1887

Abstract

The ability of to feed on Gram-negative bacteria, as well as to harbour potential pathogens, such as , , , , , and , suggest that both amoebae and bacteria are involved in complex interactions, which may play important roles in the environment and in human health. In this study, (a keratitis isolate belonging to the T4 genotype) was used and its interactions with (strain K1, a cerebrospinal fluid isolate from a meningitis patient, O18 : K1 : H7, and a K-12 laboratory strain, HB101) were studied. The invasive K1 isolate exhibited a significantly higher association with than the non-invasive K-12 isolate. Similarly, K1 showed significantly increased invasion and/or uptake by in gentamicin protection assays than the non-invasive K-12. Using several mutants derived from K1, it was observed that outer-membrane protein A (OmpA) and LPS were crucial bacterial determinants responsible for K1 interactions with . Once inside the cell, K1 remained viable and multiplied within , while K-12 was killed. Again, OmpA and LPS were crucial for K1 intracellular survival in . In conclusion, these findings suggest that K1 interactions with are carefully regulated by the virulence of .

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2006-06-01
2019-10-21
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