1887

Journal of Medical Microbiology logo

Volume 56, Issue 5

Detection of antibodies to in serum and oral fluid from patients with cystic fibrosis Free

Abstract

Cystic fibrosis (CF) patients who are chronically infected with make serum antibodies to bacterial surface LPS as well as other pseudomonas antigens. This study investigated the feasibility of using oral fluid samples for the detection of pseudomonas antibodies in CF patients and compared these results with corresponding serum antibodies. Most strains of produce two forms of LPS molecule, termed A-band (described as a common antigen) and B-band (O-serotype-specific antigen), apparently bound to a common core oligosaccharide moiety. A-band LPS was demonstrated in 45 out of 49 clinical isolates of by SDS-PAGE and immunoblotting with a specific antibody. Oral fluids were collected from 17 adult CF patients, all of whom were sputum culture positive for (13 also provided serum samples), 11 primary ciliary dyskinesia (PCD) patients and 37 healthy volunteers. Antibodies to A-band LPS were detected by immunoblotting in all of the CF patients’ oral fluids but 10 of the volunteer samples gave weak reactions with immunoblotting. Six of the PCD patients gave a weak reaction with A-band antibodies and only one demonstrated antibodies to core LPS. In a quantitative ELISA, 15 of the 17 CF patients’ oral fluids were shown to contain antibodies to A-band LPS, whilst none of the volunteer samples contained antibodies to A-band LPS. All serum samples from the CF patients were positive by both methods. Thus this is a sensitive procedure for the detection of antibodies to A-band LPS of in oral fluid and serum from patients with CF.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): CF, cystic fibrosis , HP, high positive and PCD, primary ciliary dyskinesia
SGM
Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.46833-0
2007-05-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jmm/56/5/670.html?itemId=/content/journal/jmm/10.1099/jmm.0.46833-0&mimeType=html&fmt=ahah

