Relationship between O-antigen subtypes, bacterial surface structures and O-antigen gene clusters in O123 strains carrying genes for Shiga toxins and intimin Free

Abstract

O123 strains express a broad spectrum of phenotypes, H serotypes and virulence markers and are able to colonize and to cause disease in different hosts including humans. In this study, two subtypes of O123 antigen (group I and group II) have been identified based on their cross-reactions with other O antigens. Investigation of the relationship between O123 group I and group II strains by O serotyping and Fourier transform infrared spectroscopy of whole bacteria revealed surface structural differences between these two groups of O123 strains. Nucleotide sequence analysis of the O-antigen gene clusters of two O123 strains representing O123 group I and group II revealed no change at the amino acid level. These findings indicate that the differences in the surface structures of group I and group II strains are not related to genetic heterogeneity in their O-antigen gene clusters. A PCR assay based on O123 antigen-specific and genes was developed and found to be suitable for reliable detection of all subtypes of O123 strains, which bears an advantage over traditional serological detection.

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2007-02-01
2024-03-28
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References

  1. Bastin D. A., Reeves P. R. 1995; Sequence and analysis of the O antigen gene ( rfb ) cluster of Escherichia coli O111. Gene 164:17–23 [CrossRef]
    [Google Scholar]
  2. Beutin L., Geier D., Steinruck H., Zimmermann S., Scheutz F. 1993; Prevalence and some properties of verotoxin (Shiga-like toxin)-producing Escherichia coli in seven different species of healthy domestic animals. J Clin Microbiol 31:2483–2488
    [Google Scholar]
  3. Beutin L., Krause G., Zimmermann S., Kaulfuss S., Gleier K. 2004; Characterization of Shiga toxin-producing Escherichia coli strains isolated from human patients in Germany over a 3-year period. J Clin Microbiol 42:1099–1108 [CrossRef]
    [Google Scholar]
  4. Beutin L., Tao J., Feng L., Krause G., Zimmermann S., Gleier K., Xia Q., Wang L. 2005; Sequence analysis of the Escherichia coli O15 antigen gene cluster and development of a PCR assay for rapid detection of intestinal and extraintestinal pathogenic E. coli O15 strains. J Clin Microbiol 43:703–710 [CrossRef]
    [Google Scholar]
  5. Brett K. N., Ramachandran V., Hornitzky M. A., Bettelheim K. A., Walker M. J., Djordjevic S. P. 2003; stx 1c is the most common Shiga toxin 1 subtype among Shiga toxin-producing Escherichia coli isolates from sheep but not among isolates from cattle. J Clin Microbiol 41:926–936 [CrossRef]
    [Google Scholar]
  6. Daniels C., Vindurampulle C., Morona R. 1998; Overexpression and topology of the Shigella flexneri O-antigen polymerase (Rfc/Wzy). Mol Microbiol 28:1211–1222 [CrossRef]
    [Google Scholar]
  7. Djordjevic S. P., Ramachandran V., Bettelheim K. A., Vanselow B. A., Holst P., Bailey G., Hornitzky M. A. 2004; Serotypes and virulence gene profiles of shiga toxin-producing Escherichia coli strains isolated from feces of pasture-fed and lot-fed sheep. Appl Environ Microbiol 70:3910–3917 [CrossRef]
    [Google Scholar]
  8. D'Souza J. M., Wang L., Reeves P. R. 2002; Sequence of the Escherichia coli O26 antigen gene cluster and identification of O26 specific genes. Gene 297:123–127 [CrossRef]
