1887

Abstract

HZ95 is an enterococcus with high-level resistance to aminoglycosides. Nine genes responsible for high-level aminoglycoside resistance, including (′)-(″)-, (″)-, (″)-, (″)-, (′)-, (′)-, (′)-, (′)- and (′)-, were not detected in HZ95. An 8 kb fragment from unconjugative plasmids of HZ95 was cloned, and expressed gentamicin resistance in DH5. The genetic structures (∼8 kb DNA fragment) containing these aminoglycoside-modifying enzyme genes in HZ95 were determined. The deduced amino acid sequence of the novel (″) allele, (″)-, had 93.7 % amino acid identity with APH(2″)-Id. The (″)- gene was bracketed upstream by an insertion sequence (IS)-like element and downstream by a streptomycin adenylyltransferase gene (). The streptomycin adenylyltransferase encoded by the gene had 80.3 % amino acid identity with the protein encoded by . The plasmid of ∼16 kb could hybridize with a PCR-generated (″)- intragenic probe. The IS-like element had 91 % identity with IS. IS, which commonly acts as a key factor in the dissemination of CTX-M-type -lactamase genes in Gram-negative bacteria, has not been reported in .

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2006-11-01
2020-06-04
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