1887

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Volume 55, Issue 12

influences macrophage uptake, cytokine expression and brain abscess formation in the neonatal rat Free

Abstract

causes neonatal meningitis frequently complicated with multiple brain abscesses. During central nervous system infection in the neonatal rat model, previous studies have documented many bacteria-filled macrophages within the neonatal rat brain and abscesses. Previous studies have also shown that is taken up by, survives phagolysosomal fusion and replicates in macrophages and . In this study, in order to elucidate genetic and cellular factors contributing to persistence, a combinatory technique of differential fluorescence induction and transposon mutagenesis was employed to isolate genes induced while inside macrophages. Several banks of mutants were subjected to a series of enrichments to select for  : : transposon fusion into genes that are turned off but expressed when intracellular within macrophages. Further screening identified several mutants attenuated in their recovery from macrophages compared with the wild-type. A mutation within an homologue caused significant attenuation in uptake and hypervirulence , resulting in death within 24 h. Furthermore, analysis of the immunoregulatory interleukin (IL)-10/IL-12 cytokine response during infection suggested that expression may alter this response. A better understanding of the bacteria–macrophage interaction at the molecular level and its contribution to brain abscess formation will assist in developing preventative and therapeutic strategies.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): CNS, central nervous system , CSF, cerebrospinal fluid , FACS, fluorescence activated cell sort , i.c., intracranial , i.p., intraperitoneal , IL, interleukin and PMA, phorbol 12-myristate 13-acetate
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2006-12-01
2024-04-19
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fliP influences Citrobacter koseri macrophage uptake, cytokine expression and brain abscess formation in the neonatal rat
J. Med. Microbiol. 55, 1631 (2006); https://doi.org/10.1099/jmm.0.46596-0
/content/journal/jmm/10.1099/jmm.0.46596-0
/content/journal/jmm/10.1099/jmm.0.46596-0
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