    [Google Scholar]
  9. D'Souza J. M., Samuel G. N., Reeves P. R. 2005; Evolutionary origins and sequence of the Escherichia coli O4 O-antigen gene cluster. FEMS Microbiol Lett 244:27–32 [CrossRef]
    [Google Scholar]
  10. Ewing W. H. 1986 Edwards and Ewing's Identification of the Enterobacteriaceae , 4th edn. Amsterdam: Elsevier;
    [Google Scholar]
  11. Feng L., Senchenkova S. N., Yang J., Shashkov A. S., Tao J., Guo H., Zhao G., Knirel Y. A., Reeves P., Wang L. 2004a; Structural and genetic characterization of the Shigella boydii type 13 O antigen. J Bacteriol 186:383–392 [CrossRef]
    [Google Scholar]
  12. Feng L., Tao J., Guo H., Xu J., Li Y., Rezwan F., Reeves P. R., Wang L. 2004b; Structure of the Shigella dysenteriae 7 O antigen gene cluster and identification of its antigen specific genes. Microb Pathog 36:109–115 [CrossRef]
    [Google Scholar]
  13. Feng L., Wang W., Tao J., Guo H., Krause G., Beutin L., Wang L. 2004c; Identification of Escherichia coli O114 O-antigen gene cluster and development of an O114 serogroup-specific PCR assay. J Clin Microbiol 42:3799–3804 [CrossRef]
    [Google Scholar]
  14. Guo H., Feng L., Tao J., Zhang C., Wang L. 2004; Identification of Escherichia coli O172 O-antigen gene cluster and development of a serogroup-specific PCR assay. J Appl Microbiol 97:181–190 [CrossRef]
    [Google Scholar]
  15. Helm D., Labischinski H., Naumann D. 1991a; Elaboration of a procedure for identification of bacteria using Fourier-transform infrared spectral libraries: a stepwise correlation approach. J Microbiol Methods 14:127–142 [CrossRef]
    [Google Scholar]
  16. Helm D., Labischinski H., Schallehn G., Naumann D. 1991b; Classification and identification of bacteria by Fourier-transform infrared spectroscopy. J Gen Microbiol 137:69–79 [CrossRef]
    [Google Scholar]
  17. Horbach I., Naumann D., Fehrenbach F. J. 1988; Simultaneous infections with different serogroups of Legionella pneumophila investigated by routine methods and Fourier transform infrared spectroscopy. J Clin Microbiol 26:1106–1110
    [Google Scholar]
  18. Kirschner C., Maquelin K., Pina P., Ngo Thi N. A., Choo-Smith L. P., Sockalingum G. D., Sandt C., Ami D., Orsini F. other authors 2001; Classification and identification of enterococci: a comparative phenotypic, genotypic, and vibrational spectroscopic study. J Clin Microbiol 39:1763–1770 [CrossRef]
    [Google Scholar]
  19. Kneidinger B., Larocque S., Brisson J., Cadotte N., Lam J. S. 2003; Biosynthesis of 2-acetamido-2,6-dideoxy-l-hexoses in bacteria follows a pattern distinct from those of the pathways of 6-deoxy-l-hexoses. Biochem J 371:989–995 [CrossRef]
    [Google Scholar]
  20. Krause G., Zimmermann S., Beutin L. 2005; Investigation of domestic animals and pets as a reservoir for intimin- ( eae ) gene positive Escherichia coli types. Vet Microbiol 106:87–95 [CrossRef]
    [Google Scholar]
  21. Leomil L., Aidar-Ugrinovich L., Guth B. E., Irino K., Vettorato M. P., Onuma D. L., de Castro A. F. 2003; Frequency of Shiga toxin-producing Escherichia coli (STEC) isolates among diarrheic and non-diarrheic calves in Brazil. Vet Microbiol 97:103–109 [CrossRef]
    [Google Scholar]
  22. Liu D., Cole R., Reeves P. R. 1996; An O-antigen processing function for Wzx(RfbX): a promising candidate for O-unit flippase. J Bacteriol 178:2102–2107
    [Google Scholar]
