1887

Abstract

Infections by species are an important cause of diarrhoeal disease worldwide. Of 4198 isolates received by the French National Reference Centre for and , 180 from patients with diarrhoea and dysentery in 2000–2004 did not react with any available polyclonal rabbit antisera used to identify the established serogroups. This study describes the molecular and phenotypic characteristics of these isolates in seroagglutination tests, molecular serotyping (-RFLP and -RFLP), ribotyping, detection of invasivity and enterotoxins genes, and antibiotic sensitivity. All isolates gave biochemical reactions typical of , were mannitol-positive and indole-negative. They all carried invasion-associated genes, enterotoxin 2 [ShET-2] and an IS sequence. They had a unique ribotype that was distinct from all other and patterns. Further characterization by -RFLP clearly distinguished this serogroup from all other or O-groups. The -RFLP pattern corresponded to P4, an F-pattern which is associated with 10 different serogroups of . A new antiserum prepared against strain 00-977 agglutinated all 180 isolates and cross-agglutination and absorption studies with anti-00-977 serum and anti-CDC 99-4528 (reference for the newly described serogroup 20) serum showed identical antigenic structure. Furthermore, strains 00-977 and CDC 99-4528 had the same molecular serotype, ribotype and virulence genes.

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2007-06-01
2019-11-22
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