1887

Abstract

Frequent outbreaks of fungaemia in paediatric patients have warranted the development of a rapid identification system for this organism. This study describes a specific PCR-based method targeting the rRNA gene intergenic spacer region 1 (IGS1) for rapid identification of isolates and characterization at the strain level. These methods of species identification and strain typing were used on 106 isolates of (77 from a previously described outbreak and 29 isolated post-outbreak from the same hospital). Using conventional morphological and biochemical methods, 11 strains isolated during the outbreak were misidentified as . analysis of sequences of internal transcribed spacer (ITS) regions of rRNA genes confirmed that they were (eight isolates) and (three isolates). Strain typing of isolates confirmed the previous finding of a point-source outbreak. The results suggest that IGS sequences and their polymorphisms could be exploited for similar typing methods in other organisms.

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2007-02-01
2019-10-17
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