@article{mbs:/content/journal/jmm/10.1099/jmm.0.46655-0, author = "Ramachandran, Dhanya and Bhanumathi, R. and Singh, Durg V.", title = "Multiplex PCR for detection of antibiotic resistance genes and the SXT element: application in the characterization of Vibrio cholerae", journal= "Journal of Medical Microbiology", year = "2007", volume = "56", number = "3", pages = "346-351", doi = "https://doi.org/10.1099/jmm.0.46655-0", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.46655-0", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", keywords = "Fr, furazolidone", keywords = "Cm, chloramphenicol", keywords = "Sul, sulfamethoxazole", keywords = "Str, streptomycin", keywords = "Tmp, trimethoprim", abstract = "This study describes a multiplex PCR assay for the detection of antibiotic resistance genes and the SXT element in Vibrio cholerae. Conditions were optimized to amplify fragments of sulII (encoding sulfamethoxazole resistance), dfrA1 (O1-specific trimethoprim resistance), dfr18 (O139-specific trimethoprim resistance), strB (streptomycin B resistance) and the SXT element simultaneously in one PCR. This multiplex PCR was evaluated on 142 V. cholerae isolates and the results correlated with the phenotypic antibiotic data obtained using a disc diffusion assay and a colony blot assay. Thus this one-step PCR can be used as a simple, rapid and accurate method for identification of antibiotic resistance profiles and could be used for the surveillance of the spread of antibiotic resistance determinants in epidemiological and environmental studies.", }