References

  1. Arsenault T. L., Hughes D. W., MacLean D. B., Szarek W. A., Kropinski A. M. B., Lam J. S. 1991; Structural studies on the polysaccharide portion of ‘A-band’ lipopolysaccharide from a mutant (AK1401) of Pseudomonas aeruginosa strain PAO1. Can J Chem 69:1273–1280 [CrossRef]
     [Google Scholar]
  2. Botzenhart K., Puhr O. F., Doring G. 1985; Pseudomonas aeruginosa in the oral cavity: occurrence and age distribution of adult germ carriers. Zentralbl Bakteriol Mikrobiol Hyg [B] 180:471–479 (in German
    [Google Scholar]
  3. Brett M. M., Ghoneim A. T., Littlewood J. M., Losowsky M. S. 1986; Development of enzyme linked immunosorbent assay (ELISA) to detect antibodies to Pseudomonas aeruginosa cell surface antigens in sera of patients with cystic fibrosis. J Clin Pathol 39:1124–1129 [CrossRef]
     [Google Scholar]
  4. Chart H., Pearce M. C., Mellor D., Shaw D. J., Brown D. 2002; Immunoassays to detect the serum antibody response of cattle to infection with Salmonella Typhimurium definitive type 104 and following vaccination with Bovivac S. J Appl Microbiol 93:46–51 [CrossRef]
     [Google Scholar]
  5. Chart H., Perry N. T., Willshaw G. A., Cheasty T. 2003; Analysis of saliva for antibodies to the LPS of Escherichia coli O157 in patients with serum antibodies to E. coli O157 LPS. J Med Microbiol 52:569–572 [CrossRef]
     [Google Scholar]
  6. Doring G., Høiby N. 1983; Longitudinal study of immune response to Pseudomonas aeruginosa antigens in cystic fibrosis. Infect Immun 42:197–201
     [Google Scholar]
  7. Doring G., Conway S. P., Heijerman H. G. M., Hodson M. E., Hoiby N., Smyth A., Touw D. J. 2000; Antibiotic therapy against Pseudomonas aeruginosa in cystic fibrosis: a European consensus. Eur Respir J 16:749–767 [CrossRef]
     [Google Scholar]
  8. Fomsgaard A., Conrad R. S., Galanos C., Shand G. H., Høiby N. 1988; Comparative immunochemistry of lipopolysaccharides from typable and polyagglutinable Pseudomonas aeruginosa strains isolated from patients with cystic fibrosis. J Clin Microbiol 26:821–826
     [Google Scholar]
  9. Frederiksen B., Koch C., Hoiby N. 1997; Antibiotic treatment of initial colonization with Pseudomonas aeruginosa postpones chronic infection and prevents deterioration of pulmonary function in cystic fibrosis. Pediatr Pulmonol 23:330–335 [CrossRef]
     [Google Scholar]
  10. Govan J. R., Deretic V. 1996; Microbial pathogenesis in cystic fibrosis: mucoid Pseudomonas aeruginosa and Burkholderia cepacia . Microbiol Rev 60:539–574
     [Google Scholar]
  11. Grzybowski J., Trafny E. A., Wrembel-Wargocka J., Patzer J., Dzierzanowska D., Zawistowska-Marciniak I., Klos M. 1989; Amount, avidity, and specificity of antibodies to Pseudomonas aeruginosa in normal human sera. J Clin Microbiol 27:1367–1371
     [Google Scholar]
  12. Hancock R. E., Mutharia L. M., Chan L., Darveau R. P., Speert D. P., Pier G. B. 1983; Pseudomonas aeruginosa isolates from patients with cystic fibrosis: a class of serum-sensitive, nontypable strains deficient in lipopolysaccharide O side chains. Infect Immun 42:170–177
     [Google Scholar]
  13. Hatano K., Goldberg J. B., Pier G. B. 1993; Pseudomonas aeruginosa lipopolysaccharide: evidence that the O side chains and common antigens are on the same molecule. J Bacteriol 175:5117–5128
     [Google Scholar]
  14. Kocharova N. A., Knirel Y. A., Shashkov A. S., Kochetkov N. K., Pier G. B. 1988; Structure of an extracellular cross-reactive polysaccharide from Pseudomonas aeruginosa immunotype 4. J Biol Chem 263:11291–11295
     [Google Scholar]
  15. Lam M. Y., McGroarty E. J., Kropinski A. M., MacDonald L. A., Pedersen S. S., Høiby N., Lam J. S. 1989; Occurrence of a common lipopolysaccharide antigen in standard and clinical strains of Pseudomonas aeruginosa . J Clin Microbiol 27:962–967
     [Google Scholar]
  16. Likavcanova E., Lagace J. 1992; Quantitative analysis of immunoglobulin G subclass responses to Pseudomonas aeruginosa antigens in cystic fibrosis. J Med Microbiol 36:437–444 [CrossRef]
     [Google Scholar]
  17. McKie A., Vyse A., Maple C. 2002; Novel methods for the detection of microbial antibodies in oral fluid. Lancet Infect Dis 2:18–24 [CrossRef]
     [Google Scholar]
  18. Rehm B. H., Grabert E., Hein J., Winkler U. K. 1994; Antibody response of rabbits and cystic fibrosis patients to an alginate-specific outer membrane protein of a mucoid strain of Pseudomonas aeruginosa . Microb Pathog 16:43–51 [CrossRef]
     [Google Scholar]
  19. Tsai C. M., Frasch C. E. 1982; A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem 119:115–119 [CrossRef]
     [Google Scholar]
  20. West S. E., Zeng L., Lee B. L., Kosorok M. R., Laxova A., Rock M. J., Splaingard M. J., Farrell P. M. 2002; Respiratory infections with Pseudomonas aeruginosa in children with cystic fibrosis: early detection by serology and assessment of risk factors. JAMA 287:2958–2967 [CrossRef]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.46833-0
Loading
Detection of antibodies to Pseudomonas aeruginosa in serum and oral fluid from patients with cystic fibrosis
J. Med. Microbiol. 56, 670 (2007); https://doi.org/10.1099/jmm.0.46833-0
/content/journal/jmm/10.1099/jmm.0.46833-0
/content/journal/jmm/10.1099/jmm.0.46833-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error