1887

Abstract

In this study a real-time PCR assay using self-quenched primers labelled with a single fluorophore for the detection of was developed. Probe specificity was confirmed by amplification of 16 strain templates and by the lack of a PCR product with 26 non- strains. With a pure culture of , the assay was linear over a range of 0.5 pg to 50 ng and was able to detect 16 c.f.u. per reaction. A similar sensitivity was observed in DNA extracted from a mixture of and fish tissue. Results using artificially inoculated tissues and diseased fish from outbreaks indicated that the assay can provide sensitive species-specific detection and quantification of in fish tissue.

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2007-03-01
2021-03-09
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