1887

Abstract

This study describes a multiplex PCR assay for the detection of antibiotic resistance genes and the SXT element in . Conditions were optimized to amplify fragments of (encoding sulfamethoxazole resistance), (O1-specific trimethoprim resistance), (O139-specific trimethoprim resistance), (streptomycin B resistance) and the SXT element simultaneously in one PCR. This multiplex PCR was evaluated on 142 isolates and the results correlated with the phenotypic antibiotic data obtained using a disc diffusion assay and a colony blot assay. Thus this one-step PCR can be used as a simple, rapid and accurate method for identification of antibiotic resistance profiles and could be used for the surveillance of the spread of antibiotic resistance determinants in epidemiological and environmental studies.

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2007-03-01
2019-11-22
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