1887

Abstract

Five strains of O1, one each from an Australian and a New Zealand tourist with gastrointestinal illness returning from an island resort in Fiji and the remaining three from water sources located in the same resort, were extensively characterized. Conventional phenotypic traits that are used for biotyping of O1 categorized all five strains as belonging to the El Tor biotype. Genetic screening of 11 regions that are associated with virulence in showed variable results. The absence of genes comprising seventh pandemic island-I (VSP-I) and VSP-II in all the strains indicated that these strains were very similar to the pre-seventh pandemic O1 El Tor strains. The genes were absent in all strains whereas and were present in four strains, indicating that the strains were non-toxigenic. Four strains carried a truncated CTX prophage. Although epidemiological and molecular studies suggested that these strains did not cause cholera amongst tourists at the resort, their similarity to pre-seventh pandemic strains, their prior association with gastrointestinal illness and their presence in the island resort setting warrant more attention.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.46734-0
2006-11-01
2019-12-13
Loading full text...

Full text loading...

/deliver/fulltext/jmm/55/11/1559.html?itemId=/content/journal/jmm/10.1099/jmm.0.46734-0&mimeType=html&fmt=ahah

References

  1. Chow, K. H., Ng, T. K., Yuen, K. Y. & Yam, W. C. ( 2001; ). Detection of RTX toxin gene in Vibrio cholerae by PCR. J Clin Microbiol 39, 2594–2597.[CrossRef]
    [Google Scholar]
  2. Davis, B. M., Kimsey, H. H., Chang, W. & Waldor, M. K. ( 1999; ). The Vibrio cholerae O139 Calcutta bacteriophage CTXΦ is infectious and encodes a novel repressor. J Bacteriol 181, 6779–6787.
    [Google Scholar]
  3. Dziejman, M., Balon, E., Boyd, D., Fraser, C. M., Heidelberg, J. F. & Mekalanos, J. J. ( 2002; ). Comparative genomic analysis of Vibrio cholerae: genes that correlate with cholera endemic and pandemic disease. Proc Natl Acad Sci U S A 99, 1556–1561.[CrossRef]
    [Google Scholar]
  4. Heidelberg, J. F., Eisen, J. A., Nelson, W. C. & 29 other authors ( 2000; ). DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae. Nature 406, 477–484.[CrossRef]
    [Google Scholar]
  5. Hoshino, K., Yamasaki, S., Mukhopadhyay, A. K., Chakraborty, S., Basu, A., Bhattacharya, S. K., Nair, G. B., Shimada, T. & Takeda, Y. ( 1998; ). Development and evaluation of a multiplex PCR assay for rapid detection of toxigenic Vibrio cholerae O1 and O139. FEMS Immunol Med Microbiol 20, 201–207.[CrossRef]
    [Google Scholar]
  6. Jonson, G., Holmgren, J. & Svennerholm, A.-M. ( 1991; ). Identification of a mannose-binding pilus on Vibrio cholerae El Tor. Microb Pathog 11, 433–441.[CrossRef]
    [Google Scholar]
  7. Kaper, J. B., Morris, J. G., Jr & Levine, M. M. ( 1995; ). Cholera. Clin Microbiol Rev 8, 48–86.
    [Google Scholar]
  8. Keasler, S. P. & Hall, R. H. ( 1993; ). Detecting and biotyping V. cholerae O1 with multiplex polymerase chain reaction. Lancet 341, 1661.[CrossRef]
    [Google Scholar]
  9. Kimsey, H. H., Nair, G. B., Ghosh, A. & Waldor, M. K. ( 1998; ). Diverse CTXΦ and evolution of new pathogenic Vibrio cholerae. Lancet 352, 457–458.
    [Google Scholar]
  10. Lin, W., Fullner, K. J., Clayton, R., Sexton, J. A., Rogers, M. B., Calia, K. E., Calderwood, S. B., Fraser, C. & Mekalanos, J. J. ( 1999; ). Identification of a Vibrio cholerae RTX toxin gene cluster that is tightly linked to the cholera toxin prophage. Proc Natl Acad Sci U S A 96, 1071–1076.[CrossRef]
    [Google Scholar]
  11. Morris, J. G., Jr, Picardi, J. L., Lieb, S., Lee, J. V., Roberts, A., Hood, M., Gunn, R. A. & Blake, P. A. ( 1984; ). Isolation of nontoxigenic Vibrio cholerae O Group 1 from a patient with severe gastrointestinal disease. J Clin Microbiol 19, 296–297.
    [Google Scholar]
  12. Nandi, S., Maiti, D., Shaha, A. & Bhadra, R. K. ( 2003; ). Genesis of variants of V. cholerae O1 biotype El Tor: role of the CTXΦ array and its position in the genome. Microbiology 149, 89–97.[CrossRef]
    [Google Scholar]
  13. Nusrin, S., Khan, G. Y., Bhuiyan, N. A. & 9 other authors ( 2004; ). Diverse ctx phages among toxigenic Vibrio cholerae O1 and O139 strains isolated between 1994 and 2002 in an area where cholera is endemic in Bangladesh. J Clin Microbiol 42, 5854–5856.[CrossRef]
    [Google Scholar]
  14. O'Shea, A. Y., Reen, F. J., Quirke, A. M. & Boyd, E. F. ( 2004; ). Evolutionary genetic analysis of the emergence of epidemic Vibrio cholerae isolates on the basis of comparative nucleotide sequence analysis and multilocus virulence gene profiles. J Clin Microbiol 42, 4657–4671.[CrossRef]
    [Google Scholar]
  15. Rodrigue, D. C., Popovic, T. & Wachsmuth, I. K. ( 1994; ). Nontoxigenic Vibrio cholerae O1 infections in the United States. In Vibrio Cholerae and Cholera: Molecular to Global Perspectives, pp. 69–76. Edited by I. K. Wachsmuth, P. A. Blake & O. Olsvik. Washington, DC: American Society for Microbiology.
  16. World Health Organization ( 1987; ). Manual for Laboratory Investigations of Acute Enteric Infections. Geneva: World Health Organization.
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.46734-0
Loading
/content/journal/jmm/10.1099/jmm.0.46734-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error