1887

Abstract

is a novel Gram-negative bacterium of the subdivision recently found in the airways of patients with cystic fibrosis (CF). Here, the authors report on the clinical courses of six CF patients colonized with . Five patients suffered from either an acute respiratory exacerbation or a progressive loss of pulmonary function, whereas one patient was in a stable clinical situation. This study focused on two aims: (i) the clonal analysis of isolates by random amplified polymorphic DNA (RAPD)-PCR, and (ii) the clarification of whether the presence of in the respiratory tract is associated with a specific serum antibody response. Serum IgG was detected by immunoblotting using whole-cell-lysate proteins as antigens. Sera from healthy blood donors (=10) and from CF patients colonized with (=10) were found to be immunoblot negative. All six -positive patients raised serum IgG antibodies against various antigens. Surprisingly, in one patient, a specific serum antibody response was already detected 1 year prior to -positive sputum cultures. Two prominent antigens were characterized by MALDI-MS: a 23 kDa protein revealed homology to the outer membrane lipoprotein OmlA of , and an 18 kDa protein to a protein-tyrosine phosphatase of . In conclusion, detection of is accompanied by a specific serum antibody response and may reflect the infectious/pathogenic potential of . Moreover, IgG immunoblotting may be useful to detect early infection with and may support the selective cultivation of this novel emerging pathogen.

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2006-10-01
2019-11-12
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