1887

Abstract

HZ95 is an enterococcus with high-level resistance to aminoglycosides. Nine genes responsible for high-level aminoglycoside resistance, including (′)-(″)-, (″)-, (″)-, (″)-, (′)-, (′)-, (′)-, (′)- and (′)-, were not detected in HZ95. An 8 kb fragment from unconjugative plasmids of HZ95 was cloned, and expressed gentamicin resistance in DH5. The genetic structures (∼8 kb DNA fragment) containing these aminoglycoside-modifying enzyme genes in HZ95 were determined. The deduced amino acid sequence of the novel (″) allele, (″)-, had 93.7 % amino acid identity with APH(2″)-Id. The (″)- gene was bracketed upstream by an insertion sequence (IS)-like element and downstream by a streptomycin adenylyltransferase gene (). The streptomycin adenylyltransferase encoded by the gene had 80.3 % amino acid identity with the protein encoded by . The plasmid of ∼16 kb could hybridize with a PCR-generated (″)- intragenic probe. The IS-like element had 91 % identity with IS. IS, which commonly acts as a key factor in the dissemination of CTX-M-type -lactamase genes in Gram-negative bacteria, has not been reported in .

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.46702-0
2006-11-01
2019-10-24
Loading full text...

Full text loading...

/deliver/fulltext/jmm/55/11/1521.html?itemId=/content/journal/jmm/10.1099/jmm.0.46702-0&mimeType=html&fmt=ahah

