1887

Abstract

In a previous study of isolates from cystic fibrosis (CF) patients in England and Wales, the Midlands 1 strain of was identified as the second most common clone, representing 10 % of isolates and found in nearly one-third of all CF centres [ Scott, F. W. & Pitt, T. L. (2004) . , 609–615]. Using suppression subtractive hybridization, 54 sequences were identified as present in a Midlands 1 strain but were absent from strain PAO1. The distribution of 14 of these sequences amongst representatives of Midlands 1, other CF epidemic strains and unrelated CF isolates was determined using PCR assays. Using these data, a PCR-based test was developed that was specific for the Midlands 1 clone, which was confirmed using dot-blot hybridization. By applying the test to CF isolates from a CF centre in Liverpool, a Midlands 1 clone was identified. The identity was confirmed using typing by PFGE. The PCR test should facilitate a greater understanding of the distribution of the Midlands 1 strain in the UK and elsewhere.

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2006-08-01
2019-10-15
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References

  1. Armstrong, D., Bell, S., Robinson, M. & 8 other authors ( 2003; ). Evidence for spread of a clonal strain of Pseudomonas aeruginosa among cystic fibrosis clinics. J Clin Microbiol 41, 2266–2267.[CrossRef]
    [Google Scholar]
  2. Arora, S. K., Bangera, M., Lory, S. & Ramphal, R. ( 2001; ). A genomic island in Pseudomonas aeruginosa carries the determinants of flagellin glycosylation. Proc Natl Acad Sci U S A 98, 9342–9347.[CrossRef]
    [Google Scholar]
  3. Arora, S. K., Wolfgang, M. C., Lory, S. & Ramphal, R. ( 2004; ). Sequence polymorphism in the glycosylation island and flagellins of Pseudomonas aeruginosa. J Bacteriol 186, 2115–2122.[CrossRef]
    [Google Scholar]
  4. Chambers, D., Scott, F., Bangur, R. & 7 other authors ( 2005; ). Factors associated with infection by Pseudomonas aeruginosa in adult cystic fibrosis. Eur Respir J 26, 651–656.[CrossRef]
    [Google Scholar]
  5. Cheng, K., Smyth, R. L., Govan, J. R. W., Doherty, C., Winstanley, C., Denning, N., Heaf, D. P., van Saene, H. & Hart, C. A. ( 1996; ). Spread of β-lactam-resistant Pseudomonas aeruginosa in a cystic fibrosis clinic. Lancet 348, 639–642.[CrossRef]
    [Google Scholar]
  6. Curran, B., Jonas, D., Grundmann, H., Pitt, T. & Dowson, C. G. ( 2004; ). Development of a multilocus sequence typing scheme for the opportunistic pathogen Pseudomonas aeruginosa. J Clin Microbiol 42, 5644–5649.[CrossRef]
    [Google Scholar]
  7. de Vos, D., de Chial, M., Cochez, C., Jansen, S., Tümmler, B., Meyer, J.-M. & Cornelis, P. ( 2001; ). Study of pyoverdine type and production by Pseudomonas aeruginosa isolated from cystic fibrosis patients: prevalence of type II pyoverdine isolates and accumulation of pyoverdine-negative mutations. Arch Microbiol 175, 384–388.[CrossRef]
    [Google Scholar]
  8. Ernst, R. K., D'Argenio, D. A., Ichikawa, J. K. & 12 other authors ( 2003; ). Genome mosaicism is conserved but not unique in Pseudomonas aeruginosa isolates from the airways of young children with cystic fibrosis. Environ Microbiol 5, 1341–1349.[CrossRef]
    [Google Scholar]
  9. Hill, D. F., Short, N. J., Perham, R. N. & Petersen, G. B. ( 1991; ). DNA sequence of the filamentous bacteriophage Pf1. J Mol Biol 218, 349–364.[CrossRef]
    [Google Scholar]
  10. Jones, A. M., Govan, J. R. W., Doherty, C. J., Dodd, M. E., Isalska, B. J., Stanbridge, T. N. & Webb, A. K. ( 2001; ). Spread of a multiresistant strain of Pseudomonas aeruginosa in an adult cystic fibrosis clinic. Lancet 358, 557–558.[CrossRef]
    [Google Scholar]
  11. Klockgether, J., Reva, O., Larbig, K. & Tümmler, B. ( 2004; ). Sequence analysis of the mobile genome island pKLC102 of Pseudomonas aeruginosa C. J Bacteriol 186, 518–534.[CrossRef]
    [Google Scholar]
