1887

Abstract

An immunogenic 22 kilodalton exported subspecies (MAP) lipoprotein (P22) was previously identified, and found to belong to the LppX/LprAFG family of mycobacterial lipoproteins. N-terminal polyhistidine-tagged P22 was produced and purified from . Antibody recognition of P22, and interferon-gamma (IFN-) responses using blood from a sheep vaccinated with Neoparasec, confirmed its immunogenicity. To evaluate the immunogenicity of P22 , five sheep were immunized with a single dose containing 0.8 mg recombinant P22 protein in adjuvant. Blood was collected at 4, 13 and 29 weeks post-immunization (p.i.) and tested for anti-P22 antibodies and P22-specific IFN- production. P22-specific antibodies were detected by Western blot analysis in all five Neoparasec-immunized sheep at the three time points. Three out of five P22-immunized sheep produced P22-specific antibodies for up to 13 weeks p.i., and two gave a response at 29 weeks p.i. Recombinant P22 was able to stimulate significant IFN- production in blood of P22-immunized sheep at 13 and 29 weeks p.i. Recombinant P22 also elicited an IFN- response in blood of sheep immunized with Neoparasec.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.46785-0
2006-12-01
2019-11-14
Loading full text...

Full text loading...

/deliver/fulltext/jmm/55/12/1735.html?itemId=/content/journal/jmm/10.1099/jmm.0.46785-0&mimeType=html&fmt=ahah

