1887

Abstract

Acute gastroenteritis is a major source of morbidity and mortality among young children in developed and developing countries. Human adenoviruses (HAdVs), and in particular species F, are related to childhood diarrhoea worldwide. This study presents the results obtained during an investigation of HAdVs causing acute gastroenteritis in children hospitalized in Rio de Janeiro, RJ, Brazil, from April 1996 to September 2003, as well as in children with diarrhoea living in the slums of Salvador, BA, Brazil, from October 2001 to September 2003. A total of 3060 stool samples was analysed by an enzyme immunoassay for rotavirus and adenovirus (EIARA) and 61 (2 %) were found to be positive. HAdV presented with low prevalence throughout the year, with a slight but not significant increase in incidence in late summer and early autumn. Children up to 2 years of age were the most frequently affected (79 % of all positive samples). All positive samples were analysed further by generic and species-specific HAdV PCR protocols, confirming 100 % specificity of this rapid and inexpensive EIARA. Species F was the most prevalent (65 %), despite the occurrence of species A (12 %), C, D and co-infection F/D (5 % each) and species B and co-infections F/A, F/C and B/D (2 % each). In order to type the species F strains as HAdV-40 or -41, generic PCR and a fI restriction digest were performed. HAdV-40 and -41 were found to represent 62 % (23/37) and 38 % (14/37), respectively. These results demonstrated that a combination of generic and species-specific PCRs is useful and reliable for HAdV species and type identification directly from faecal specimens. The results confirmed the endemism of human adenoviruses, mainly species F, in children as aetiological agents of diarrhoea, although the limited sensitivity of EIARA as a screening method may have underestimated their prevalence.

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2007-03-01
2019-11-13
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References

  1. Adam, E., Nasz, I. & Lengyel, A. ( 1996; ). Characterization of adenovirus hexons by their epitope composition. Arch Virol 141, 1891–1907.[CrossRef]
    [Google Scholar]
  2. Akalu, A., Seidel, W., Liebermann, H., Bauer, U. & Döhner, L. ( 1998; ). Rapid identification of subgenera of human adenovirus by serological and PCR assays. J Virol Methods 71, 187–196.[CrossRef]
    [Google Scholar]
  3. Allard, A., Albinsson, B. & Wadell, G. ( 2001; ). Rapid typing of human adenoviruses by a general PCR combined with restriction endonuclease analysis. J Clin Microbiol 39, 498–505.[CrossRef]
    [Google Scholar]
  4. Amar, C. F. L., East, C., Maclure, E., McLauchlin, J., Jenkins, C., Duncanson, P. & Wareing, D. R. A. ( 2004; ). Blinded application of microscopy, bacteriological culture, immunoassays and PCR to detect gastrointestinal pathogens from faecal samples of patients with community-acquired diarrhoea. Eur J Clin Microbiol Infect Dis 23, 529–534.
    [Google Scholar]
  5. Barnes, G. L., Uren, E., Stevens, K. B. & Bishop, R. F. ( 1998; ). Etiology of acute gastroenteritis in hospitalized children in Melbourne, Australia, from April 1980 to March 1993. J Clin Microbiol 36, 133–138.
    [Google Scholar]
  6. Bereciartu, A., Bok, K. & Gómez, J. ( 2002; ). Identification of viral agents causing gastroenteritis among children in Buenos Aires, Argentina. J Clin Virol 25, 197–203.[CrossRef]
    [Google Scholar]
  7. Bon, F., Fascia, P., Dauvergne, M., Tenenbaum, D., Planson, H., Petion, A. M., Pothier, P. & Kohli, E. ( 1999; ). Prevalence of group A rotavirus, human calicivirus, astrovirus, and adenovirus type 40 and 41 infections among children with acute gastroenteritis in Dijon, France. J Clin Microbiol 37, 3055–3058.
    [Google Scholar]
  8. Brown, M. ( 1990; ). Laboratory identification of adenoviruses associated with gastroenteritis in Canada from 1983 to 1986. J Clin Microbiol 28, 1525–1529.
    [Google Scholar]
  9. Brown, M., Petric, M. & Middleton, P. J. ( 1984; ). Diagnosis of fastidious enteric adenoviruses 40 and 41 in stool specimens. J Clin Microbiol 20, 334–338.
    [Google Scholar]
  10. Chen, K.-T., Chen, P.-Y., Tang, R.-B., Huang, Y.-F., Lee, P.-I., Yang, J.-Y., Chen, H.-I., Bresee, J., Hummelman, E. & Glass, R. ( 2005; ). Sentinel hospital surveillance for rotavirus diarrhea in Taiwan, 2001–2003. J Infect Dis 192 (Suppl. 1), S44–S48.[CrossRef]
    [Google Scholar]
  11. de Jong, J. C., Bijlsma, K., Wermenbol, A. G., Verweij-Uijterwaal, M. W., van der Avoort, H. G. A. M., Wood, D. J., Bailey, A. S. & Osterhaus, A. D. M. E. ( 1993; ). Detection, typing, and subtyping of enteric adenoviruses 40 and 41 from fecal samples and observation of changing incidences of infections with these types and subtypes. J Clin Microbiol 31, 1562–1569.