  12. Larbig, K. D., Christmann, A., Johann, A., Klockgether, J., Hartsch, T., Merkl, R., Wiehlmann, L., Fritz, H.-J. & Tümmler, B. ( 2002; ). Gene islands integrated into tRNAGly genes confer genome diversity on a Pseudomonas aeruginosa clone. J Bacteriol 184, 6665–6680.[CrossRef]
    [Google Scholar]
  13. Lewis, D. A., Jones, A., Parkhill, J., Speert, D. P., Govan, J. R., Lipuma, J. J., Lory, S., Webb, A. K. & Mahenthiralingam, E. ( 2005; ). Identification of DNA markers for a transmissible Pseudomonas aeruginosa cystic fibrosis strain. Am J Respir Cell Mol Biol 33, 56–64.[CrossRef]
    [Google Scholar]
  14. O'Carroll, M. R., Syrmis, M. W., Wainwright, C. E., Greer, R. M., Mitchell, P., Coulter, C., Sloots, T. P., Nissen, M. D. & Bell, S. C. ( 2004; ). Clonal strains of Pseudomonas aeruginosa in paediatric and adult cystic fibrosis units. Eur Respir J 24, 101–106.[CrossRef]
    [Google Scholar]
  15. Ou, H.-Y., Smith, R., Lucchini, S., Hinton, J., Chaudhuri, R. R., Pallen, M., Barer, M. R. & Rajakumar, K. ( 2005; ). ArrayOme: a program for estimating the sizes of microarray-visualized bacterial genomes. Nucleic Acids Res 33, e3.[CrossRef]
    [Google Scholar]
  16. Panagea, S., Winstanley, C., Parsons, Y. N., Walshaw, M. J., Ledson, M. J. & Hart, C. A. ( 2003; ). PCR-based detection of a cystic fibrosis epidemic strain of Pseudomonas aeruginosa. Mol Diagn 7, 195–200.[CrossRef]
    [Google Scholar]
  17. Parsons, Y. N., Panagea, S., Smart, C. H. M., Walshaw, M. J., Hart, C. A. & Winstanley, C. ( 2002; ). Use of subtractive hybridization to identify a diagnostic probe for a cystic fibrosis epidemic strain of Pseudomonas aeruginosa. J Clin Microbiol 40, 4607–4611.[CrossRef]
    [Google Scholar]
  18. Pirnay, J.-P., de Vos, D., Cochez, C., Bilocq, F., Vanderkelen, A., Zizi, M., Ghysels, B. & Cornelis, P. ( 2002; ). Pseudomonas aeruginosa displays an epidemic population structure. Environ Microbiol 4, 898–911.[CrossRef]
    [Google Scholar]
  19. Rahme, L. G., Stevens, E. J., Wolfort, S. F., Shao, J., Tompkins, R. G. & Ausubel, F. M. ( 1995; ). Common virulence factors for bacterial pathogenicity in plants and animals. Science 268, 1899–1902.[CrossRef]
    [Google Scholar]
  20. Römling, U., Wingender, J., Müller, H. & Tümmler, B. ( 1994; ). A major Pseudomonas aeruginosa clone common to patients and aquatic habitats. Appl Environ Microbiol 60, 1734–1738.
    [Google Scholar]
  21. Salunkhe, P., Smart, C. H. M., Morgan, J. A. W., Panagea, S., Walshaw, M. J., Hart, C. A., Geffers, R., Tümmler, B. & Winstanley, C. ( 2005; ). A cystic fibrosis epidemic strain of Pseudomonas aeruginosa displays enhanced virulence and antimicrobial resistance. J Bacteriol 187, 4908–4920.[CrossRef]
    [Google Scholar]
  22. Scott, F. W. & Pitt, T. L. ( 2004; ). Identification and characterization of transmissible Pseudomonas aeruginosa strains in cystic fibrosis patients in England and Wales. J Med Microbiol 53, 609–615.[CrossRef]
    [Google Scholar]
  23. Smart, C. H. M., Walshaw, M. J., Hart, C. A. & Winstanley, C. ( 2006; ). Use of suppression subtractive hybridization to examine the accessory genome of the Liverpool cystic fibrosis epidemic strain of Pseudomonas aeruginosa. J Med Microbiol 55, 677–688.[CrossRef]
    [Google Scholar]
  24. Stover, C. K., Pham, X. Q., Erwin, A. L. & 28 other authors ( 2000; ). Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen. Nature 406, 959–964.[CrossRef]
    [Google Scholar]
  25. Tenover, F. C., Arbeit, R. D., Goering, R. V., Mickelsen, P. A., Murray, B. E., Persing, D. H. & Swaminathan, B. ( 1995; ). Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 33, 2233–2239.
    [Google Scholar]
  26. Winstanley, C. ( 2002; ). Spot the difference: applications of subtractive hybridization to the study of bacterial pathogens. J Med Microbiol 51, 459–467.
    [Google Scholar]
  27. Winstanley, C. & Hart, C. A. ( 2000; ). Presence of type III secretion genes in Burkholderia pseudomallei correlates with Ara phenotypes. J Clin Microbiol 38, 883–885.
    [Google Scholar]
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