1887

Abstract

is a novel Gram-negative bacterium of the subdivision recently found in the airways of patients with cystic fibrosis (CF). Here, the authors report on the clinical courses of six CF patients colonized with . Five patients suffered from either an acute respiratory exacerbation or a progressive loss of pulmonary function, whereas one patient was in a stable clinical situation. This study focused on two aims: (i) the clonal analysis of isolates by random amplified polymorphic DNA (RAPD)-PCR, and (ii) the clarification of whether the presence of in the respiratory tract is associated with a specific serum antibody response. Serum IgG was detected by immunoblotting using whole-cell-lysate proteins as antigens. Sera from healthy blood donors (=10) and from CF patients colonized with (=10) were found to be immunoblot negative. All six -positive patients raised serum IgG antibodies against various antigens. Surprisingly, in one patient, a specific serum antibody response was already detected 1 year prior to -positive sputum cultures. Two prominent antigens were characterized by MALDI-MS: a 23 kDa protein revealed homology to the outer membrane lipoprotein OmlA of , and an 18 kDa protein to a protein-tyrosine phosphatase of . In conclusion, detection of is accompanied by a specific serum antibody response and may reflect the infectious/pathogenic potential of . Moreover, IgG immunoblotting may be useful to detect early infection with and may support the selective cultivation of this novel emerging pathogen.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.46466-0
2006-10-01
2020-07-13
Loading full text...

Full text loading...

/deliver/fulltext/jmm/55/10/1425.html?itemId=/content/journal/jmm/10.1099/jmm.0.46466-0&mimeType=html&fmt=ahah

