1887

Abstract

In this study, 250 vaginal samples from patients with vulvovaginal candidosis were inoculated onto two chromogenic media, Albicans ID and Biggy agar, as well as onto Sabouraud chloramphenicol agar, yielding a total of 63 yeast (25.2 %) on all three media. These strains were identified as in 20 (31.8 %) samples, in 15 samples (23.8 %), in 10 samples (15.9 %), in five samples (7.9 %), in five samples (7.9 %), in four samples (6.3 %), in two samples (3.2 %) and in two samples (3.2 %). Mixed fungal cultures and bacterial growth or filamentous fungi were not detected on any of the selected media. The sensitivity and specificity of the Albicans ID and Biggy agar with regard to the identification of were 80.0 and 64.6 %, and 86.7 and 56.3 %, respectively. This study showed these two chromogenic media to be as effective as Sabouraud chloramphenicol agar with respect to fungal detection. However, neither Albicans ID nor Biggy agar was sufficient for reliable differentiation of yeasts to the species level.

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2007-06-01
2019-11-16
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References

  1. Aktas, A. E., Al, F. D., Yigit, N. & Ayyildiz, A. ( 2001; ). Kromojenik bir besiyeri olan Biggy agarin Candida kökenlerinin tanimlandirilmasinda değerlendirilmesi. Turkiye Parazitol Derg 25, 301–304.
    [Google Scholar]
  2. Baumgartner, C., Freydiere, A. M. & Gille, Y. ( 1996; ). Direct identification and recognition of yeast species from clinical material by using Albicans ID and CHROMagar Candida plates. J Clin Microbiol 34, 454–456.
    [Google Scholar]
  3. Becker, H. & Schweisfurth, R. ( 1971; ). Der Laboratoriumtest: untersuchungen über ein Medium zu Diagnostik der vaginalen Candidosis. Mykosen 14, 127–130.
    [Google Scholar]
  4. Buckley, H. R. ( 1989; ). Identification of yeasts. In Medical Mycology: a Practical Approach, pp. 97–109. Edited by E. G. V. Evans & M. D. Richardson. Oxford: IRL Press.
  5. Cárdenes, C. D., Carrillo-Muñoz, A. J., Arias, A., Rodriguez-Alvarez, C., Torres-Lana, A., Sierra, A. & Arévalo, M. P. ( 2002; ). Comparison of Albicans ID2 agar plate with the germ tube for presumptive identification of Candida albicans. Diagn Microbiol Infect Dis 42, 181–185.[CrossRef]
    [Google Scholar]
  6. Cárdenes, C. D., Carrillo-Muñoz, A. J., Arias, A., Rodriguez-Alvarez, A., Torres-Lana, A., Sierra, A. & Arévalo, M. P. ( 2004; ). Comparative evaluation of four commercial tests for presumptive identification of Candida albicans. J Microbiol Methods 59, 293–297.[CrossRef]
    [Google Scholar]
  7. Contreras, I., San-Millán, R., Agustin-Barrasa, A., Pontón, J. & Quindós, G. ( 1996; ). Utility of Albicans ID plate for rapid identification of Candida albicans in clinical samples. Mycopathologia 136, 17–20.[CrossRef]
    [Google Scholar]
  8. De Champs, C., Lebeau, B., Ambroise-Thomas, P. & Grillot, R. ( 1995; ). Evaluation of Albicans ID plates. J Clin Microbiol 33, 2227–2228.
    [Google Scholar]
  9. Freydiére, A. M., Buchaille, L. & Gille, Y. ( 1997; ). Comparison of three commercial media for direct identification and discrimination of Candida species in clinical specimens. Eur J Clin Microbiol Infect Dis 16, 464–467.[CrossRef]
    [Google Scholar]
  10. Fricker-Hidalgo, H., Orenga, S., Lebeau, B., Pelloux, H., Brenier-Pinchart, M. P., Ambroise-Thomas, P. & Grillot, R. ( 2001; ). Evaluation of Candida ID, a new chromogenic medium for isolation and preliminary evaluation of some yeast species. J Clin Microbiol 39, 1647–1649.[CrossRef]
    [Google Scholar]
  11. Godoy, P., Almeida, L. P. & Colombo, A. L. ( 2001; ). Identification of Candida albicans using the chromogenic medium Albicans ID. Rev Iberoam Micol 18, 197–199.
    [Google Scholar]
  12. Hoppe, J. E. & Frey, P. ( 1999; ). Evaluation of six commercial tests and the germ-tube test for presumptive identification of Candida albicans. Eur J Clin Microbiol Infect Dis 18, 188–191.[CrossRef]
    [Google Scholar]
  13. Ilkit, M., Hilmioglu, S. & Cavusoglu, C. ( 1999; ). The evaluation of Albicans ID2, a new chromogenic medium for isolation and identification of Candida albicans. Turk J Infect 13, 161–163.
    [Google Scholar]
  14. Kalkanci, A., Kustimur, S. & Arslan, A. ( 1999; ). Çeşitli Candida kökenlerinin tiplendirilmesinde Biggy agar kullanilmasi. Infek Derg 13, 407–411.
    [Google Scholar]
  15. Lipperheide, V., Andraka, L., Pontón, J. & Quindós, G. ( 1993; ). Evaluation of Albicans ID plate method for the rapid identification of Candida albicans. Mycoses 36, 417–420.
    [Google Scholar]
  16. Mendel, E. B., Haberman, S. & Hall, D. K. ( 1960; ). Isolation of Candida from clinical specimens. Comparative study of Pagano–Levin and Nickerson’s culture media. Obstet Gynecol 16, 180–183.
    [Google Scholar]
  17. Nickerson, W. J. ( 1953; ). Reduction of inorganic substances by yeasts. I. Extracellular reduction of sulfite by species of Candida. J Infect Dis 93, 45–56.
    [Google Scholar]
  18. Niemann, D., Tintelnot, K. & Mendling, W. ( 2005; ). Vaginal carriage of Candida species – especially regarding Candida dubliniensis. Mycoses 48, (Suppl. 2), 73 [CrossRef]
    [Google Scholar]
  19. Odds, F. C. ( 1991; ). Sabouraud(’s) agar. J Med Vet Mycol 29, 355–359.[CrossRef]
    [Google Scholar]
  20. Odds, F. C. & Bernaerts, R. ( 1994; ). CHROMagar Candida, a new differential isolation medium for presumptive identification of clinically important yeast species. J Clin Microbiol 32, 1923–1929.
    [Google Scholar]
  21. Rousselle, P., Freydiére, A. M., Couillerot, P.-J., de Montclos, H. & Gille, Y. ( 1994; ). Rapid identification of Candida albicans by using Albicans ID and Fluoroplate agar plates. J Clin Microbiol 32, 3034–3036.
    [Google Scholar]
  22. Silva, J. O., Franceschini, S. A., Lavrador, M. A. S. & Candido, R. C. ( 2004; ). Performance of selective and differential media in the primary isolation of yeasts from different biological samples. Mycopathologia 157, 29–36.[CrossRef]
    [Google Scholar]
  23. Yucesoy, M. & Marol, S. ( 2003; ). Performance of CHROMagar Candida and Biggy agar for identification of yeast species. Ann Clin Microbiol Antimicrob 2, 8 [CrossRef]
    [Google Scholar]
  24. Yucesoy, M., Oztek, A. O. & Marol, S. ( 2005; ). Comparison of three different media for the presumptive identification of yeasts. Clin Microbiol Infect 11, 245–247.[CrossRef]
    [Google Scholar]
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