1887

Abstract

The aim of this study was to examine the frequency and predictors of colonization of the respiratory tract by metallo--lactamase (MBL)-producing Gram-negative bacteria in patients admitted to a newly established intensive care unit (ICU) of a tertiary care hospital. Specimens of tracheobronchial aspirates for microbiological studies were obtained every day for the first 3 days of the ICU stay and subsequently every third day for the rest of the ICU stay. PCR analysis and nucleotide sequencing were performed to identify bacteria that had MBL genes. Thirty-five patients (20 male, 15 female) were hospitalized during the initial 3 month period of functioning of the ICU. Colonization of the lower respiratory tract by Gram-negative bacteria was found in 29 of 35 patients (83 %) during the first 6–20 days (median 13 days) following admission to the ICU (13 patients with , ten with , three with , two with and one with ). Six of 29 patients (21 %) colonized with Gram-negative bacteria had -positive isolates; one of these patients developed clinical infection due to this micro-organism. Previous use of carbapenems (=0.01) or other -lactams (=0.03), as well as a stay in the ICU of >20 days (<0.001), were associated with colonization with -producing . In conclusion, colonization by Gram-negative bacteria of the respiratory tract of patients in this newly established ICU was common (83 %). Use of -lactams, including carbapenems, was associated with subsequent colonization of the respiratory tract with MBL-positive .

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2006-10-01
2019-11-18
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