References

  1. Aronoff S. C., Quinn F. J. Jr, Stern R. C. 1991; Longitudinal serum IgG response to Pseudomonas cepacia surface antigens in cystic fibrosis. Pediatr Pulmonol 11:289–293 [CrossRef]
    [Google Scholar]
  2. Beringer P. M., Appleman M. D. 2000; Unusual respiratory bacterial flora in cystic fibrosis: microbiologic and clinical features. Curr Opin Pulm Med 6:545–550 [CrossRef]
    [Google Scholar]
  3. Burns J. L., Emerson J., Stapp J. R., Yim D. L., Krzewinski J., Louden L., Ramsey B. W., Clausen C. R. 1998; Microbiology of sputum from patients at cystic fibrosis centers in the United States. Clin Infect Dis 27:158–163 [CrossRef]
    [Google Scholar]
  4. Caballero E., Drobnic M. E., Perez M. T., Manresa J. M., Ferrer A., Orriols R. 2001; Anti- Pseudomonas aeruginosa antibody detection in patients with bronchiectasis without cystic fibrosis. Thorax 56:669–674 [CrossRef]
    [Google Scholar]
  5. Chiron R., Marchandin H., Counil F. & 8 other authors; 2005; Clinical and microbiological features of Inquilinus sp. isolates from five patients with cystic fibrosis. J Clin Microbiol 43:3938–3943 [CrossRef]
    [Google Scholar]
  6. Coenye T., Goris J., Spilker T., Vandamme P., LiPuma J. J. 2002a; Characterization of unusual bacteria isolated from respiratory secretions of cystic fibrosis patients and description of Inquilinus limosus gen. nov., sp. nov. J Clin Microbiol 40:2062–2069 [CrossRef]
    [Google Scholar]
  7. Coenye T., Vandamme P., LiPuma J. J. 2002b; Infection by Ralstonia species in cystic fibrosis patients: identification of R. pickettii and R. mannitolilytica by polymerase chain reaction. Emerg Infect Dis 8:692–696 [CrossRef]
    [Google Scholar]
  8. Fomsgaard A., Hoiby N., Shand G. H., Conrad R. S., Galanos C. 1988; Longitudinal study of antibody response to lipopolysaccharides during chronic Pseudomonas aeruginosa lung infection in cystic fibrosis. Infect Immun 56:2270–2278
    [Google Scholar]
  9. Gilligan P. H. 1991; Microbiology of airway disease in patients with cystic fibrosis. Clin Microbiol Rev 4:35–51
    [Google Scholar]
  10. Gram T., Ahrens P. 1998; Improved diagnostic PCR assay for Actinobacillus pleuropneumoniae based on the nucleotide sequence of an outer membrane lipoprotein. J Clin Microbiol 36:443–448
    [Google Scholar]
  11. Hendry J., Butler S., Elborn J. S., Govan J. R., Nelson J., Shale D. J., Webb A. K. 2000; Antibody response to Burkholderia cepacia in patients with cystic fibrosis colonized with Burkholderia cepacia and Pseudomonas aeruginosa . J Infect 40:164–170 [CrossRef]
    [Google Scholar]
  12. Hogardt M., Trebesius K., Geiger A. M., Hornef M., Rosenecker J., Heesemann J. 2000; Specific and rapid detection by fluorescent in situ hybridization of bacteria in clinical samples obtained from cystic fibrosis patients. J Clin Microbiol 38:818–825
    [Google Scholar]
  13. Johansen H. K., Norregaard L., Gotzsche P. C., Pressler T., Koch C., Hoiby N. 2004; Antibody response to Pseudomonas aeruginosa in cystic fibrosis patients: a marker of therapeutic success? – A 30-year cohort study of survival in Danish CF patients after onset of chronic P. aeruginosa lung infection. Pediatr Pulmonol 37:427–432 [CrossRef]
    [Google Scholar]
  14. Jorgensen I. M., Johansen H. K., Frederiksen B., Pressler T., Hansen A., Vandamme P., Hoiby N., Koch C. 2003; Epidemic spread of Pandoraea apista , a new pathogen causing severe lung disease in cystic fibrosis patients. Pediatr Pulmonol 36:439–446 [CrossRef]
    [Google Scholar]
  15. Lacy D. E., Smith A. W., Stableforth D. E., Smith G., Weller P. H., Brown M. R. 1995; Serum IgG response to Burkholderia cepacia outer membrane antigens in cystic fibrosis: assessment of cross-reactivity with Pseudomonas aeruginosa . FEMS Immunol Med Microbiol 10:253–261 [CrossRef]
    [Google Scholar]
  16. Mahenthiralingam E., Campbell M. E., Foster J., Lam J. S., Speert D. P. 1996; Random amplified polymorphic DNA typing of Pseudomonas aeruginosa isolates recovered from patients with cystic fibrosis. J Clin Microbiol 34:1129–1135
    [Google Scholar]
  17. Moore J. E., Xu J., Millar B. C., Crowe M., Elborn J. S. 2002; Improved molecular detection of Burkholderia cepacia genomovar III and Burkholderia multivorans directly from sputum of patients with cystic fibrosis. J Microbiol Methods 49:183–191 [CrossRef]
    [Google Scholar]
  18. National Committee for Clinical Laboratory Standards 2000 Performance Standard for Antimicrobial Susceptibility Testing , 5th edn. Approved standard M7-A6 Wayne, PA: National Committee for Clinical Laboratory Standards;
    [Google Scholar]
  19. Pitulle C., Citron D. M., Bochner B., Barbers R., Appleman M. D. 1999; Novel bacterium isolated from a lung transplant patient with cystic fibrosis. J Clin Microbiol 37:3851–3855
    [Google Scholar]
  20. Rajan S., Saiman L. 2002; Pulmonary infections in patients with cystic fibrosis. Semin Respir Infect 17:47–56 [CrossRef]
    [Google Scholar]
  21. Rogol M., Sechter I., Braunstein I., Gerichter C. B. 1983; Extended scheme for serotyping Campylobacter jejuni : results obtained in Israel from 1980 to 1981. J Clin Microbiol 18:283–286
    [Google Scholar]
  22. Towbin H., Staehelin T., Gordon J. 1992; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Biotechnology 24:145–149
    [Google Scholar]
  23. Wallet F., Perez T., Armand S., Wallaert B., Courcol R. J. 2002; Pneumonia due to Bordetella bronchiseptica in a cystic fibrosis patient: 16S rRNA sequencing for diagnosis confirmation. J Clin Microbiol 40:2300–2301 [CrossRef]
    [Google Scholar]
  24. Wellinghausen N., Essig A., Sommerburg O. 2005; Inquilinus limosus in patients with cystic fibrosis, Germany. Emerg Infect Dis 11:457–459 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.46466-0
Loading
/content/journal/jmm/10.1099/jmm.0.46466-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error