1887

Abstract

Previously, it has been demonstrated that the invasion of Caco-2 cells by induces apoptotic cell death. Therefore, the attachment to and colonization of eukaryotic intestinal host cells by are important steps in causing pathogenicity. In this study, the participation of GroEL in the attachment of was examined. The operon of was isolated by PCR. The nucleotide sequence of the operon of revealed two ORFs of 294 nucleotides for and 1647 nucleotides for . Cell fractionation and immunostaining experiments suggested that the GroEL of was associated with the bacterial cell surface. The expression of the gene was upregulated during the attachment and apoptosis-induction stages, and the expression of the protein was also induced during the attachment stage. Furthermore, GroEL efficiently promoted the attachment of to Caco-2 cells, as measured by a FACSCalibur flow cytometer. These results demonstrated that GroEL has a positive influence on the attachment of to Caco-2 cells.

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2007-01-01
2019-11-12
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References

  1. Avadhanula, V., Rodriguez, C. A., Ulett, G. C., Bakaletz, L. O. & Adderson, E. E. ( 2006; ). Nontypeable Haemophilus influenzae adheres to intercellular adhesion molecule 1 (ICAM-1) on respiratory epithelial cells and upregulates ICAM-1 expression. Infect Immun 74, 830–838.[CrossRef]
    [Google Scholar]
  2. Brenden, R. A., Miller, M. A. & Janda, J. M. ( 1998; ). Clinical disease spectrum and pathogenic factors associated with Plesiomonas shigelloides infections in humans. Rev Infect Dis 10, 303–316.
    [Google Scholar]
  3. Ensgraber, M. & Loos, M. ( 1992; ). A 66-kilodalton heat shock protein of Salmonella typhimurium is responsible for binding of the bacterium to intestinal mucus. Infect Immun 60, 3072–3078.
    [Google Scholar]
  4. Falcon, R., Carbonell, G. V., Figueredo, P. M. S., Butiao, F., Saridakis, H. O., Pelayo, J. S. & Yano, T. ( 2003; ). Intracellular vacuolation induced by culture filtrates of Plesiomonas shigelloides isolated from environmental sources. J Appl Microbiol 95, 273–278.[CrossRef]
    [Google Scholar]
  5. Frisk, A., Ison, C. A. & Lagergard, T. ( 1998; ). GroEL heat shock protein of Haemophilus ducreyi: association with cell surface and capacity to bind to eukaryotic cells. Infect Immun 66, 1252–1257.
    [Google Scholar]
  6. Galdiero, M., Delero, G. C. & Marcatili, A. ( 1997; ). Cytokine and adhesion molecule expression in human monocytes and endothelial cells stimulated with bacterial heat shock proteins. Infect Immun 65, 699–707.
    [Google Scholar]
  7. Garduno, R. A., Faulkner, G., Trevors, M. A., Vats, N. & Hoffman, P. S. ( 1998a; ). Immunolocalization of Hsp60 in Legionella pneumophila. J Bacteriol 180, 505–513.
    [Google Scholar]
  8. Garduno, R. A., Garduno, E. & Hoffman, P. S. ( 1998b; ). Surface-associated Hsp60 chaperonin of Legionella pneumophila mediates invasion in a HeLa cell model. Infect Immun 66, 4602–4610.
    [Google Scholar]
  9. Goulhen, F., Hafezi, A., Uitto, V. J., Hinode, D., Nakamura, R., Grenier, D. & Mayrand, D. ( 1998; ). Subcellular localization and cytotoxic activity of the GroEL-like protein isolated from Actinobacillus actinomycetemcomitans. Infect Immun 66, 5307–5313.
    [Google Scholar]
  10. Hennequin, C., Collignon, A. & Karjalainen, T. ( 2001a; ). Analysis of expression of GroEL (Hsp60) of Clostridium difficile in response to stress. Microb Pathog 31, 255–260.[CrossRef]
    [Google Scholar]
