1887

Abstract

This study investigated the comparative performance of the Amplicor assay and an in-house semi-automated, multiplex real-time PCR for the diagnosis of genital chlamydial infection. Four different assays, the COBAS Amplicor CT test (Amplicor PCR), in-house real-time PCR (IHRT-PCR), in-house nested cryptic plasmid PCR and in-house nested major outer membrane protein PCR, were performed on genital swabs from 1000 consecutive patients attending a genitourinary medicine clinic. The samples were designated true positive if DNA was detected by at least two of the four above-mentioned assays while a sample was defined as true negative if DNA was detected in only one or none of the assays. By this criterion, there were 129 true positive and 871 true negative samples for DNA in this cohort. Amplicor PCR designated 144 samples positive: 128 (89 %) of 144 samples were true positive and 16 (11 %) were false positive. IHRT-PCR detected 126 of 129 true positive samples and did not generate any false positive results. The sensitivity of IHRT-PCR was comparable with, and specificity was higher than, Amplicor PCR for the diagnosis of genital chlamydial infection.

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2007-03-01
2019-11-22
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References

  1. Castriciano, S., Luinstra, K., Jang, D., Patel, J., Mahony, J., Kapala, J. & Chernesky, M. ( 2002; ). Accuracy of results obtained by performing a second ligase chain reaction assay and PCR analysis on urine samples with positive or near-cutoff results in the LCx test for Chlamydia trachomatis. J Clin Microbiol 40, 2632–2634.[CrossRef]
    [Google Scholar]
  2. Cates, W. & Wasserheit, J. N. ( 1991; ). Genital chlamydial infections: epidemiology and reproductive sequelae. Am J Obstet Gynecol 164, 1771–1781.[CrossRef]
    [Google Scholar]
  3. Centers for Disease Control and Prevention ( 2002; ). Screening tests to detect Chlamydia trachomatis and Neisseria gonorrhoeae infections – 2002. Morbid Mortal Wkly Rep 51 (No. RR-15), 1–39.
    [Google Scholar]
  4. Culler, E. E., Caliendo, A. M. & Nolte, F. S. ( 2003; ). Reproducibility of positive test results in BDProbeTec ET system for detection of Chlamydia trachomatis and Neisseria gonorrhoeae. J Clin Microbiol 41, 3911–3914.[CrossRef]
    [Google Scholar]
  5. Department of Health ( 2002; ). The National Strategy for Sexual Health and HIV: Implementation Action Plan. London: Department of Health.
  6. Health Protection Agency ( 2005a; ). Mapping the Issues. HIV and Other Sexually Transmitted Infections in the United Kingdom. The UK Collaborative Group for HIV and STI Surveillance. London: Health Protection Agency, Centre for Infections.
  7. Health Protection Agency ( 2005b; ). VSOP37i1. Chlamydia Infection Testing by Nucleic Acid Amplification Tests (NAATs). www.hpa-standardmethods.org.uk
  8. Jalal, H., Stephen, H., Al-Suwaine, A., Sonnex, C. & Carne, C. ( 2006a; ). The superiority of polymerase chain reaction over an amplified enzyme immunoassay for the detection of genital chlamydial infections. Sex Transm Infect 82, 37–40.[CrossRef]
    [Google Scholar]
  9. Jalal, H., Stephen, H., Curran, M. D., Burton, J., Bradley, M. & Carne, C. ( 2006b; ). Development and validation of a Rotor-Gene real-time PCR assay for detection, identification and quantification of C. trachomatis in a single reaction. J Clin Microbiol 44, 206–213.[CrossRef]
    [Google Scholar]
  10. Jespersen, D. J., Flatten, K. S., Jones, M. F. & Smith, T. F. ( 2005; ). Prospective comparison of cell cultures and nucleic acid amplification tests for laboratory diagnosis of Chlamydia trachomatis infections. J Clin Microbiol 43, 5324–5326.[CrossRef]
    [Google Scholar]
  11. Martin, D. H., Nsuami, M., Schachter, J., Hook, E. W., III, Ferrero, D., Quinn, T. C. & Gaydos, C. ( 2004; ). Use of multiple nucleic acid amplification tests to define the infected-patient “gold standard” in clinical trials of new diagnostic tests for Chlamydia trachomatis infections. J Clin Microbiol 42, 4749–4758.[CrossRef]
    [Google Scholar]
  12. Østergaard, L. ( 1999; ). Diagnosis of urogenital Chlamydia trachomatis infection by use of DNA amplification. APMIS 89, 5–36.
    [Google Scholar]
  13. Van Dyck, E., Ieven, M., Pattyn, S. L. V., Damme, L. V. & Laga, M. ( 2001; ). Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by enzyme immunoassay, culture, and three nucleic acid amplification tests. J Clin Microbiol 39, 1751–1756.[CrossRef]
    [Google Scholar]
  14. Vincelette, J., Schirm, J., Bogard, M., Bourgault, A. M., Luijt, D. S., Bianchi, A., van Voorst Vader, P. C., Butcher, A. & Rosenstraus, M. ( 1999; ). Multicenter evaluation of the fully automated COBAS Amplicor PCR test for detection of Chlamydia trachomatis in urogenital specimens. J Clin Microbiol 37, 74–80.
    [Google Scholar]
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