1887

Abstract

The development of rapid and sensitive molecular techniques for the detection of species would be useful for the surveillance of sporadic infections and management of major outbreaks. Comparative sequence analysis of the gene in the predominant species that cause human disease revealed distinct alleles for each examined species, including , and . Light Upon eXtension (LUX) real-time PCR assays were developed to target these species-specific polymorphisms, and were successful in rapidly differentiating the major pathogenic species. Luminex liquid microsphere array technology was used to develop a comprehensive assay capable of simultaneously detecting , and . These assays permitted the identification of a presumptive isolate as , which was verified using additional molecular characterization.

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2007-01-01
2019-11-15
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