    [Google Scholar]
  12. de Jong, J. C., Wermenbol, A. G., Verweij-Uijterwaal, M. W., Slaterus, K. W., Wertheim-Van Dillen, P., Van Doormum, G. J., Khoo, S. H. & Hierholzer, J. C. ( 1999; ). Adenoviruses from human immunodeficiency virus-infected individuals, including two strains that represent new candidate serotypes Ad50 and Ad51 of species B1 and D, respectively. J Clin Microbiol 37, 3940–3945.
    [Google Scholar]
  13. Dennehy, P. H., Nelson, S. M., Spangenberger, S., Noel, J. S., Monroe, S. S. & Glass, R. I. ( 2001; ). A prospective case-control study of the role of astrovirus in acute diarrhea among hospitalized young children. J Infect Dis 184, 10–15.[CrossRef]
    [Google Scholar]
  14. Gomes, S. A., Candeias, J. A., Monteiro, S. P., Pereira, H. G. & Niel, C. ( 1989; ). New genome types of adenovirus types 1, 3, and 5 isolated from stools of children in Brazil. J Clin Microbiol 27, 1022–1026.
    [Google Scholar]
  15. Hall, T. A. ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98.
    [Google Scholar]
  16. Harsi, C. M., Rolim, D. P., Gomes, S. A., Gilio, A. E., Stewien, K. E., Baldacci, E. R. & Candeias, J. A. ( 1995; ). Adenovirus genome types isolated from stools of children with gastroenteritis in Sao Paulo, Brazil. J Med Virol 45, 127–134.[CrossRef]
    [Google Scholar]
  17. Herrmann, J. E., Blacklow, N. R., Perron-Henry, D. M., Clements, E., Taylor, D. N. & Echeverria, P. ( 1988; ). Incidence of enteric adenovirus among children in Thailand and the significance of these viruses in gastroenteritis. J Clin Microbiol 26, 1783–1786.
    [Google Scholar]
  18. Hierholzer, J. ( 1995; ). Adenoviruses. In Diagnostic Procedures for Viral, Rickettsial and Chlamydial Infections, 7th edn, pp. 169–188. Edited by E. H. Lennette, D. A. Lennette & E. T. Lennette. Washington, DC: American Public Health Association.
  19. Horwitz, M. S. ( 2001; ). Adenoviruses. In Fields Virology, 4th edn, pp. 2301–2326. Edited by D. M. Knipe, M. Howley, D. E. Griffin, R. A. Lamb, M. A. Matin, B. Roizman & S. E. Straus. Philadelphia, PA: Lippincott Williams & Wilkins.
  20. Jarecki-Khan, K., Tzipori, S. R. & Unicomb, L. E. ( 1993; ). Enteric adenovirus infection among infants with diarrhea in rural Bangladesh. J Clin Microbiol 31, 484–489.
    [Google Scholar]
  21. Kim, K.-H., Yang, J.-M., Joo, S.-I., Cho, Y.-G., Glass, R. I. & Cho, Y.-J. ( 1990; ). Importance of rotavirus and adenovirus types 40 and 41 in acute gastroenteritis in Korean children. J Clin Microbiol 28, 2279–2284.
    [Google Scholar]
  22. Kosek, M., Bern, C. & Guerrant, R. L. ( 2003; ). The global burden of diarrhoeal disease, as estimated from studies published between 1992 and 2000. Bull World Health Organ 81, 197–204.
    [Google Scholar]
  23. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  24. Leite, J. P., Pereira, H. G., Azeredo, R. S. & Schatzmayr, H. G. ( 1985; ). Adenoviruses in faeces of children with acute gastroenteritis in Rio de Janeiro, Brazil. J Med Virol 15, 203–209.[CrossRef]
    [Google Scholar]
  25. Lew, J. F., Moe, C. L., Monroe, S. S., Allen, J. R., Harrison, B. M., Forrester, B. D., Stine, S. E., Woods, P. A., Hierholzer, J. C. & Herrmann, J. E. ( 1991; ). Astrovirus and adenovirus associated with diarrhea in children in day care settings. J Infect Dis 164, 673–678.[CrossRef]
    [Google Scholar]
  26. Linhares, A. L. ( 2002; ). Vacina contra rotavírus em Belém, Pará: bases epidemiológicas, inocuidade, imunogenicidade e eficácia, p. 199. Instituto Evandro Chagas, Fundação Nacional de Saúde.
