@article{mbs:/content/journal/jmm/10.1099/jmm.0.46650-0, author = "Wang, Jang-Shiun and Yang, Yun-Liang and Wu, Chin-Jung and Ouyang, Karen J. and Tseng, Kuo-Yun and Chen, Chia-Geun and Wang, Hsin and Lo, Hsiu-Jung", title = "The DNA-binding domain of CaNdt80p is required to activate CDR1 involved in drug resistance in Candida albicans", journal= "Journal of Medical Microbiology", year = "2006", volume = "55", number = "10", pages = "1403-1411", doi = "https://doi.org/10.1099/jmm.0.46650-0", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.46650-0", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", keywords = "β-Gal, β-galactosidase", abstract = "CaNdt80p, the Candida albicans homologue of the Saccharomyces cerevisiae transcription factor ScNdt80p, has been identified as a positive regulator of CDR1, which encodes an efflux pump involved in drug resistance in C. albicans. To investigate the involvement of the putative DNA-binding domain of CaNdt80p in drug resistance, chimeras of CaNdt80p and ScNdt80p were constructed. Interestingly, the DNA-binding domain of ScNdt80p could functionally complement that of CaNdt80p to activate CDR1p–lacZ in S. cerevisiae. Consistently, CaNdt80p containing a mutation in the DNA-binding domain failed to activate CDR1p–lacZ in S. cerevisiae. Furthermore, a copy of CaNDT80 with the same mutation also failed to complement the drug-sensitive phenotype caused by a null mutation in C. albicans. Thus, the DNA-binding domain of CaNdt80p is critical for its function in drug resistance in C. albicans.", }