB) complex and species other than (MOTT), followed by 16S rRNA gene sequencing for the speciation of MOTT. Real-time PCR assays gave comparable results to those from the reference laboratory. The implementation of these PCR assays using an improved bead extraction method has enhanced the mycobacterial diagnostic service at the Royal Free Hospital by providing a rapid means of differentiating between MTB complex and MOTT, and would be simple to implement in similar laboratories. Sequence analysis successfully identified a range of spp. representative of those encountered in the clinical setting of the authors, including complex, group, – group, and . It provides a useful tool for the identification of MOTT when clinically indicated.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): MOTT, Mycobacterium species other than tuberculosis and MTB, Mycobacterium tuberculosis
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2007-05-01
2024-04-19
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A paradigm for the molecular identification of Mycobacterium species in a routine diagnostic laboratory
J. Med. Microbiol. 56, 598 (2007); https://doi.org/10.1099/jmm.0.46855-0
/content/journal/jmm/10.1099/jmm.0.46855-0
/content/journal/jmm/10.1099/jmm.0.46855-0
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