1887

Abstract

is a dimorphic fungus endemic in southeast Asia. The incidence of infection has increased greatly in this region with the spread of human immunodeficiency virus, but the infection routes and pathogenic mechanisms of remain poorly understood. is an opportunistic human pathogen exhibiting a temperature-dependent dimorphic switch. At 25 °C it grows as filamentous hyphae, whilst at 37 °C it forms uninucleate yeast cells and divides by fission. Dimorphic fungal pathogenicity is frequently associated with the dimorphic switch, but the mechanism that regulates the switch has remained obscure. In this report, two-dimensional difference gel electrophoresis was used to investigate the proteins expressed differentially in the yeast and mycelial phases of a wild-type isolate of . Among thousands of protein molecules displayed, more than 500 showed differential expression between the two phases. In particular, 26 proteins were identified using matrix-assisted laser desorption/ionization time-of-flight MS. Expression of catalase-peroxidase, isocitrate lyase, Hsp90, binding protein and cytochrome P-450 increased significantly in the yeast phase, whereas levels of poly(A) polymerase and SNF22 were reduced.

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2007-03-01
2019-11-17
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References

  1. Borneman, A. R., Hynes, M. J. & Andrianopoulos, A. ( 2000; ). The abaA homologue of Penicillium marneffei participates in two developmental programmes: conidiation and dimorphic growth. Mol Microbiol 38, 1034–1047.
    [Google Scholar]
  2. Borneman, A. R., Hynes, M. J. & Andrianopoulos, A. ( 2001; ). An STE12 homolog from the asexual, dimorphic fungus Penicillium marneffei complements the defect in sexual development of an Aspergillus nidulans steA mutant. Genetics 157, 1003–1014.
    [Google Scholar]
  3. Choi, W., Yoo, Y. J., Kim, M., Shin, D., Jeon, H. B. & Choi, W. ( 2003; ). Identification of proteins highly expressed in the hyphae of Candida albicans by two-dimensional electrophoresis. Yeast 20, 1053–1060.[CrossRef]
    [Google Scholar]
  4. Cooper, C. R., Jr & Haycocks, N. G. ( 2000; ). Penicillium marneffei: an insurgent species among the penicillia. J Eukaryot Microbiol 47, 24–28.[CrossRef]
    [Google Scholar]
  5. de Voss, J. J., Rutter, K., Schroeder, B. G., Su, H., Zhu, Y. & Barry, C. E., III ( 2000; ). The salicylate-derived mycobactin siderophores of Mycobacterium tuberculosis are essential for growth in macrophages. Proc Natl Acad Sci U S A 97, 1252–1257.[CrossRef]
    [Google Scholar]
  6. Friedman, D. B., Hill, S., Keller, J. W., Merchant, N. B., Levy, S. E., Coffey, R. J. & Caprioli, R. M. ( 2004; ). Proteome analysis of human colon cancer by two-dimensional difference gel electrophoresis and mass spectrometry. Proteomics 4, 793–811.[CrossRef]
    [Google Scholar]
  7. Goldman, G. H., dos Reis Marques, E., Duarte Ribeiro, D. C., de Souza Bernardes, L. A., Quiapin, A. C., Vitorelli, P. M., Savoldi, M., Semighini, C. P., de Oliveira, R. C. & other authors ( 2003; ). Expressed sequence tag analysis of the human pathogen Paracoccidioides brasiliensis yeast phase: identification of putative homologues of Candida albicans virulence and pathogenicity genes. Eukaryot Cell 2, 34–48.[CrossRef]
    [Google Scholar]
  8. Grinyer, J., McKay, M., Nevalainen, H. & Herbert, B. R. ( 2004; ). Fungal proteomics: initial mapping of biological control strain Trichoderma harzianum. Curr Genet 45, 163–169.[CrossRef]
    [Google Scholar]
  9. Hamilton, A. J., Jeavons, L., Youngchim, S. & Vanittanakom, N. ( 1999; ). Recognition of fibronectin by Penicillium marneffei conidia via a sialic acid-dependent process and its relationship to the interaction between conidia and laminin. Infect Immun 67, 5200–5205.
