1887

Abstract

Between January and September 2003, 39 isolates of the family with phenotypically positive Vitek 1 extended-spectrum beta-lactamase (ESBL) test results were collected, originating from patients of two hospitals in Saxony, Germany. Plasmid DNA was isolated and screened by PCR for the presence of genes encoding beta-lactamases of SHV, TEM and CTX-M types. To differentiate ESBL and non-ESBL among SHV and TEM genes, detailed analysis of PCR products was performed. Twenty-four strains carried SHV-2, SHV-5 or SHV-12 genes. In a further 11 strains a CTX-M gene was detected. The CTX-M genes could be affiliated to the CTX-M-1 and CTX-M-9 cluster by RFLP analysis. In the case of four isolates, hyperproduction of the chromosomal beta-lactamase K1 was inferred, because genes of the above-mentioned types were not detected. The strains contained plasmid DNA between 45 and 160 kb in size. Common plasmid restriction patterns among SHV-5 producers provided evidence of horizontal spread. Twenty strains had a MIC for cefotaxime of ⩽4 mg l, 18 strains had the same MIC for ceftazidime, and nine strains had this MIC of >4 mg l for both antibiotics. The ESBL phenotypes often coincided with ciprofloxacin or gentamicin resistance.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.46670-0
2007-02-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/jmm/56/2/241.html?itemId=/content/journal/jmm/10.1099/jmm.0.46670-0&mimeType=html&fmt=ahah

References

  1. Alobwede, I., M'Zali, F. H., Livermore, D. M., Heritage, J., Todd, N. & Hawkey, P. M. ( 2003; ). CTX-M extended-spectrum β-lactamase arrives in the UK. J Antimicrob Chemother 51, 470–471.[CrossRef]
    [Google Scholar]
  2. Ambler, R. P., Coulson, A. F. W., Frère, J. M., Ghuysen, J.-M., Joris, B., Forsman, M., Levesque, R. C., Tiraby, G. & Waley, S. G. ( 1991; ). A standard numbering scheme for the class A β-lactamases. Biochem J 276, 269–272.
    [Google Scholar]
  3. Babini, G. S. & Livermore, D. M. ( 2000; ). Antimicrobial resistance amongst Klebsiella spp. collected from intensive care units in Western and Southern Europe in 1997–1998. J Antimicrob Chemother 45, 183–189.[CrossRef]
    [Google Scholar]
  4. Babini, G. S., Yuan, M., Hall, L. M. C. & Livermore, D. M. ( 2003; ). Variable susceptibility to piperacillin/tazobactam amongst Klebsiella spp. with extended-spectrum β-lactamases. J Antimicrob Chemother 51, 605–612.[CrossRef]
    [Google Scholar]
  5. Backman, A., Orvelid, P., Vazquez, J. A., Skold, O. & Olcen, P. ( 2000; ). Complete sequence of a beta-lactamase-encoding plasmid in Neisseria meningitidis. Antimicrob Agents Chemother 44, 210–212.[CrossRef]
    [Google Scholar]
  6. Bauernfeind, A., Grimm, H. & Schweighart, S. ( 1990; ). A new plasmidic cefotaximase in a clinical isolate of Escherichia coli. Infection 18, 294–298.[CrossRef]
    [Google Scholar]
  7. Bauernfeind, A., Rosenthal, E., Eberlein, E., Holley, M. & Schweighart, S. ( 1993; ). Spread of Klebsiella pneumoniae producing SHV-5 beta-lactamase among hospitalized patients. Infection 21, 18–22.[CrossRef]
    [Google Scholar]
  8. Bedenič, B., Randegger, C., Boras, A. & Hächler, H. ( 2001; ). Comparison of five different methods for detection of SHV extended-spectrum β-lactamases. J Chemother 13, 24–33.[CrossRef]
    [Google Scholar]
  9. Bonnet, R. ( 2004; ). Growing group of extended-spectrum beta-lactamases: the CTX-M enzymes. Antimicrob Agents Chemother 48, 1–14.[CrossRef]
    [Google Scholar]
  10. Bradford, P. A. ( 2001; ). Extended-spectrum β-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clin Microbiol Rev 14, 933–951.[CrossRef]