  27. Liu, C., Grillner, L., Jonsson, K., Linde, A., Shen, K., Lindell, A. T., Wirgart, B. Z. & Johansen, K. ( 2006; ). Identification of viral agents associated with diarrhea in young children during a winter season in Beijing, China. J Clin Virol 35, 69–72.[CrossRef]
    [Google Scholar]
  28. Marie-Cardine, A., Gourlain, K., Mouterde, O., Castignolles, N., Hellot, M. F., Mallet, E. & Buffet-Janvresse, C. ( 2002; ). Epidemiology of acute viral gastroenteritis in children hospitalized in Rouen, France. Clin Infect Dis 34, 1170–1178.[CrossRef]
    [Google Scholar]
  29. McIver, C. J., Hansman, G., White, P., Doultree, J. C., Catton, M. & Rawlinson, W. D. ( 2001; ). Diagnosis of enteric pathogens in children with gastroenteritis. Pathology 33, 353–358.
    [Google Scholar]
  30. Oh, D.-Y., Gaedicke, G. & Schreier, E. ( 2003; ). Viral agents of acute gastroenteritis in German children: prevalence and molecular diversity. J Med Virol 71, 82–93.[CrossRef]
    [Google Scholar]
  31. Pereira, H. G., Azeredo, R. S., Leite, J. P., Andrade, Z. P. & De Castro, L. ( 1985; ). A combined enzyme immunoassay for rotavirus and adenovirus (EIARA). J Virol Methods 10, 21–28.[CrossRef]
    [Google Scholar]
  32. Pereira, H. G., Linhares, A. C., Candeias, J. A. & Glass, R. I. ( 1993; ). National laboratory surveillance of viral agents of gastroenteritis in Brazil. Bull Pan Am Health Organ 27, 224–233.
    [Google Scholar]
  33. Pring-Årkeblom, P., Trijssenaar, J. F. E., Adrian, T. & Hoyer, H. ( 1999; ). Multiplex polymerase chain reaction for subgenus-specific detection of human adenoviruses in clinical samples. J Med Virol 58, 87–92.[CrossRef]
    [Google Scholar]
  34. Rohayem, J., Berger, S., Juretzek, T., Herchenröder, O., Mogel, M., Poppe, M., Henker, J. & Rethwilm, A. ( 2004; ). A simple and rapid single-step muliplex PCR RT-PCR to detect Norovirus, Astrovirus and Adenovirus in clinical stool samples. J Virol Methods 118, 49–59.[CrossRef]
    [Google Scholar]
  35. Saderi, H., Roustai, M. H., Sabahi, F., Sadeghizadeh, M., Owlia, P. & de Jong, J. C. ( 2002; ). Incidence of enteric adenovirus gastroenteritis in Iranian children. J Clin Virol 24, 1–5.[CrossRef]
    [Google Scholar]
  36. Scaletsky, I. C., Pedroso, M. Z., Morais, M. B., Carvalho, R. L., Silva, R. M., Fabbricotti, S. H. & Fagundes-Neto, U. ( 1999; ). Association of patterns of Escherichia coli adherence to HEp-2 cells with acute and persistent diarrhea. Arq Gastroenterol 36, 54–60 (in Portuguese).
    [Google Scholar]
  37. Scott-Taylor, T., Ahluwalia, G., Klisko, B. & Hammond, G. W. ( 1990; ). Prevalent enteric adenovirus variant not detected by commercial monoclonal antibody enzyme immunoassay. J Clin Microbiol 28, 2797–2801.
    [Google Scholar]
  38. Soares, C. C., Volotão, E. M., Albuquerque, M. C., da Silva, F. M., de Carvalho, T. R., Nozawa, C. M., Linhares, R. E. & Santos, N. ( 2002; ). Prevalence of enteric adenoviruses among children with diarrhea in four Brazilian cities. J Clin Virol 23, 171–177.[CrossRef]
    [Google Scholar]
  39. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weigh matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  40. Uhnoo, I., Wadell, G., Svensson, L. & Johansson, M. E. ( 1984; ). Importance of enteric adenoviruses 40 and 41 in acute gastroenteritis in infants and young children. J Clin Microbiol 20, 365–372.
    [Google Scholar]
  41. Venacchio, L., Vezina, R. M., Mitchell, A. A., Lesko, S. M., Plaut, A. G. & Acheson, D. W. ( 2006; ). Diarrhea in American infants and young children in the community setting: incidence, clinical presentation and microbiology. Pediatr Infect Dis J 25, 2–7.[CrossRef]
    [Google Scholar]
  42. Wadell, G., Hammarskjold, M. L., Winberg, G., Varsanyi, T. M. & Sundell, G. ( 1980; ). Genetic variability of adenoviruses. Ann N Y Acad Sci 354, 16–42.[CrossRef]
    [Google Scholar]
  43. Wood, S. R., Sharp, I. R., Caul, E. O., Paul, I., Bailey, A. S., Hawkins, M., Pugh, S., Treharne, J. & Stevenson, S. ( 1997; ). Rapid detection and serotyping of adenovirus by direct immunofluorescence. J Med Virol 51, 198–201.[CrossRef]
    [Google Scholar]
  44. Xu, W., McDonough, M. C. & Erdman, D. D. ( 2000; ). Species-specific identification of human adenoviruses by a multiplex PCR assay. J Clin Microbiol 38, 4114–4120.
    [Google Scholar]
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