  11. Hennequin, C., Porcheray, F., Waligora-Dupriet, A. J., Collignon, A., Barc, M. C., Bourlioux, P. & Karjalainen, T. ( 2001b; ). GroEL (Hsp60) of Clostridium difficile is involved in cell adherence. Microbiology 147, 87–96.
    [Google Scholar]
  12. Janda, J. M. & Abbott, S. L. ( 1993; ). Expression of hemolytic activity by Plesiomonas shigelloides. J Clin Microbiol 31, 1206–1208.
    [Google Scholar]
  13. Kaiserlian, D., Rigal, D., Abello, J. & Revillard, J. P. ( 1991; ). Expression, function and regulation of the intercellular adhesion molecule-1 (ICAM-1) on human intestinal epithelial cell lines. Eur J Immunol 21, 2415–2421.[CrossRef]
    [Google Scholar]
  14. Kirn, T. J., Jude, B. A. & Taylor, R. K. ( 2005; ). A colonization factor links Vibrio cholerae environmental survival and human infection. Nature 438, 863–866.[CrossRef]
    [Google Scholar]
  15. Marcatili, A., Cipollaro de l'Ero, G., Galdiero, M., Folgore, A. & Petrillo, G. ( 1997; ). TNF-α, IL-1α, IL-6 and ICAM-1 expression in human keratinocytes stimulated in vitro with Escherichia coli heat-shock proteins. Microbiology 143, 45–53.[CrossRef]
    [Google Scholar]
  16. Martinez-Murcia, A. J., Benlloch, S. & Collins, M. D. ( 1992; ). Phylogenetic interrelationships of members of the genera Aeromonas and Plesiomonas as determined by 16S ribosomal DNA sequencing: lack of congruence with results of DNA–DNA hybridizations. Int J Syst Bacteriol 42, 412–421.[CrossRef]
    [Google Scholar]
  17. Okawa, Y., Ohtomo, Y., Tsugawa, H., Matsuda, Y., Kobayashi, H. & Tsukamoto, T. ( 2004; ). Isolation and characterization of a cytotoxin produced by Plesiomonas shigelloides P-1 strain. FEMS Microbiol Lett 239, 125–130.[CrossRef]
    [Google Scholar]
  18. Paju, S., Goulhen, F., Asikainen, S., Grenier, D., Mayrand, D. & Uitto, V. J. ( 2000; ). Localization of heat shock proteins in clinical Actinobacillus actinomycetemcomitans strains and their effects on epithelial cell proliferation. FEMS Microbiol Lett 182, 231–235.[CrossRef]
    [Google Scholar]
  19. Phadnis, S. H., Parlow, M. H., Levy, M., Ilver, D., Caulkins, C. M., Connors, J. B. & Dunn, B. E. ( 1996; ). Surface localization of Helicobacter pylori urease and a heat shock protein homolog requires bacterial autolysis. Infect Immun 64, 905–912.
    [Google Scholar]
  20. Retzlaff, C., Yamamoto, Y., Hoffman, P. S., Friedman, H. & Klein, T. W. ( 1994; ). Bacterial heat shock proteins directly induced cytokine mRNA and interleukin-1 secretion in macrophage cultures. Infect Immun 62, 5689–5693.
    [Google Scholar]
  21. Saraswathi, B., Agarwal, R. K. & Sanyal, S. C. ( 1983; ). Further studies on enteropathogenicity of Plesiomonas shigelloides. Indian J Med Res 78, 12–18.
    [Google Scholar]
  22. Theodoropoulos, C., Wong, T. H., O'Brien, M. & Stenzel, D. ( 2001; ). Plesiomonas shigelloides enters polarized human intestinal Caco-2 cells in an in vitro model system. Infect Immun 69, 2260–2269.[CrossRef]
    [Google Scholar]
  23. Tsugawa, H., Ono, T., Murakami, H. & Okawa, Y. ( 2005; ). Invasive phenotype and apoptosis induction of Plesiomonas shigelloides P-1 strain to Caco-2 cells. J Appl Microbiol 99, 1435–1443.[CrossRef]
    [Google Scholar]
  24. Tsukamoto, T., Kinoshita, Y., Shimada, T. & Sakazaki, R. ( 1978; ). Two epidemics of diarrhoeal disease possibly caused by Plesiomonas shigelloides. J Hyg Camb 80, 275–280.[CrossRef]
    [Google Scholar]
  25. Wang, X. T., Nagaba, Y., Cross, H. S., Wrba, F., Zhang, L. & Guggino, S. E. ( 2000; ). The mRNA of L-type calcium channel elevated in colon cancer. Am J Pathol 157, 1549–1562.[CrossRef]
    [Google Scholar]
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