    [Google Scholar]
  11. Bush, K. ( 2001; ). New β-lactamases in Gram-negative bacteria: diversity and impact on the selection of antimicrobial therapy. Clin Infect Dis 32, 1085–1089.[CrossRef]
    [Google Scholar]
  12. Bush, K., Jacoby, G. A. & Medeiros, A. A. ( 1995; ). A functional classification scheme for beta-lactamases and its correlation with molecular structure. Antimicrob Agents Chemother 39, 1211–1233.[CrossRef]
    [Google Scholar]
  13. Chang, F.-Y., Siu, L. K., Fung, C.-P., Huang, M.-H. & Ho, M. ( 2001; ). Diversity of SHV and TEM β-lactamases in Klebsiella pneumoniae: gene evolution in Northern Taiwan and two novel β-lactamases, SHV-25 and SHV-26. Antimicrob Agents Chemother 45, 2407–2413.[CrossRef]
    [Google Scholar]
  14. Coque, T. M., Oliver, A., Perez-Diaz, J. C., Baquero, F. & Canton, R. ( 2002; ). Genes encoding TEM-4, SHV-2, and CTX-M-10 extended-spectrum beta-lactamases are carried by multiple Klebsiella pneumoniae clones in a single hospital (Madrid, 1989 to 2000). Antimicrob Agents Chemother 46, 500–510.[CrossRef]
    [Google Scholar]
  15. De Champs, C., Chanal, C., Sirot, D., Baraduc, R., Romaszko, J. P., Bonnet, R., Plaidy, A., Boyer, M., Carroy, E. & other authors ( 2004; ). Frequency and diversity of class A extended-spectrum beta-lactamases in hospitals of the Auvergne, France: a 2 year prospective study. J Antimicrob Chemother 54, 634–639.[CrossRef]
    [Google Scholar]
  16. Deutsches Institut für Normung ( 1989; ). Methoden zur Empfindlichkeitsprüfung von mikrobiellen Krankheitserregern (außer Mykobakterien) gegen Chemotherapeutika. Teil 6: Bestimmung der minimalen Hemmkonzentration nach der Agar-Dilutionsmethode. DIN-58940–6. Berlin: Beuth-Verlag.
  17. Domenico, P., Marx, J. L., Schoch, P. E. & Cunha, B. A. ( 1992; ). Rapid plasmid DNA isolation from mucoid Gram-negative bacteria. J Clin Microbiol 30, 2859–2863.
    [Google Scholar]
  18. Edelstein, M., Pimkin, M., Palagin, I., Edelstein, I. & Stratchounski, L. ( 2003; ). Prevalence and molecular epidemiology of CTX-M extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae in Russian hospitals. Antimicrob Agents Chemother 47, 3724–3732.[CrossRef]
    [Google Scholar]
  19. Elwell, L. P. & Falkow, S. ( 1986; ). The characterization of R plasmids and the detection of plasmid-specified genes. In Antibiotics in Labaratory Medicine, 2nd edn, pp. 683–721. Edited by V. Lorian. Baltimore: Williams and Wilkins.
  20. Fournier, B., Lu, C. Y., Lagrange, P. H., Krishnamoorthy, R. & Phillippon, A. ( 1995; ). Point mutation in the Pribnow-box, the molecular basis of β-lactamase overproduction in Klebsiella oxytoca. Antimicrob Agents Chemother 39, 1365–1368.[CrossRef]
    [Google Scholar]
  21. Geiss, H. K., Mack, D. & Seifert, H. ( 2003; ). Konsensuspapier zur Identifizierung von speziellen Resistenzmechanismen und zur Interpretation von Ergebnissen der Antibiotikaempfindlichkeitstestung bei grampositiven und gramnegativen Erregern. Der Mikrobiologe 13, 222–239 (in German).
    [Google Scholar]
  22. Gniadkowski, M. ( 2001; ). Evolution and epidemiology of extended-spectrum β-lactamases (ESBLs) and ESBL-producing microorganisms. Clin Microbiol Infect 7, 597–608.[CrossRef]
    [Google Scholar]
  23. Gniadkowski, M., Schneider, I., Jungwirth, R., Hryniewicz, W. & Bauernfeind, A. ( 1998a; ). Ceftazidime resistant Enterobacteriaceae isolates from three Polish hospitals: identification of three novel TEM- and SHV-5-type extended-spectrum β-lactamases. Antimicrob Agents Chemother 42, 514–520.
