strain 905 reduces the translocation of serotype Typhimurium and stimulates the immune system in gnotobiotic and conventional mice Free

Abstract

Previous results in the laboratory of the authors showed that strain 905, isolated during ‘cachaça’ production, was able to colonize and survive in the gastrointestinal tract of germ-free and conventional mice, and to protect these animals against oral challenge with serotype Typhimurium or . In the present work, the effects of 905 on the translocation of . Typhimurium (mesenteric lymph nodes, Peyer's patches, spleen, liver) as well as on the immune system (number of Küpffer cells, immunoglobulin production, clearance of B) were evaluated in gnotobiotic and/or conventional mice. The treatment with the yeast reduced significantly the translocation of . Typhimurium to liver in gnotobiotic animals and to all the organs tested in conventional mice. The number of Küpffer cells per 100 hepatocytes in liver was significantly higher (<0.05) in yeast mono-associated mice (52.9±15.7) than in germ-free controls (38.1±9.0). Probably as a consequence, clearance of B from the bloodstream was more efficient in yeast mono-associated animals when compared to germ-free mice. Higher levels (<0.05) of secretory IgA in intestinal content and of IgA and IgM in serum were observed in yeast mono-associated mice when compared to germ-free group. Concluding, the protection against pathogenic bacteria observed in a previous study was probably due to a modulation of both local and systemic immunity of mice treated with 905.

Keyword(s): sIgA, secretory IgA
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2007-03-01
2024-03-29
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