1887

Abstract

CaNdt80p, the homologue of the transcription factor ScNdt80p, has been identified as a positive regulator of , which encodes an efflux pump involved in drug resistance in . To investigate the involvement of the putative DNA-binding domain of CaNdt80p in drug resistance, chimeras of CaNdt80p and ScNdt80p were constructed. Interestingly, the DNA-binding domain of ScNdt80p could functionally complement that of CaNdt80p to activate in . Consistently, CaNdt80p containing a mutation in the DNA-binding domain failed to activate in . Furthermore, a copy of with the same mutation also failed to complement the drug-sensitive phenotype caused by a null mutation in . Thus, the DNA-binding domain of CaNdt80p is critical for its function in drug resistance in .

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.46650-0
2006-10-01
2020-08-15
Loading full text...

Full text loading...

/deliver/fulltext/jmm/55/10/1403.html?itemId=/content/journal/jmm/10.1099/jmm.0.46650-0&mimeType=html&fmt=ahah

References

  1. Abi-Said D., Anaissie E., Uzun O., Raad I., Pinzcowski H., Vartivarian S. 1997; The epidemiology of hematogenous candidiasis caused by different Candida species. Clin Infect Dis 24:1122–1128 [CrossRef]
    [Google Scholar]
  2. Beck-Sague C., Jarvis W. R. 1993; Secular trends in the epidemiology of nosocomial fungal infections in the United States, 1980–1990. National Nosocomial Infections Surveillance System. J Infect Dis 167:1247–1251 [CrossRef]
    [Google Scholar]
  3. Chen Y. C., Chang S. C., Sun C. C., Yang L. S., Hsieh W. C., Luh K. T. 1997; Secular trends in the epidemiology of nosocomial fungal infections at a teaching hospital in Taiwan; 1981 to 1993 Infect Control Hosp Epidemiol 18:369–375 [CrossRef]
    [Google Scholar]
  4. Chen C. G., Yang Y. L., Shih H. I., Su C. L., Lo H. J. 2004; CaNdt80 is involved in drug resistance in Candida albicans by regulating CDR1 . Antimicrob Agents Chemother 48:4505–4512 [CrossRef]
    [Google Scholar]
  5. Christianson T. W., Sikorski R. S., Dante M., Shero J. H., Hieter P. 1992; Multifunctional yeast high-copy-number shuttle vectors. Gene 110:119–122 [CrossRef]
    [Google Scholar]
  6. Chu S., Herskowitz I. 1998; Gametogenesis in yeast is regulated by a transcriptional cascade dependent on Ndt80. Mol Cell 1:685–696 [CrossRef]
    [Google Scholar]
  7. Chu S., DeRisi J., Eisen M., Mulholland J., Botstein D., Brown P. O., Herskowitz I. 1998; The transcriptional program of sporulation in budding yeast. Science 282:699–705 [CrossRef]
    [Google Scholar]
  8. Coste A. T., Karababa M., Ischer F., Bille J., Sanglard D. 2004; TAC 1, transcriptional activator of CDR genes, is a new transcription factor involved in the regulation of Candida albicans ABC transporters CDR1 and CDR2 . Eukaryot Cell 3:1639–1652 [CrossRef]
    [Google Scholar]
  9. Edwards E. J. J. 1990; Candida species. In Principles and Practice of Infectious Diseases pp  1943–1958 Edited by Mandell G. L., Douglas R. G., Bennett J. E. London: Churchill-Livingstone;
    [Google Scholar]
  10. Gillum A. M., Tsay E. Y., Kirsch D. R. 1984; Isolation of the Candida albicans gene for orotidine-5′-phosphate decarboxylase by complementation of S. cerevisiae ura3 and E. coli pyrF mutations. Mol Gen Genet 198:179–182 [CrossRef]
    [Google Scholar]
  11. Hung C. C., Chen Y. C., Chang S. C., Luh K. T., Hsieh W. C. 1996; Nosocomial candidemia in a university hospital in Taiwan. J Formos Med Assoc 95:19–28
    [Google Scholar]
  12. Karababa M., Coste A. T., Rognon B., Bille J., Sanglard D. 2004; Comparison of gene expression profiles of Candida albicans azole-resistant clinical isolates and laboratory strains exposed to drugs inducing multidrug transporters. Antimicrob Agents Chemother 48:3064–3079 [CrossRef]
    [Google Scholar]
  13. Kofron M., Demel T., Xanthos J. & 7 other authors; 1999; Mesoderm induction in Xenopus is a zygotic event regulated by maternal VegT via TGFbeta growth factors. Development 126:5759–5770
    [Google Scholar]
