1887

Abstract

The antibiotic sensitivity and the serotype and molecular type (MT) distribution of 41 strains isolated from individual patients in Tawam Hospital, Al Ain, United Arab Emirates, were investigated. While all strains were sensitive to erythromycin (MIC 0.5–4 mg l), 35 isolates (85.4 %) exhibited resistance to ciprofloxacin (MIC 8–64 mg l). All resistant strains carried the Thr-86 to Ile mutation in the gyrase A () gene, as shown by mismatch amplification mutation assay (MAMA) and confirmed by sequencing. Based on the partial sequences of , resistant isolates carried 10 distinct alleles, eight of them representing new variants. Strains were assigned to 30 MTs based on the combined results of PFGE and PCR-RFLP typing. Eight of the 35 ciprofloxacin-resistant strains, isolated over a period of more than 1 year, represented the largest MT, also carrying the same allelic variant of the gene. These results show that the local incidence of fluoroquinolone resistance among is one of the highest reported worldwide. It was also demonstrated that stable MTs could persist for a relatively long time among the clonally unrelated antibiotic-resistant isolates of . The data also emphasize the need to replace fluoroquinolones as empirical therapy for diarrhoea of undiagnosed aetiology.

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2006-11-01
2019-10-17
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