References

  1. Begg, D. J. & Griffin, J. F. ( 2005; ). Vaccination of sheep against M. paratuberculosis: immune parameters and protective efficacy. Vaccine 73, 5603–5611.
    [Google Scholar]
  2. Bigi, F., Gioffré, A., Klepp, L. & 8 other authors ( 2004; ). The knockout of the lprG-Rv1410 operon produces strong attenuation of Mycobacterium tuberculosis. Microbes Infect 6, 182–187.[CrossRef]
    [Google Scholar]
  3. Chambers, M. A., Gavier-Widen, D. & Hewinson, R. G. ( 2004; ). Antibody bound to the surface antigen MPB83 of Mycobacterium bovis enhances survival against high dose and low dose challenge. FEMS Immunol Med Microbiol 41, 93–100.[CrossRef]
    [Google Scholar]
  4. Collett, M. W. & West, D. M. ( 2001; ). A comparison of the injection site and draining lymph node pathology induced by an attenuated live and killed Johne's disease vaccine in sheep. In Proceedings of the 5th International Sheep Veterinary Congress, pp. 28–29. Stellenbosch, South Africa.
  5. Collins, M. T. ( 2003; ). Paratuberculosis: review of present knowledge. Acta Vet Scand 44, 217–221.
    [Google Scholar]
  6. Dupont, C., Thompson, K., Heuer, C., Gicquel, B. & Murray, A. ( 2005; ). Identification and characterization of an immunogenic 22 kDa exported protein of Mycobacterium avium subspecies paratuberculosis. J Med Microbiol 54, 1083–1092.[CrossRef]
    [Google Scholar]
  7. Emery, D. L. & Whittington, R. J. ( 2004; ). An evaluation of mycophage therapy, chemotherapy and vaccination for control of Mycobacterium avium subsp. paratuberculosis infection. Vet Microbiol 104, 143–155.[CrossRef]
    [Google Scholar]
  8. Gehring, A. J., Dobos, K. M., Belisle, J. T., Harding, C. V. & Boom, W. H. ( 2004; ). Mycobacterium tuberculosis LprG (Rv1411c): a novel TLR-2 ligand that inhibits human macrophage class II MHC antigen processing. J Immunol 173, 2660–2668.[CrossRef]
    [Google Scholar]
  9. Harris, N. B. & Barletta, R. G. ( 2001; ). Mycobacterium avium subsp. paratuberculosis in veterinary medicine. Clin Microbiol Rev 14, 489–512.[CrossRef]
    [Google Scholar]
  10. Hovav, A. H., Mullerad, J., Davidovitch, L., Fishman, Y., Bigi, F., Cataldi, A. & Bercovier, H. ( 2003; ). The Mycobacterium tuberculosis recombinant 27-kilodalton lipoprotein induces a strong Th1-type immune response deleterious to protection. Infect Immun 71, 3146–3154.[CrossRef]
    [Google Scholar]
  11. Huntley, J. F., Stabel, J. R. & Bannantine, J. P. ( 2005; ). Immunoreactivity of the Mycobacterium avium subsp. paratuberculosis 19-kDa lipoprotein. BMC Microbiol 5, 3.[CrossRef]
    [Google Scholar]
  12. Kalis, C. H., Hesselink, J. W., Barkema, H. W. & Collins, M. T. ( 2001; ). Use of long-term vaccination with a killed vaccine to prevent fecal shedding of Mycobacterium avium subsp. paratuberculosis in dairy herds. Am J Vet Res 62, 270–274.[CrossRef]
    [Google Scholar]
  13. Klawonn, W., Cussler, K., Drager, K. G., Gyra, H., Kohler, H., Zimmer, K. & Hess, R. G. ( 2002; ). The importance of allergic skin test with Johnin, antibody ELISA, cultural fecal test as well as vaccination for the sanitation of three chronically paratuberculosis-infected dairy herds in Rhineland-Palatinate. Dtsch Tieraerztl Wochenschr 109, 510–516 (in German).
    [Google Scholar]
  14. Kohler, H., Gyra, H., Zimmer, K. & 7 other authors ( 2001; ). Immune reactions in cattle after immunization with a Mycobacterium paratuberculosis vaccine and implications for the diagnosis of M. paratuberculosis and M. bovis infections. J Vet Med B Infect Dis Vet Public Health 48, 185–195.[CrossRef]
    [Google Scholar]
  15. Kurade, N. P., Tripathi, B. N., Rajukumar, K. & Parihar, N. S. ( 2004; ). Sequential development of histologic lesions and their relationship with bacterial isolation, fecal shedding, and immune responses during progressive stages of experimental infection of lambs with Mycobacterium avium subsp. paratuberculosis. Vet Pathol 41, 378–387.[CrossRef]
    [Google Scholar]
  16. Littell, R. C., Henry, P. R. & Ammerman, C. B. ( 1998; ). Statistical analysis of repeated measures data using SAS procedures. J Anim Sci 76, 1216–1231.
    [Google Scholar]
  17. Mullerad, J., Hovav, A. H., Fishman, Y., Barletta, R. G. & Bercovier, H. ( 2002a; ). Antigenicity of Mycobacterium paratuberculosis superoxide dismutase in mice. FEMS Immunol Med Microbiol 34, 81–88.[CrossRef]
    [Google Scholar]
  18. Mullerad, J., Michal, I., Fishman, Y., Hovav, A. H., Barletta, R. G. & Bercovier, H. ( 2002b; ). The immunogenicity of Mycobacterium paratuberculosis 85B antigen. Med Microbiol Immunol 190, 179–187.[CrossRef]
    [Google Scholar]
  19. Mullerad, J., Hovav, A. H., Nahary, R., Fishman, Y. & Bercovier, H. ( 2003; ). Immunogenicity of a 16.7 kDa Mycobacterium paratuberculosis antigen. Microb Pathog 34, 81–90.[CrossRef]
    [Google Scholar]
  20. Muskens, J., van Zijderveld, F., Eger, A. & Bakker, D. ( 2002; ). Evaluation of the long-term immune response in cattle after vaccination against paratuberculosis in two Dutch dairy herds. Vet Microbiol 86, 269–278.[CrossRef]
    [Google Scholar]
  21. Olsen, I. & Storset, A. K. ( 2001; ). Innate IFN-gamma production in cattle in response to MPP14, a secreted protein from Mycobacterium avium subsp. paratuberculosis. Scand J Immunol 54, 306–313.[CrossRef]
    [Google Scholar]
  22. Pethe, K., Alonso, S., Biet, F., Delogu, G., Brennan, M. J., Locht, C. & Menozzi, F. D. ( 2001; ). The heparin-binding haemagglutinin of M. tuberculosis is required for extrapulmonary dissemination. Nature 412, 190–194.[CrossRef]
    [Google Scholar]
  23. Sigurethardottir, O. G., Valheim, M. & Press, C. M. ( 2004; ). Establishment of Mycobacterium avium subsp. paratuberculosis infection in the intestine of ruminants. Adv Drug Deliv Rev 56, 819–834.[CrossRef]
    [Google Scholar]
  24. Teitelbaum, R., Glatman-Freedman, A., Chen, B., Robbins, J. B., Unanue, E., Casadevall, A. & Bloom, B. R. ( 1998; ). A mAb recognizing a surface antigen of Mycobacterium tuberculosis enhances host survival. Proc Natl Acad Sci U S A 95, 15688–15693.[CrossRef]
    [Google Scholar]
  25. Temmerman, S., Pethe, K., Parra, M. & 11 other authors ( 2004; ). Methylation-dependent T cell immunity to Mycobacterium tuberculosis heparin-binding hemagglutinin. Nat Med 10, 935–941.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.46785-0
Loading
/content/journal/jmm/10.1099/jmm.0.46785-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error