    [Google Scholar]
  24. Gniadkowski, M., Schneider, I., Palucha, A., Jungwirth, R., Mikiewicz, B. & Bauernfeind, A. ( 1998b; ). Cefotaxime-resistant Enterobacteriaceae isolates from a hospital in Warsaw, Poland: identification of a new CTX-M-3 cefotaxime-hydrolyzing beta-lactamase that is closely related to the CTX-M-1/MEN-1 enzyme. Antimicrob Agents Chemother 42, 827–832.
    [Google Scholar]
  25. Hall, T. A. ( 1999; ). BioEdit: a user-friendly biologic sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98.
    [Google Scholar]
  26. Heritage, J., M'Zali, F. H., Gascoyne-Binzi, D. & Hawkey, P. M. ( 1999; ). Evolution and spread of SHV extended-spectrum β-lactamases in Gram-negative bacteria. J Antimicrob Chemother 44, 309–318.[CrossRef]
    [Google Scholar]
  27. Howard, C., van Daal, A., Kelly, G., Schooneveldt, J., Nimmo, G. & Giffard, P. M. ( 2002; ). Identification and minisequencing-based discrimination of SHV β-lactamases in nosocomial infection-associated Klebsiella pneumoniae in Brisbane, Australia. Antimicrob Agents Chemother 46, 659–664.[CrossRef]
    [Google Scholar]
  28. Jacoby, G. A. & Medeiros, A. A. ( 1991; ). More extended-spectrum β-lactamases. Antimicrob Agents Chemother 35, 1697–1704.[CrossRef]
    [Google Scholar]
  29. Jeong, S. H., Kim, W. M., Chang, C. L., Kim, J. M., Lee, K., Chong, Y., Hwang, H. Y., Baek, Y. W., Chung, H. K. & other authors ( 2001; ). Neonatal intensive care unit outbreak caused by a strain of Klebsiella oxytoca resistant to aztreonam due to overproduction of chromosomal beta-lactamase. J Hosp Infect 48, 281–288.[CrossRef]
    [Google Scholar]
  30. Kim, Y.-K., Pai, H., Lee, H.-J., Park, S.-E., Choi, E.-H., Kim, J., Kim, J. H. & Kim, E. C. ( 2002; ). Bloodstream infections by extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae in children: epidemiology and clinical outcome. Antimicrob Agents Chemother 46, 1481–1491.[CrossRef]
    [Google Scholar]
  31. Kim, J., Lim, Y.-M., Rheem, I., Lee, Y., Lee, J. C., Seol, S. Y., Lee, Y. C. & Cho, D. T. ( 2005; ). CTX-M and SHV-12 beta-lactamases are the most common extended-spectrum enzymes in clinical isolates of Escherichia coli and Klebsiella pneumoniae collected from 3 university hospitals within Korea. FEMS Microbiol Lett 245, 93–98.[CrossRef]
    [Google Scholar]
  32. Kliebe, C., Nies, B. A., Meyer, J. F., Tolxdorff-Neutzling, R. M. & Wiedemann, B. ( 1985; ). Evolution of plasmid-coded resistance to broad-spectrum cephalosporins. Antimicrob Agents Chemother 28, 302–307.[CrossRef]
    [Google Scholar]
  33. Leverstein-van Hall, M. A., Fluit, A. C., Paauw, A., Box, A. T. A., Brisse, S. & Verhoef, J. ( 2002; ). Evaluation of the Etest ESBL and the BD Phoenix, VITEK 1, and VITEK 2 automated instruments for detection of extended-spectrum beta-lactamases in multiresistant Escherichia coli and Klebsiella spp. J Clin Microbiol 40, 3703–3711.[CrossRef]
    [Google Scholar]
  34. Liu, C. P., Wang, N. Y., Lee, C. M., Weng, L. C., Tseng, H. K., Liu, C. W., Chiang, C. S. & Huang, F. Y. ( 2004; ). Nosocomial and community-acquired Enterobacter cloacae bloodstream infection: risk factors for and prevalence of SHV-12 in multiresistant isolates in a medical centre. J Hosp Infect 58, 63–77.[CrossRef]
    [Google Scholar]
