1887

Abstract

O123 strains express a broad spectrum of phenotypes, H serotypes and virulence markers and are able to colonize and to cause disease in different hosts including humans. In this study, two subtypes of O123 antigen (group I and group II) have been identified based on their cross-reactions with other O antigens. Investigation of the relationship between O123 group I and group II strains by O serotyping and Fourier transform infrared spectroscopy of whole bacteria revealed surface structural differences between these two groups of O123 strains. Nucleotide sequence analysis of the O-antigen gene clusters of two O123 strains representing O123 group I and group II revealed no change at the amino acid level. These findings indicate that the differences in the surface structures of group I and group II strains are not related to genetic heterogeneity in their O-antigen gene clusters. A PCR assay based on O123 antigen-specific and genes was developed and found to be suitable for reliable detection of all subtypes of O123 strains, which bears an advantage over traditional serological detection.

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2007-02-01
2019-11-22
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