1887

Journal of Medical Microbiology logo

Volume 55, Issue 8

Increased prostanoid dependency of arterial relaxation in -infected mice Free

Abstract

Endothelial dysfunction plays an important role in the development of atherosclerosis. Previous studies have shown that inoculation with contributes to atherosclerotic development in rabbits and hypercholesterolaemic mice and causes endothelial dysfunction in apolipoprotein E-deficient mice. The effect of acute infection on endothelial function in normocholesterolaemic C57BL/6J mice was studied by measuring the force of contraction of the descending aorta after noradrenaline stimulation and in response to methacholine-induced relaxation. In addition, the effects of the nitric oxide synthase inhibitor -nitro--arginine methyl ester (-NAME) and the cyclooxygenase inhibitor diclofenac on relaxation were assessed. Pre-treatment of the aortas with -NAME decreased the relaxation response in both the infected and uninfected groups and no significant difference was detected between these groups, whereas diclofenac significantly attenuated the relaxation response only in the infected animals. In conclusion, infection shifted the balance of endothelium-derived relaxing factors from nitric oxide towards vasorelaxing prostanoids in C57BL/6J mice.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 6-keto PGF1α, 6-keto prostaglandin F1α , COX, cyclooxygenase , EDHF, endothelium-derived hyperpolarizing factor , GAPDH, glyceraldehyde-3-phosphate dehydrogenase , l-NAME, Nω-nitro-l-arginine methyl ester , MC, methacholine , NO, nitric oxide , NOS, nitric oxide synthase and PGI2, prostaglandin I2
SGM
Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.46516-0
2006-08-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/jmm/55/8/1017.html?itemId=/content/journal/jmm/10.1099/jmm.0.46516-0&mimeType=html&fmt=ahah

References

  1. Blessing E., Lin T.-M., Campbell L. A., Rosenfeld M. E., Lloyd D., Kuo C. 2000; Chlamydia pneumoniae induces inflammatory changes in the heart and aorta of normocholesterolemic C57BL/6J mice. Infect Immun 68:4765–4768 [CrossRef]
     [Google Scholar]
  2. Collins S., Martin T. L., Surwit R. S., Robidoux J. 2004; Genetic vulnerability to diet-induced obesity in the C57BL/6J mouse: physiological and molecular characteristics. Physiol Behav 81:243–248 [CrossRef]
     [Google Scholar]
  3. Erkkilä L., Laitinen K., Laurila A., Saikku P., Leinonen M. 2002; Experimental Chlamydia pneumoniae infection in NIH/S mice: effect of reinoculation with chlamydial or cell preparation on culture, PCR and histological findings of lung tissue. Vaccine 20:2318–2324 [CrossRef]
     [Google Scholar]
  4. Erkkilä L., Laitinen K., Haasio K., Tiirola T., Jauhiainen M., Lehr H. A., Aalto-Setälä K., Saikku P., Leinonen M. 2004; Heat shock protein 60 autoimmunity and early lipid lesions in cholesterol-fed C57BL/6JBom mice during Chlamydia pneumoniae infection. Atherosclerosis 177:321–328 [CrossRef]
     [Google Scholar]
  5. Feletou M., Vanhoutte P. M. 1999; The third pathway: endothelium-dependent hyperpolarization. J Physiol Pharmacol 50:525–534
     [Google Scholar]
  6. Hu H., Pierce G. N., Zhong G. 1999; The atherogenic effects of chlamydia are dependent on serum cholesterol and specific to Chlamydia pneumoniae . J Clin Invest 103:747–753 [CrossRef]
     [Google Scholar]
  7. Laskowitz D. T., Lee D. M., Schmechel D., Staats H. F. 2000; Altered immune responses in apolipoprotein E-deficient mice. J Lipid Res 41:613–620
     [Google Scholar]
  8. Leinonen M., Saikku P. 2002; Evidence for infectious agents in cardiovascular disease and atherosclerosis. Lancet Infect Dis 2:11–17 [CrossRef]
     [Google Scholar]
  9. Liuba P., Karnani P., Pesonen E., Paakkari I., Forslid A., Johansson L., Persson K., Wadström T., Laurini R. 2000; Endothelial dysfunction after repeated Chlamydia pneumoniae infection in apolipoprotein E-knockout mice. Circulation 102:1039–1044 [CrossRef]
     [Google Scholar]
  10. Liuba P., Pesonen E., Paakkari I. & 8 other authors; 2003; Co-infection with Chlamydia pneumoniae and Helicobacter pylori results in vascular endothelial dysfunction and enhanced VCAM-1 expression in apoE-knockout mice. J Vasc Res 40:115–122 [CrossRef]
     [Google Scholar]
  11. Moazed T. C., Kuo C., Grayston J. T., Campbell L. A. 1997; Murine models of Chlamydia pneumoniae infection and atherosclerosis. J Infect Dis 175:883–890 [CrossRef]
     [Google Scholar]
  12. Moazed T. C., Campbell L. A., Rosenfeld M. E., Grayston J. T., Kuo C. C. 1999; Chlamydia pneumoniae infection accelerates the progression of atherosclerosis in apolipoprotein E-deficient mice. J Infect Dis 180:238–241 [CrossRef]
     [Google Scholar]
  13. Paigen B., Morrow A., Brandon C., Mitchell D., Holmes P. 1985; Variation in susceptibility to atherosclerosis among inbred strains of mice. Atherosclerosis 57:65–73 [CrossRef]
     [Google Scholar]
  14. Ross R. 1999; Atherosclerosis – an inflammatory disease. N Engl J Med 340:115–126 [CrossRef]
     [Google Scholar]
  15. Rupp J., Berger M., Reiling N., Gieffers J., Lindschau C., Haller H., Dalhoff K., Maass M. 2004; Cox-2 inhibition abrogates Chlamydia pneumoniae -induced PGE2 and MMP-1 expression. Biochem Biophys Res Commun 320:738–744 [CrossRef]
     [Google Scholar]
  16. Simpson D. A. C., Feeney S., Boyle C., Stitt A. W. 2000; Retinal VEGF mRNA measured by SYBR green I fluorescence: a versatile approach to quantitative PCR. Mol Vis 6:178–183
     [Google Scholar]
  17. Tanigawa T., Watanabe T., Hamaguchi M. & 7 other authors; 2004; Anti-inflammatory effect of two isoforms of COX in H. pylori -induced gastritis in mice: possible involvement of PGE2 . Am J Physiol Gastrointest Liver Physiol 286:G148–G156
     [Google Scholar]
  18. Törmäkangas L., Erkkilä L., Korhonen T., Tiirola T., Bloigu A., Saikku P., Leinonen M. 2005a; Effects of repeated Chlamydia pneumoniae inoculations on aortic lipid accumulation and inflammatory response in C57BL/6J mice. Infect Immun 73:6458–6466 [CrossRef]
     [Google Scholar]
  19. Törmäkangas L., Vuorela P., Saario E., Leinonen M., Saikku P., Vuorela H. 2005b; In vivo treatment of acute Chlamydia pneumoniae infection with the flavonoids quercetin and luteolin and an alkyl gallate, octyl gallate, in a mouse model. Biochem Pharmacol 70:1222–1230 [CrossRef]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.46516-0
Loading
Increased prostanoid dependency of arterial relaxation in Chlamydia pneumoniae-infected mice
J. Med. Microbiol. 55, 1017 (2006); https://doi.org/10.1099/jmm.0.46516-0
/content/journal/jmm/10.1099/jmm.0.46516-0
/content/journal/jmm/10.1099/jmm.0.46516-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error