References

  1. Ahmed, A. M. & Shimamoto, T. A. ( 2004; ). Plasmid-encoded class 1 integron carrying sat, a putative phosphoserine phosphatase gene and aadA2 from enterotoxigenic Escherichia coli O159 isolated in Japan. FEMS Microbiol Lett 235, 243–248.[CrossRef]
    [Google Scholar]
  2. Cao, V., Lambert, T. & Courvalin, P. ( 2002; ). ColE1-like plasmid pIP843 of Klebsiella pneumoniae encoding extended-spectrum beta-lactamase CTX-M-17. Antimicrob Agents Chemother 46, 1212–1217.[CrossRef]
    [Google Scholar]
  3. Chow, J. W., Zervos, M. J., Lerner, S. A., Thal, L. A., Donabedian, S. M., Jaworski, D. D., Tsai, S., Shaw, K. J. & Clewell, D. B. ( 1997; ). A novel gentamicin resistance gene in Enterococcus. Antimicrob Agents Chemother 41, 511–514.
    [Google Scholar]
  4. Clark, N. C., Olsvik, O., Swenson, J. M., Spiegel, C. A. & Tenover, F. C. ( 1999; ). Detection of a streptomycin/spectinomycin adenylyltransferase gene (aadA) in Enterococcus faecalis. Antimicrob Agents Chemother 43, 157–160.[CrossRef]
    [Google Scholar]
  5. CLSI ( 2005; ). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard. CLSI document M7–A6, 6th edn. Wayne, PA: Clinical and Laboratory Standards Institute.
  6. Derbise, A., Aubert, S. & Solh, N. E. ( 1997; ). Mapping the regions carrying three contiguous antibiotic resistance genes aadE, sat4, and aphA-3 in the genomes of staphylococci. Antimicrob Agents Chemother 41, 1024–1032.
    [Google Scholar]
  7. Donabedian, S. M., Thal, L. A., Hershberger, E. & 13 other authors ( 2003; ). Molecular characterization of gentamicin-resistant enterococci in the United States: Evidence of spread from animals to humans through food. J Clin Microbiol 41, 1109–1113.[CrossRef]
    [Google Scholar]
  8. Eliopoulos, G. M., Farber, B. F., Murray, B. E., Wennersten, C. & Moellering, R. C. ( 1984; ). Ribosomal resistance of clinical enterococcal isolates to streptomycin. Antimicrob Agents Chemother 25, 398–399.[CrossRef]
    [Google Scholar]
  9. Ferretti, J. J., Gilmore, K. S. & Courvalin, P. ( 1986; ). Nucleotide sequence analysis of the gene specifying the bifunctional 6′-aminoglycoside acetyltransferase enzyme in Streptococcus faecalis and identification and cloning of gene regions specifying the two activities. J Bacteriol 167, 631–638.
    [Google Scholar]
  10. Hallgren, A., Saeedi, B., Nilsson, M., Monstein, H. J., Isaksson, B., Hanberger, H. & Nilsson, L. E. ( 2003; ). Genetic relatedness among Enterococcus faecalis with transposon-mediated high-level gentamicin resistance in Swedish intensive care units. J Antimicrob Chemother 52, 162–167.[CrossRef]
    [Google Scholar]
  11. Ike, Y., Tanimoto, K., Tomita, H., Takeuchi, K. & Fujimoto, S. ( 1998; ). Efficient transfer of the pheromone-independent Enterococcus faecium plasmid pMG1 (Gmr) (65.1 kilobases) to Enterococcus strains during broth mating. J Bacteriol 180, 4886–4892.
    [Google Scholar]
  12. Kao, S. J., You, I., Clewell, D. B., Donabedian, S. M., Zervos, M. J., Petrin, J., Shaw, K. J. & Chowl, J. W. ( 2000; ). Detection of the high-level aminoglycoside resistance gene aph(2″)-Ib in Enterococcus faecium. Antimicrob Agents Chemother 44, 2876–2879.[CrossRef]
    [Google Scholar]
  13. Murray, B. E. ( 1998; ). Diversity among multidrug-resistant enterococci. Emerg Infect Dis 4, 37–47.[CrossRef]
    [Google Scholar]
  14. Perreten, V., Schwarz, F. V., Teuber, M. & Levy, S. B. ( 2001; ). Mdt(A), a new efflux protein conferring multiple antibiotic resistance in Lactococcus lactis and Escherichia coli. Antimicrob Agents Chemother 45, 1109–1114.[CrossRef]
    [Google Scholar]
  15. Poirel, L., Lartigue, M. F., Decousser, J. W. & Nordmann, P. ( 2005; ). ISEcp1B-mediated transposition of bla CTX-M in Escherichia coli. Antimicrob Agents Chemother 49, 447–450.[CrossRef]
    [Google Scholar]
  16. Projan, S. J., Moghazeh, S. & Novick, R. P. ( 1988; ). Nucleotide sequence of pS194, a streptomycin-resistance plasmid from Staphylococcus aureus. Nucleic Acids Res 16, 2179–2187.[CrossRef]
    [Google Scholar]
  17. Schaberg, D. R., Culver, D. H. & Gaynes, R. P. ( 1991; ). Major trends in the microbial etiology of nosocomial infection. Am J Med 91 (Suppl. 3B), 72S–75S.
    [Google Scholar]
  18. Schwarz, F. V., Perreten, V. & Teuber, M. ( 2001; ). Sequence of the 50-kb conjugative multiresistance plasmid pRE25 from Enterococcus faecalis RE25. Plasmid 46, 170–187.[CrossRef]
    [Google Scholar]
  19. Simjee, S., Fraise, A. P. & Gill, M. J. ( 1999; ). Plasmid heterogeneity and identification of a Tn5281-like element in clinical isolates of high-level gentamicin-resistant Enterococcus faecium isolated in the UK. J Antimicrob Chemother 43, 625–635.[CrossRef]
    [Google Scholar]
  20. Tai, S. F., Zervos, M. J., Clewell, D. B., Donabedian, S. M., Sahm, D. F. & Chow, J. W. ( 1998; ). A new high-level gentamicin resistance gene, aph(2″)-Id, in Enterococcus spp. Antimicrob Agents Chemother 42, 1229–1232.
    [Google Scholar]
  21. Wang, Q., Xu, Y. C., Wang, H., Chen, M. J., Zhang, X. Z., Xu, S. Z. & Wu, J. ( 1999; ). Antimicrobial resistant characteristics in enterococci and antibiotics for enterococcal infections. Chinese Journal of Laboratory Medicine 22, 154–156 (in Chinese).
    [Google Scholar]
  22. Werner, G., Hildebrandt, B. & Witte, W. ( 2001; ). Aminoglycoside-streptothricin resistance gene cluster aadE-sat4-aphA-3 disseminated among multiresistant isolates of Enterococcus faecium. Antimicrob Agents Chemother 45, 3267–3269.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.46702-0
Loading
/content/journal/jmm/10.1099/jmm.0.46702-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error