1887

Abstract

An outbreak of Legionnaires' disease at a long-term care facility in Ontario, Canada from September to October 2005 resulted in the death of 23 residents and the illness of 112 other people. In response, molecular methods were developed to detect in clinical lung samples and to subtype isolates from clinical and environmental samples. The targeted genetic loci included -specific virulence determinants (, , and ) and core bacterial determinants (, and ). An established amplified fragment length polymorphism typing method provided the first indication of genetic relatedness between strains recovered from clinical samples and strains cultured from environmental samples taken from the outbreak site. These associations were verified using the European Working Group for Infections sequence-based typing protocol targeting the , , , , and loci. These molecular typing methods confirmed the outbreak source as a contaminated air conditioning cooling tower.

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2007-03-01
2019-11-21
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