1887

Abstract

This research was planned to detect IgG and IgG subclasses in sera of patients with active cutaneous leishmaniasis (CL). Sera from 30 patients with active CL aged between 10 and 50 years and from 30 healthy controls aged between 8 and 50 years were included in the study. Levels of IgG and its subclasses were measured by a nephelometer. Levels of IgG, IgG1 and IgG3 in the CL patients were higher than in the controls. In addition, IgG and IgG1, and IgG and IgG3 levels showed a significant positive correlation. These results showed that IgG subclasses could possibly be used as a helpful diagnostic marker in CL.

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2006-10-01
2019-10-21
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References

  1. Abbas, A. K., Murphy, K. M. & Sher, A. ( 1996; ). Functional diversity of helper T lymphocytes. Nature 383, 787–793.[CrossRef]
    [Google Scholar]
  2. Aksoy, N., Ozbilge, H., Keles, S., Iriadam, M., Vural, H. & Akcay, F. ( 2004; ). A preliminary approach to the separation of Leishmania cell-surface antigens. J Sep Sci 27, 1011–1016.[CrossRef]
    [Google Scholar]
  3. Anam, K., Afrin, F., Banerjee, D., Pramanik, N., Guha, S. K., Goswami, R. P., Saha, S. K. & Ali, N. ( 1999a; ). Differential decline in Leishmania membrane antigen-specific immunoglobulin G (IgG), IgM, IgE, and IgG subclass antibodies in Indian kala-azar patients after chemotherapy. Infect Immun 67, 6663–6669.
    [Google Scholar]
  4. Anam, K., Afrin, F., Banerjee, D., Pramanik, N., Guha, S. K., Goswami, R. P., Gupta, P. N., Saha, S. K. & Ali, N. ( 1999b; ). Immunoglobulin subclass distribution and diagnostic value of Leishmania donovani antigen-specific immunoglobulin G3 in Indian kala-azar patients. Clin Diagn Lab Immunol 6, 231–235.
    [Google Scholar]
  5. Antonelli, L. R., Dutra, W. O., Almeida, R. P., Bacellar, O., Carvalho, E. M. & Gollob, K. J. ( 2005; ). Activated inflammatory T cells correlate with lesion size in human cutaneous leishmaniasis. Immunol Lett 101, 226–230.[CrossRef]
    [Google Scholar]
  6. Atta, A. M., D'Oliveira, A., Correa, J., Atta, M. L., Almeida, R. P. & Carvalho, E. M. ( 1998; ). Anti-leishmanial IgE antibodies: a marker of active disease in visceral leishmaniasis. Am J Trop Med Hyg 59, 426–430.
    [Google Scholar]
  7. da Matta, V. L., Hoshino-Shimizu, S., Dietze, R. & Corbett, C. E. ( 2000; ). Detection of specific antibody isotypes and subtypes before and after treatment of American visceral leishmaniasis. J Clin Lab Anal 14, 5–12.[CrossRef]
    [Google Scholar]
  8. Deplazes, P., Smith, N. C., Arnold, P., Lutz, H. & Eckert, J. ( 1995; ). Specific IgG1 and IgG2 antibody responses of dogs to Leishmania infantum and other parasites. Parasite Immunol 17, 451–458.[CrossRef]
    [Google Scholar]
  9. Galvao-Castro, B., Sa Ferreira, J. A., Marzochi, K. F., Marzochi, M. C., Coutinho, S. G. & Lambert, P. H. ( 1984; ). Polyclonal B cell activation, circulating immune complexes and autoimmunity in human american visceral leishmaniasis. Clin Exp Immunol 56, 58–66.
    [Google Scholar]
  10. Gurel, S., Ulukanlıgil, M. & Ozbilge, H. ( 2002; ). Cutaneous leishmaniasis in Sanliurfa: epidemiologic and clinical features of the last four years (1997-2000). Int J Dermatol 41, 32–37.[CrossRef]
    [Google Scholar]
  11. Gutierrez, Y., Salinas, G. H., Palma, G., Valderrama, L. B., Santrich, C. V. & Saravia, N. G. ( 1991; ). Correlation between histopathology, immune response, clinical presentation, and evolution in Leishmania braziliensis infection. Am J Trop Med Hyg 45, 281–289.
    [Google Scholar]
  12. Miles, S. A., Conrad, S. M., Alves, R. G., Jeronimo, S. M. & Mosser, D. M. ( 2005; ). A role for IgG immune complexes during infection with the intracellular pathogen Leishmania. J Exp Med 201, 747–754.[CrossRef]
    [Google Scholar]
  13. Ravindran, R., Anam, K., Bairagi, B. C., Saha, B., Pramanik, N., Guha, S. K., Goswami, R. P., Banerjee, D. & Ali, N. ( 2004; ). Characterization of immunoglobulin G and its subclass response to Indian kala-azar infection before and after chemotherapy. Infect Immun 72, 863–870.[CrossRef]
    [Google Scholar]
  14. Reiner, S. L. & Locksley, R. M. ( 1995; ). The regulation of immunity to Leishmania major. Annu Rev Immunol 13, 151–177.[CrossRef]
    [Google Scholar]
  15. Rothman, P. & Coffman, R. L. ( 1996; ). Immunoglobulin heavy chain class-switching. In Weir's Handbook of Experimental Immunology, 5th edn, pp. 191–194. Edited by L. A. Herzenberg. Oxford: Blackwell Scientific Publications.
  16. Ryan, J. R., Smithyman, A. M., Rajasekariah, G. H., Hochberg, L., Stiteler, J. M. & Martin, S. K. ( 2002; ). Enzyme-linked immunosorbent assay based on soluble promastigote antigen detects immunoglobulin M (IgM) and IgG antibodies in sera from cases of visceral and cutaneous leishmaniasis. J Clin Microbiol 40, 1037–1043.[CrossRef]
    [Google Scholar]
  17. Skeiky, Y. A., Benson, D. R., Costa, J. L., Badaro, R. & Reed, S. G. ( 1997; ). Association of Leishmania heat shock protein 83 antigen and immunoglobulin G4 antibody titers in Brazilian patients with diffuse cutaneous leishmaniasis. Infect Immun 65, 5368–5370.
    [Google Scholar]
  18. Trujillo, C., Ramirez, R., Velez, I. D. & Berberich, C. ( 1999; ). The humoral immune response to the kinetoplastid membrane protein-11 in patients with American leishmaniasis and Chagas disease: prevalence of IgG subclasses and mapping of epitopes. Immunol Lett 70, 203–209.
    [Google Scholar]
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