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Volume 55, Issue 9

Typing of intimin () genes from enteropathogenic (EPEC) isolated from children with diarrhoea in Montevideo, Uruguay: identification of two novel intimin variants (B and R/2B) Free

Abstract

A total of 71 enteropathogenic (EPEC) strains isolated from children with diarrhoea in Montevideo, Uruguay, were characterized in this study. PCR showed that 57 isolates carried and genes (typical EPEC strains), and 14 possessed only the gene (atypical EPEC strains). These EPEC strains belonged to 21 O : H serotypes, including eight novel serotypes not previously reported among human EPEC in other studies. However, 72 % belonged to only four serotypes: O55 : H− (six strains), O111 : H2 (13 strains), O111 : H− (14 strains) and O119 : H6 (18 strains). Nine intimin types, namely, 1 (two O142 strains), 1 (29 strains, including 13 O111 : H2 and 14 O111 : H−), 1 (three O55 : H− strains), (five strains, including three strains with H40 antigen), (two strains), 1 (one strain), (one strain), B (six strains of serotypes O55 : H51 and O55 : H−) and R/2B (22 strains, including 18 O119 : H6) were detected among the 71 EPEC strains. The authors have identified two novel intimin genes (B and R/2B) in typical EPEC strains of serotypes O55 : H51/H− and O119 : H6/H−. The complete nucleotide sequences of the novel B and R/2 variant genes were determined. PFGE typing after I DNA digestion was performed on 44 representative EPEC strains. Genomic DNA fingerprinting revealed 44 distinct restriction patterns and the strains were clustered in 12 groups. Only 15 strains clustered in six groups of closely related (similarity >85 %) PFGE patterns, suggesting the prevailing clonal diversity among EPEC strains isolated from children with diarrhoea in Montevideo.

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Keyword(s): A/E, attaching and effacing , AEEC, attaching and effacing E. coli , DEC, diarrhoeagenic E. coli , EPEC, enteropathogenic E. coli , STEC, Shiga-toxin-producing E. coli and UPGMA, unweighted pair group method with arithmetic means
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2006-09-01
2024-04-18
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Typing of intimin (eae) genes from enteropathogenic Escherichia coli (EPEC) isolated from children with diarrhoea in Montevideo, Uruguay: identification of two novel intimin variants (μB and ξR/β2B)
J. Med. Microbiol. 55, 1165 (2006); https://doi.org/10.1099/jmm.0.46518-0
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