  14. Kohler J. R., Fink G. R. 1996; Candida albicans strains heterozygous and homozygous for mutations in mitogen-activated protein kinase signaling components have defects in hyphal development. Proc Natl Acad Sci U S A 93:13223–13228 [CrossRef]
    [Google Scholar]
  15. Lopez-Ribot J. L., McAtee R. K., Lee L. N., Kirkpatrick W. R., White T. C., Sanglard D., Patterson T. F. 1998; Distinct patterns of gene expression associated with development of fluconazole resistance in serial Candida albicans isolates from human immunodeficiency virus-infected patients with oropharyngeal candidiasis. Antimicrob Agents Chemother 42:2932–2937
    [Google Scholar]
  16. Marger M. D., Saier M. H. Jr 1993; A major superfamily of transmembrane facilitators that catalyse uniport, symport and antiport. Trends Biochem Sci 18:13–20 [CrossRef]
    [Google Scholar]
  17. Michaelis S., Berkower C. 1995; Sequence comparison of yeast ATP-binding cassette proteins. Cold Spring Harb Symp Quant Biol 60:291–307 [CrossRef]
    [Google Scholar]
  18. Montano S. P., Cote M. L., Fingerman I., Pierce M., Vershon A. K., Georgiadis M. M. 2002; Crystal structure of the DNA-binding domain from Ndt80, a transcriptional activator required for meiosis in yeast. Proc Natl Acad Sci U S A 99:14041–14046 [CrossRef]
    [Google Scholar]
  19. Pak J., Segall J. 2002; Regulation of the premiddle and middle phases of expression of the NDT80 gene during sporulation of Saccharomyces cerevisiae . Mol Cell Biol 22:6417–6429 [CrossRef]
    [Google Scholar]
  20. Pfaller M. A., Jones R. N., Messer S. A., Edmond M. B., Wenzel R. P. 1998; National surveillance of nosocomial blood stream infection due to species of Candida other than Candida albicans : frequency of occurrence and antifungal susceptibility in the SCOPE Program. Diagn Microbiol Infect Dis 30:121–129 [CrossRef]
    [Google Scholar]
  21. Pfaller M. A., Jones R. N., Doern G. V., Sader H. S., Messer S. A., Houston A., Coffman S., Hollis R. J. 2000; Bloodstream infections due to Candida species: SENTRY antimicrobial surveillance program in North America and Latin America, 1997–1998. Antimicrob Agents Chemother 44:747–751 [CrossRef]
    [Google Scholar]
  22. Pfaller M. A., Diekema D. J., Messer S. A., Boyken L., Hollis R. J. 2003; Activities of fluconazole and voriconazole against 1,586 recent clinical isolates of Candida species determined by broth microdilution, disk diffusion, and Etest methods: report from the ARTEMIS Global Antifungal Susceptibility Program, 2001. J Clin Microbiol 41:1440–1446 [CrossRef]
    [Google Scholar]
  23. Sanglard D., Ischer F., Monod M., Bille J. 1996; Susceptibilities of Candida albicans multidrug transporter mutants to various antifungal agents and other metabolic inhibitors. Antimicrob Agents Chemother 40:2300–2305
    [Google Scholar]
  24. Schaller M., Bein M., Korting H. C., Baur S., Hamm G., Monod M., Beinhauer S., Hube B. 2003; The secreted aspartyl proteinases Sap1 and Sap2 cause tissue damage in an in vitro model of vaginal candidiasis based on reconstituted human vaginal epithelium. Infect Immun 71:3227–3234 [CrossRef]
    [Google Scholar]
  25. Schmitt M. E., Brown T. A., Trumpower B. L. 1990; A rapid and simple method for preparation of RNA from Saccharomyces cerevisiae . Nucleic Acids Res 18:3091–3092 [CrossRef]
    [Google Scholar]
  26. Sherman F. 2002; Getting started with yeast. Methods Enzymol 350:3–41
    [Google Scholar]
  27. Tapia C., Leon E., Palavecino E. 2003; Antifungal susceptibility of yeasts by Etest. Comparison of 3 media. Rev Med Chil 131:299–302 (in Spanish
    [Google Scholar]
  28. Vanden Bossche H., Warnock D. W., Dupont B. & 7 other authors; 1994; Mechanisms and clinical impact of antifungal drug resistance. J Med Vet Mycol 32:189–202
    [Google Scholar]
  29. Yang Y. L., Lo H. J. 2001; Mechanisms of antifungal agent resistance. J Microbiol Immunol Infect 34:79–86
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.46650-0
Loading
/content/journal/jmm/10.1099/jmm.0.46650-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error