  23. Malik A., Toth I., Beutin L., Schmidt H., Taminiau B., Dow M. A., Morabito S., Oswald E., Mainil J., Nagy B. 2006; Serotypes and intimin types of intestinal and faecal strains of eae + Escherichia coli from weaned pigs. Vet Microbiol 114:82–93 [CrossRef]
    [Google Scholar]
  24. Maquelin K., Kirschner C., Choo-Smith L. P., Ngo-Thi N. A., van Vreeswijk T., Stammler M., Endtz H. P., Bruining H. A., Naumann D., Puppels G. J. 2003; Prospective study of the performance of vibrational spectroscopies for rapid identification of bacterial and fungal pathogens recovered from blood cultures. J Clin Microbiol 41:324–329 [CrossRef]
    [Google Scholar]
  25. Marolda C. L., Feldman M. F., Valvano M. A. 1999; Genetic organization of the O7-specific lipopolysaccharide biosynthesis cluster of Escherichia coli VW187 (O7 : K1). Microbiology 145:2485–2495
    [Google Scholar]
  26. Mercado E. C., Gioffre A., Rodriguez S. M., Cataldi A., Irino K., Elizondo A. M., Cipolla A. L., Romano M. I., Malena R., Mendez M. A. 2004; Non-O157 Shiga toxin-producing Escherichia coli isolated from diarrhoeic calves in Argentina. J Vet Med B Infect Dis Vet Public Health 51:82–88 [CrossRef]
    [Google Scholar]
  27. Naumann D. 2000; Infrared spectroscopy in microbiology. In Encyclopedia of Analytical Chemistry pp  102–131 Edited by Meyers R. A. Chichester: Wiley;
    [Google Scholar]
  28. Naumann D., Helm D., Labischinski H. 1991; Microbiological characterizations by FT-IR spectroscopy. Nature 351:81–82 [CrossRef]
    [Google Scholar]
  29. Orden J. A., Ruiz-Santa-Quiteria J. A., Cid D., Garcia S., Sanz R., de la Fuente R. 1998; Verotoxin-producing Escherichia coli (VTEC) and eae -positive non-VTEC in 1-30-days-old diarrhoeic dairy calves. Vet Microbiol 63:239–248 [CrossRef]
    [Google Scholar]
  30. Orskov F. 1952; Antigenic relationships between o groups 1-112 of E. coli and Wramby's o groups 26w-43w. 10 new o groups: 114-123. Acta Pathol Microbiol Scand 31:51–56
    [Google Scholar]
  31. Orskov F., Orskov I. 1984 Serotyping of Escherichia coli London: Academic Press;
    [Google Scholar]
  32. Orskov I. F., Orskov F. B., Jann B., Jann K. 1977; Serology, chemistry, and genetics of O and K antigens of Escherichia coli . Bacteriol Rev 41:667–710
    [Google Scholar]
  33. Ramachandran V., Hornitzky M. A., Bettelheim K. A., Walker M. J., Djordjevic S. P. 2001; The common ovine Shiga toxin 2-containing Escherichia coli serotypes and human isolates of the same serotypes possess a Stx2d toxin type. J Clin Microbiol 39:1932–1937 [CrossRef]
    [Google Scholar]
  34. Reeves P. R. 1995; Role of O-antigen variation in the immune response. Trends Microbiol 3:381–386 [CrossRef]
    [Google Scholar]
  35. Reeves P. R., Wang L. 2002; Genomic organization of LPS-specific loci. Curr Top Microbiol Immunol 264:109–135
    [Google Scholar]
  36. Tintelnot K., Haase G., Seibold M., Bergmann F., Staemmler M., Franz T., Naumann D. 2000; Evaluation of phenotypic markers for selection and identification of Candida dubliniensis . J Clin Microbiol 38:1599–1608
    [Google Scholar]
  37. Wang L., Reeves P. R. 1998; Organization of Escherichia coli O157 O antigen gene cluster and identification of its specific genes. Infect Immun 66:3545–3551
    [Google Scholar]
  38. Wang L., Reeves P. R. 2000; The Escherichia coli O111 and Salmonella enterica O35 gene clusters: gene clusters encoding the same colitose-containing O antigen are highly conserved. J Bacteriol 182:5256–5261 [CrossRef]
    [Google Scholar]
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