    [Google Scholar]
  10. Hu, Y., Wang, G., Chen, G. Y. J., Fu, X. & Yao, S. Q. ( 2003; ). Proteome analysis of Saccharomyces cerevisiae under metal stress by two-dimensional differential gel electrophoresis. Electrophoresis 24, 1458–1470.[CrossRef]
    [Google Scholar]
  11. Kawasaki, L. & Aguirre, J. ( 2001; ). Multiple catalase genes are differentially regulated in Aspergillus nidulans. J Bacteriol 183, 1434–1440.[CrossRef]
    [Google Scholar]
  12. Leberer, E., Ziegelbauer, K., Schmidt, A., Harcus, D., Dignard, D., Ash, J., Johnson, L. & Thomas, D. Y. ( 1997; ). Virulence and hyphal formation of Candida albicans require the Ste20p-like protein kinase CaCla4p. Curr Biol 7, 539–546.[CrossRef]
    [Google Scholar]
  13. Li, P. C. & Yeoh, E. K. ( 1992; ). Current epidemiological trends of HIV infection in Asia. AIDS Clin Rev 1–23.
    [Google Scholar]
  14. Lorenz, M. C. & Fink, G. R. ( 2001; ). The glyoxylate cycle is required for fungal virulence. Nature 412, 83–86.[CrossRef]
    [Google Scholar]
  15. Lorenz, M. C. & Fink, G. R. ( 2002; ). Life and death in a macrophage: role of the glyoxylate cycle in virulence. Eukaryot Cell 1, 657–662.[CrossRef]
    [Google Scholar]
  16. McKinney, J. D., Höner zu Bentrup, K., Muñoz-Elias, E. J., Miczak, A., Chen, B., Chan, W.-T., Swenson, D., Sacchettini, J. C., Jacobs, W. R., Jr & Russell, D. G. ( 2000; ). Persistence of Mycobacterium tuberculosis in macrophages and mice requires the glyoxylate shunt enzyme isocitrate lyase. Nature 406, 735–738.[CrossRef]
    [Google Scholar]
  17. Paris, S., Wysong, D., Debeaupuis, J. P., Shibuya, K., Philippe, B., Diamond, R. D. & Latge, J. P. ( 2003; ). Catalases of Aspergillus fumigatus. Infect Immun 71, 3551–3562.[CrossRef]
    [Google Scholar]
  18. Pongpom, P., Cooper, C. R., Jr & Vanittanakom, N. ( 2005; ). Isolation and characterization of a catalase-peroxidase gene from the pathogenic fungus, Penicillium marneffei. Med Mycol 43, 403–411.[CrossRef]
    [Google Scholar]
  19. Rooney, P. J., Sullivan, T. D. & Klein, B. S. ( 2001; ). Selective expression of the virulence factor BAD1 upon morphogenesis to the pathogenic yeast form of Blastomyces dermatitidis: evidence for transcriptional regulation by a conserved mechanism. Mol Microbiol 39, 875–889.[CrossRef]
    [Google Scholar]
  20. Sebghati, T. S., Engle, J. T. & Goldman, W. E. ( 2000; ). Intracellular parasitism by Histoplasma capsulatum: fungal virulence and calcium dependence. Science 290, 1368–1372.[CrossRef]
    [Google Scholar]
  21. Supparatpinyo, K., Khamwan, C., Baosoung, V., Nelson, K. E. & Sirisanthana, T. ( 1994; ). Disseminated Penicillium marneffei infection in southeast Asia. Lancet 344, 110–113.[CrossRef]
    [Google Scholar]
  22. Todd, R. B., Greenhalgh, J. R., Hynes, M. J. & Andrianopoulos, A. ( 2003; ). TupA, the Penicillium marneffei Tup1p homologue, represses both yeast and spore development. Mol Microbiol 48, 85–94.[CrossRef]
    [Google Scholar]
  23. Trewatcharegon, S., Chaiyaroj, S. C., Chongtrakool, P. & Sirisinha, S. ( 2000; ). Production and characterization of monoclonal antibodies reactive with the mycelial and yeast phases of Penicillium marneffei. Med Mycol 38, 91–96.[CrossRef]
    [Google Scholar]
  24. Xi, L., Lu, C., Zhou, X., Wang, L. & Xie, S. ( 2004; ). Fifteen cases of penicilliosis in Guangdong, China. Mycopathologia 158, 151–155.[CrossRef]
    [Google Scholar]
  25. Zamocky, M. ( 2004; ). Phylogenetic relationships in class I of the superfamily of bacterial, fungal, and plant peroxidases. Eur J Biochem 271, 3297–3309.[CrossRef]
    [Google Scholar]
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