1887

Abstract

Amplified fragment length polymorphism (AFLP) typing was applied to 116 isolates with different clinical backgrounds (invasive/pneumonia/carrier/human) and with known profiles of virulence-associated genes (1, -2, -7 and -9, as well as , and ). A dendrogram was generated that allowed identification of two clusters (A and C) with different subclusters (A1, A2, C1 and C2) and two heterogeneous groups of strains (B and D). For comparison, three strains from each AFLP subcluster and group were subjected to multilocus sequence typing (MLST) analysis. The closest relationship and lowest diversity were found for patterns clustering within AFLP subcluster A1, which corresponded with sequence type (ST) complex 1. Strains within subcluster A1 were mainly invasive 1 and + + (or *) + 2+ strains of porcine or human origin. A new finding of this study was the clustering of invasive * 9 isolates within subcluster A2. MLST analysis suggested that A2 correlates with a single ST complex (ST87). In contrast to A1 and A2, subclusters C1 and C2 contained mainly pneumonia isolates of genotype 7 or 2 and −. In conclusion, this study demonstrates that AFLP allows identification of clusters of strains with clinical relevance.

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2007-01-01
2019-11-12
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