  35. Livermore, D. M. ( 1995; ). β-Lactamases in laboratory and clinical resistance. Clin Microbiol Rev 8, 557–584.
    [Google Scholar]
  36. Mammeri, H., Laurans, G., Eveillard, M., Castelain, S. & Eb, F. ( 2001; ). Coexistence of SHV-4- and TEM-24-producing Enterobacter aerogenes strains before a large outbreak of TEM-24-producing strains in a French hospital. J Clin Microbiol 39, 2184–2190.[CrossRef]
    [Google Scholar]
  37. Mulvey, M. R., Bryce, E., Boyd, D., Ofner-Agostini, M., Christianson, S., Simor, A. E. & Paton, S. ( 2004; ). Ambler class A extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella spp. in Canadian hospitals. Antimicrob Agents Chemother 48, 1204–1214.[CrossRef]
    [Google Scholar]
  38. Ndugulile, F., Jureen, R., Harthug, S., Urassa, W. & Langeland, N. ( 2005; ). Extended spectrum β-lactamases among Gram-negative bacteria of nosocomial origin from an intensive care unit of a tertiary health facility in Tanzania. BMC Infect Dis 5, 86.[CrossRef]
    [Google Scholar]
  39. Nüesch-Inderbinen, M., Hächler, H. & Kayser, F. H. ( 1996; ). Detection of genes coding for extended-spectrum SHV beta-lactamases in clinical isolates by a molecular genetic method, and comparison with the E test. Eur J Clin Microbiol Infect Dis 15, 398–402.[CrossRef]
    [Google Scholar]
  40. Nüesch-Inderbinen, M., Kayser, F. H. & Hächler, H. ( 1997; ). Survey and molecular genetics of SHV β-lactamases in Enterobacteriaceae in Switzerland: two novel enzymes, SHV-11 and SHV-12. Antimicrob Agents Chemother 41, 943–949.
    [Google Scholar]
  41. Paterson, D. L., Ko, W.-C., von Gottenberg, A., Casellas, J. M., Mulazimoglu, L., Klugman, K. P., Bonomo, R. A., Rice, L. B., McCormack, J. G. & Yu, V. L. ( 2001; ). Outcome of cephalosporin treatment for serious infections due to apparently susceptible organisms producing extended-spectrum β-lactamases: implications for the clinical microbiology laboratory. J Clin Microbiol 39, 2206–2212.[CrossRef]
    [Google Scholar]
  42. Paterson, D. L., Hujer, K. M., Hujer, A. M., Yeiser, B., Bonomo, M. D., Rice, L. B., Bonomo, R. A. & International Klebsiella Study Group ( 2003; ). Extended-spectrum beta-lactamases in Klebsiella pneumoniae bloodstream isolates from seven countries: dominance and widespread prevalence of SHV- and CTX-M-type beta-lactamases. Antimicrob Agents Chemother 47, 3554–3560.[CrossRef]
    [Google Scholar]
  43. Péduzzi, J., Barthélémy, M., Tiwar, K., Mattioni, D. & Labia, R. ( 1989; ). Structural features related to hydrolytic activity against ceftazidime of plasmid-mediated SHV-type CAZ-5 beta-lactamase. Antimicrob Agents Chemother 33, 2160–2163.[CrossRef]
    [Google Scholar]
  44. Sambrook, J., Fritsch, E. F. & Maniatis, T. ( 1989; ). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  45. Thomson, K. S. & Moland, E. S. ( 2001; ). Cefepime, piperacillin-tazobactam and the inoculum effect in tests with extended-spectrum beta-lactamase-producing Enterobacteriaceae. Antimicrob Agents Chemother 45, 3548–3554.[CrossRef]
    [Google Scholar]
  46. Witte, W. & Mielke, M. ( 2003; ). β-Laktamasen mit breitem Wirkungsspektrum: Grundlagen, Epidemiologie, Schlußfolgerungen für die Prävention. Bundesgesundheitsbl-Gesundheitsforsch-Gesundheitsschutz 46, 881–890 (in German).[CrossRef]
    [Google Scholar]
  47. Wu, T.-L., Siu, L. K., Su, L.-H., Lauderdale, T. L., Lin, F. M., Leu, H.-S., Lin, T.-Y. & Ho, M. ( 2001; ). Outer membrane protein change combined with co-existing TEM-1 and SHV-1 β-lactamases lead to false identification of ESBL-producing Klebsiella pneumoniae. J Antimicrob Chemother 47, 755–761.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.46670-0
Loading
/content/journal/jmm/10.1099/jmm.0.46670-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error