1887

Abstract

subspecies serovar Paratyphi B [O1,4,(5),12 : Hb : 1,2] can cause either an enteric fever (paratyphoid fever) or self-limiting gastroenteritis in humans. The -tartrate non-fermenting variant subsp. serovar Paratyphi B dT (. Paratyphi B) is the causative agent of paratyphoid fever, and the -tartrate fermenting variant subsp. serovar Paratyphi B dT (. Paratyphi B dT; formerly called Java) causes gastroenteritis. . Java is currently recognized as an emerging problem worldwide. Twelve dT . Java isolates were collected in Indonesia between 2000 and 2002. One-third of them contained genomic island 1 (SGI1), which gives the multidrug-resistant phenotype to the bacteria. In this study, a PCR-based method to detect a single nucleotide difference responsible for the inability to ferment -tartrate, reported elsewhere, was validated. The -tartrate fermenting phenotype of . Java was converted to the non-fermenting phenotype by the disruption of the ORF STM 3356, and the -tartrate non-fermenting phenotype of the ORF STM 3356-disrupted strain and the dT reference strain was changed to the dT phenotype by complementing ORF STM 3356 . The results show that the dT phenotype requires a functional product encoded by STM 3356, and support the use of the PCR-based discrimination method for . Paratyphi B and . Java as the standard differentiation method.

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2006-12-01
2020-10-28
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