1887

Abstract

There are no data describing the genetic make-up of strains (an important aetiological agent of diarrhoea) circulating in the Arabian Gulf region. Here, the molecular characterization of two virulence genes in from Bahrain and the relationship with clinical infection are reported. Molecular screening for cytolethal distending toxin () and invasion-associated marker () genes was carried out on stool isolates collected from January 2002 to January 2004 in Bahrain. The molecular characterization was correlated with the patients' socio-demographic and clinical parameters. Of the 96 strains tested, 50 (52 %) were / , 30 (31 %) were / and 16 (17 %) were / . Sixty-nine per cent (66/96) of patients were less than 3 years old, with significantly higher detection of / and / strains ( <0.001 and <0.01, respectively) in this age group. Seventy patients (73 %) were symptomatic. In the group that were less than 3 years old, 62 and 85 % of those with / and / strains, respectively, were symptomatic compared with 100 % for those over 3 years of age. However, the presence of / strains still resulted in clinical infection in the children under 3 years but not in the older patients. This is the first report describing the molecular characterization of virulence genes in isolates from this region. The findings indicate that strains of different virulence genetic make-up are circulating in the population, with children under the age of 3 years being most vulnerable. Further work on the molecular characterization, gene expression and determination of the invasive phenotypes of strains circulating in different regions is needed.

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2006-07-01
2019-10-15
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