1887

Abstract

Recombinantly produced borrelial BBK32 protein fragments originating from , and were evaluated as antigens in the serology of Lyme borreliosis (LB). In ELISA, a mid-portion hydrophilic fragment reacted with LB patient sera. Of the 23 patients with culture- or PCR-positive erythema migrans (EM), 43 % at diagnosis and 52 % at convalescence were positive for at least one species-specific variant BBK32 fragment antigen. In parallel ELISAs with BBK32 whole proteins from the three borrelial subspecies as antigens, 17 % at diagnosis and 26 % at convalescence were positive. These results suggest that BBK32 protein fragments may improve the early IgG serology of LB compared to the BBK32 whole protein.

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2006-11-01
2024-12-06
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References

  1. Brown S. L., Hansen S. L., Langone J. J. 1999; Role of serology in the diagnosis of Lyme disease. JAMA 282:62–66 [CrossRef]
    [Google Scholar]
  2. Carlsson S.-A., Granlund H., Jansson C., Nyman D., Wahlberg P. 2003; Characteristics of erythema migrans in Borrelia afzelii and Borrelia garinii infections. Scand J Infect Dis 35:31–33 [CrossRef]
    [Google Scholar]
  3. Goettner G., Schulte-Spechtel U., Hillerman R., Liegl G., Wilske B., Fingerle V. 2005; Improvement of Lyme borreliosis serodiagnosis by a newly developed recombinant immunoglobulin G (IgG) and IgM line immunoblot assay and addition of VlsE and DbpA homologues. J Clin Microbiol 43:3602–3609 [CrossRef]
    [Google Scholar]
  4. Heikkilä T., Seppälä I., Saxen H., Panelius J., Peltomaa M., Julin T., Carlsson S. A., Lahdenne P. 2002; Recombinant BBK32 protein in serodiagnosis of early and late Lyme borreliosis. J Clin Microbiol 40:1174–1180 [CrossRef]
    [Google Scholar]
  5. Heikkilä T., Seppälä I., Saxen H., Panelius J., Yrjänäinen H., Lahdenne P. 2002; Species-specific serodiagnosis of Lyme arthritis and neuroborreliosis due to Borrelia burgdorferi sensu stricto, B. afzelii , and B. garinii by using decorin binding protein A. J Clin Microbiol 40:453–460 [CrossRef]
    [Google Scholar]
  6. Junttila J., Peltomaa M., Soini H., Marjamäki M., Viljanen M. K. 1999; Prevalence of Borrelia burgdorferi in Ixodes ricinus ticks in urban recreational areas of Helsinki. J Clin Microbiol 37:1361–1365
    [Google Scholar]
  7. Kim J. H., Singvall J., Schward-Linek U., Johnson B. J. B., Potts J. R., Höök M. 2004; BBK32, a fibronectin binding MSCRAMM from Borrelia burgdorferi , contains a disordered region that undergoes a conformational change on ligand binding. J Biol Chem 279:41706–41714 [CrossRef]
    [Google Scholar]
  8. Lahdenne P., Panelius J., Saxen H., Heikkilä T., Sillanpää H., Peltomaa M., Arnez M., Huppertz H.-I., Seppälä I. J. 2003; Improved serodiagnosis of erythema migrans using novel recombinant borrelial BBK32 antigens. J Med Microbiol 52:563–567 [CrossRef]
    [Google Scholar]
  9. Liang F. T., Steere A. C., Marques A. R., Johnson B. J. B., Miller J. N., Philipp M. T. 1999; Sensitive and specific serodiagnosis of Lyme disease by enzyme-linked immunosorbent assay with a peptide based on an immunodominant conserved region of Borrelia burgdorferi VlsE. J Clin Microbiol 37:3990–3996
    [Google Scholar]
  10. Magnarelli L. A., Ijdo J. W., Padula S. J., Flavell R. A., Fikrig E. 2000; Serologic diagnosis of Lyme borreliosis by using enzyme-linked immunosorbent assays with recombinant antigens. J Clin Microbiol 38:1735–1739
    [Google Scholar]
  11. Magnarelli L. A., Lawrenz M., Norris S. J., Fikrig E. 2002; Comparative reactivity of human sera to recombinant VlsE and other Borrelia burgdorferi antigens in class-specific enzyme-linked immunosorbent assays for Lyme borreliosis. J Med Microbiol 51:649–655
    [Google Scholar]
  12. Marangoni A., Sparacino M., Mondardini V., Cavrini F., Storni E., Donati M., Cevenini R., Sambri V. 2005; Comparative evaluation of two enzyme linked immunosorbent assay methods and three Western Blot methods for the diagnosis of culture-confirmed early Lyme borreliosis in Italy. New Microbiol 28:37–43
    [Google Scholar]
  13. Nadelman R. B., Wormser G. P. 1998; Lyme borreliosis. Lancet 352:557–565 [CrossRef]
    [Google Scholar]
  14. Probert W. S., Kim J. H., Höök M., Johnson B. J. B. 2001; Mapping the ligand-binding region of Borrelia burgdorferi fibronectin-binding protein BBK32. Infect Immun 69:4129–4133 [CrossRef]
    [Google Scholar]
  15. Robertson J., Guy E., Andrews N. & 9 other authors; 2000; A European multicenter study of immunoblotting in serodiagnosis of Lyme borreliosis. J Clin Microbiol 38:2097–2102
    [Google Scholar]
  16. Schulte-Spechtel U., Lehnert G., Liegl G., Fingerle V., Heimerl C., Johnson B. J. B., Wilske B. 2003; Significant improvement of the recombinant Borrelia -specific immunoglobulin G immunoblot test by addition of VlsE and a DbpA homologue derived from Borrelia garinii for diagnosis of early neuroborreliosis. J Clin Microbiol 41:1299–1303 [CrossRef]
    [Google Scholar]
  17. Seppälä I. J. T., Kroneld R., Schauman K., Forsen K. O., Lassenius R. 1994; Diagnosis of Lyme borreliosis: non-specific serological reactions with Borrelia burgdorferi sonicate antigen caused by IgG2 antibodies. J Med Microbiol 40:293–302 [CrossRef]
    [Google Scholar]
  18. Sigal L. H. 1998; Pitfalls in the diagnosis and management of Lyme disease. Arthritis Rheum 41:195–204 [CrossRef]
    [Google Scholar]
  19. Stromdahl E. Y., Williamson P. C., Kollars T. M. Jr, Evans S. R., Barry R. K., Vince M. A., Dobbs N. A. 2003; Evidence of Borrelia lonestari DNA in Amblyomma americanum (Acari: Ixodidae) removed from humans. J Clin Microbiol 41:5557–5562 [CrossRef]
    [Google Scholar]
  20. Wang G., van Dam A. P., Schwartz I., Dankert J. 1999; Molecular typing of Borrelia burgdorferi sensu lato: taxonomic, epidemiologic and clinical implications. Clin Microbiol Rev 12:633–653
    [Google Scholar]
  21. Wharton M., Chorba T. L., Vogt R. L., Morse D. L., Buehler J. W. 1990; Case definitions for public health surveillance. Morb Mortal Wkly Rep 39:19–21
    [Google Scholar]
  22. Wormser G. P., Nowakowski J., Nadelman R. B., Bittker S., Cooper D., Pavia C. 1998; Improving the yield of blood cultures for patients with early Lyme disease. J Clin Microbiol 36:296–298
    [Google Scholar]
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