1887

Abstract

infection in humans is routinely assessed by serological means. Here, the authors attempted to compare the response of different strains to serological tests and to evaluate the antigenic profiles of the RH and RH Ankara (TRH) strains with Western blotting. Anti- IgG antibodies of 72 patients were examined with the indirect immunofluorescence antibody (IFA) test, ELISA and Western blotting (WB) by using antigen from both strains. Antigenic variations between strains did not affect IFA and ELISA test results, but qualitative and quantitative differences between the WB patterns were observed. A number of bands with molecular masses varying between 17 and 105 kDa were detected in WB. Fourteen different bands were obtained with the assay performed with RH strain antigen. An additional four bands were observed with TRH strain antigen. Also, an 80 kDa band was observed to stain darker in the blot with TRH strain antigen, whereas with RH strain antigen 30 and 38 kDa bands were darker. The results showed that strain-specific polymorphism in tachyzoite antigens of different strains is important in the evaluation of WB but not in conventional serological analyses such as ELISA and IFA.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.46712-0
2006-10-01
2019-10-17
Loading full text...

Full text loading...

/deliver/fulltext/jmm/55/10/1333.html?itemId=/content/journal/jmm/10.1099/jmm.0.46712-0&mimeType=html&fmt=ahah

References

  1. Ajzenberg, D., Dumetre, A. & Darde, M. L. ( 2005; ). Multiplex PCR for typing strains of Toxoplasma gondii. J Clin Microbiol 43, 1940–1943.[CrossRef]
    [Google Scholar]
  2. Appleford, P. J. & Smith, J. E. ( 1997; ). Toxoplasma gondii: the growth characteristics of three virulent strains. Acta Trop 65, 97–104.[CrossRef]
    [Google Scholar]
  3. Appleford, P. J. & Smith, J. E. ( 2000; ). Strain and stage specific variation in Toxoplasma gondii antigens. Int J Parasitol 30, 1187–1191.[CrossRef]
    [Google Scholar]
  4. Boothroyd, J. C. & Grigg, M. E. ( 2002; ). Population biology of Toxoplasma gondii and its relevance to human infection: do different strains cause different disease? Curr Opin Microbiol 5, 438–442.[CrossRef]
    [Google Scholar]
  5. Cazabonne, P., Bessieres, M. H. & Seguela, J. P. ( 1994; ). Kinetics study and characterisation of target excreted/secreted antigens of immunoglobulin G, M, A and E antibodies from mice infected with different strains of Toxoplasma gondii. Parasitol Res 80, 58–63.[CrossRef]
    [Google Scholar]
  6. Darde, M. L. ( 2004; ). Genetic analysis of the diversity in Toxoplasma gondii. Ann Ist Super Sanita 40, 57–63.
    [Google Scholar]
  7. Darde, M. L., Bouteille, B. & Pestre-Alexandre, M. ( 1992; ). Isoenzyme analysis of 35 Toxoplasma gondii isolates and the biological and epidemiological implications. J Parasitol 78, 786–794.[CrossRef]
    [Google Scholar]
  8. Degerli, K., Kilimcioglu, A. A., Kurt, O., Tamay, A. T. & Ozbilgin, A. ( 2003; ). Efficacy of azithromycin in a murine toxoplasmosis model, employing a Toxoplasma gondii strain from Turkey. Acta Trop 88, 45–50.[CrossRef]
    [Google Scholar]
  9. Doskaya, M., Degirmenci, A., Cicek, C., Ak, M., Korkmaz, M., Guruz, Y. & Uner, A. ( 2006; ). Behaviour of Toxoplasma gondii RH Ankara strain tachyzoites during continuous production in various cell lines. Parasitology 132, 315–319.[CrossRef]
    [Google Scholar]
  10. Dubey, J. P. ( 1998; ). Toxoplasmosis. In Topley and Wilson's Microbiology and Microbial Infections, pp. 303–318. Edited by F. E. G. Cox, J. P. Kreier & D. Wakelin. New York: Oxford University Press.
  11. Ertug, S., Uner, A., Aksoy, U., Gunduz, C. & Guruz, Y. ( 2000a; ). Correlation between ELISA, IFA and IHA techniques in the diagnosis of acute toxoplasmosis. Acta Parasitologica Turcica 24, 4–8.
    [Google Scholar]
  12. Ertug, S., Uner, A., Altıntas, N., Yolasıgmaz, A. & Petersen, E. ( 2000b; ). Comparison of ELISA and Western blotting methods for the detection of IgG antibodies against Toxoplasma gondii. Acta Parasitologica Turcica 24, 17–21.
    [Google Scholar]
  13. Hill, D. E., Chirukandoth, S. & Dubey, J. P. ( 2005; ). Biology and epidemiology of Toxoplasma gondii in man and animals. Anim Health Res Rev 6, 41–61.[CrossRef]
    [Google Scholar]
  14. Howe, D. K. & Sibley, L. D. ( 1995; ). Toxoplasma gondii comprises three clonal lineages: correlation of parasite genotype with human disease. J Infect Dis 172, 1561–1566.[CrossRef]
    [Google Scholar]
  15. Kong, J. T., Grigg, M. E., Uyetake, L., Parmley, S. & Boothroyd, J. C. ( 2003; ). Serotyping of Toxoplasma gondii infections in humans using synthetic peptides. J Infect Dis 187, 1484–1495.[CrossRef]
    [Google Scholar]
  16. Montoya, J. G. & Liesenfeld, O. ( 2004; ). Toxoplasmosis. Lancet 363, 1965–1976.[CrossRef]
    [Google Scholar]
  17. Rodgers, L., Wang, X., Wen, X., Dunford, B., Miller, R. & Suzuki, Y. ( 2005; ). Strains of Toxoplasma gondii used for tachyzoite antigens to stimulate spleen cells of infected mice in vitro affect cytokine responses of the cells in the culture. Parasitol Res 97, 332–335.[CrossRef]
    [Google Scholar]
  18. Saeij, J. P., Boyle, J. P. & Boothroyd, J. C. ( 2005; ). Differences among the three major strains of Toxoplasma gondii and their specific interactions with the infected host. Trends Parasitol 21, 476–481.[CrossRef]
    [Google Scholar]
  19. Ware, P. L. & Kasper, L. H. ( 1987; ). Strain-specific antigens of Toxoplasma gondii. Infect Immun 55, 778–783.
    [Google Scholar]
  20. Weiss, L. M., Udem, S. A., Tanowitz, H. & Wittner, M. ( 1988; ). Western blot analysis of the antibody response of patients with AIDS and Toxoplasma encephalitis: Antigenic diversity among Toxoplasma strains. J Infect Dis 157, 7–13.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.46712-0
Loading
/content/journal/jmm/10.1099/jmm.0.46712-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error