RT Journal Article SR Electronic(1) A1 Qin, Zhiqiang A1 Yang, Xiaomei A1 Yang, Lei A1 Jiang, Juan A1 Ou, Yuanzhu A1 Molin, Soeren A1 Qu, DiYR 2007 T1 Formation and properties of in vitro biofilms of ica-negative Staphylococcus epidermidis clinical isolates JF Journal of Medical Microbiology, VO 56 IS 1 SP 83 OP 93 DO https://doi.org/10.1099/jmm.0.46799-0 PB Microbiology Society, SN 1473-5644, AB Coagulase-negative Staphylococcus epidermidis has become the leading cause of foreign-body infections due to its biofilm formation on all kinds of medical-device surfaces. The biofilm development of S. epidermidis includes two steps: the initial attachment phase and the accumulative phase. In the accumulative phase, the polysaccharide intercellular adhesin (PIA), encoded by the icaADBC locus, is the major component mediating intercellular adhesion. However, recent studies have revealed the emergence of biofilm-positive/ica-negative staphylococcal clinical isolates. In this report, two ica-negative S. epidermidis clinical strains, SE1 and SE4, exhibited their heterogeneity in biofilm architecture under static and flow conditions, compared with the biofilm-positive/ica-positive RP62A strain. Strains with this type of absence of PIA from biofilms also displayed intermediate resistance to vancomycin. More importantly, the cells of both SE1 and SE4 strains were more tolerant than those of RP62A to exposure to lysostaphin and vancomycin. Based on the results, it is suggested that the biofilm-positive/ica-negative strain represents a newly emergent subpopulation of S. epidermidis clinical strains, arising from selection by antibiotics in the nosocomial milieu, which displays a survival advantage in its host environment. Recent epidemiological data support this suggestion, by showing a tendency towards an increasing proportion of this subpopulation in staphylococci-associated infections., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.46799-0