1887

Abstract

A rapid and inexpensive method for the detection of drug resistance in is essential for the effective control of tuberculosis. The aim of this study was to evaluate a colorimetric method using 2,3,5-triphenyltetrazolium chloride (TTC) for antibiotic susceptibility testing of isolates. Eleven multidrug-resistant (MDR) isolates of and 12 isolates which were susceptible to rifampicin (RIF) and isoniazid (INH) were used. The test was performed with a critical concentration of 0.2 μg ml for INH and 2.0 μg ml for RIF in 7H9GC broth with 0.625 μg TTC ml. Each isolate was inoculated under these conditions and inspected daily for colour changes; the results were obtained after a mean of 4.9 days. The sensitivity and specificity of this method were 100 % and 92 %, respectively, for both antibiotics. Considering the speed, technical ease and cost-effectiveness of this method, the TTC assay is a good alternative method for drug susceptibility testing of isolates.

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2006-12-01
2019-11-19
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