1887

Abstract

The genetic heterogeneity among and isolates obtained from apparently healthy cattle and sheep was investigated by random amplified polymorphic DNA (RAPD) analysis. A total of 348 isolates, consisting of (=218) and (=130), were analysed. All these isolates were successfully typed by RAPD analysis. The total numbers of band patterns defined by RAPD in cattle and sheep were 42 and 45, respectively. Of the 42 distinct types obtained from cattle, 37 types were observed in isolates (=115), and the remaining 5 were in isolates (=30). Of 45 distinct types obtained from sheep, 21 types were observed in isolates (=103), and 24 were in isolates (=100). It was concluded that a high degree of heterogeneity existed among the and isolates of healthy cattle and sheep.

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2006-03-01
2019-10-14
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References

  1. Açik, M. N. & Çetinkaya, B. ( 2005; ). The heterogeneity of Campylobacter jejuni and Campylobacter coli strains isolated from healthy cattle. Lett Appl Microbiol 41, 397–403.[CrossRef]
    [Google Scholar]
  2. Ertas, H. B., Çetinkaya, B., Muz, A. & Ongor, H. ( 2004; ). Genotyping of broiler-originated Campylobacter jejuni and Campylobacter coli isolates using fla typing and random amplified polymorphic DNA methods. Int J Food Microbiol 94, 203–209.[CrossRef]
    [Google Scholar]
  3. Hernandez, J., Fayos, A., Ferrus, M. A. & Owen, R. J. ( 1995; ). Random amplified polymorphic DNA fingerprinting of Campylobacter jejuni and Campylobacter coli isolated from human faeces, seawater and poultry products. Res Microbiol 146, 685–696.[CrossRef]
    [Google Scholar]
  4. Hernandez, J., Fayos, A., Alonso, J. L. & Owen, R. J. ( 1996; ). Ribotypes and AP-PCR fingerprints of thermophilic campylobacters from marine recreational waters. J Appl Bacteriol 80, 157–164.[CrossRef]
    [Google Scholar]
  5. Hilton, A. C., Mortiboy, D., Banks, J. G. & Penn, C. W. ( 1997; ). RAPD analysis of environmental, food and clinical isolates of Campylobacter spp. FEMS Immunol Med Microbiol 18, 119–124.[CrossRef]
    [Google Scholar]
  6. Madden, R. H., Moran, L. & Scates, P. ( 1996; ). Sub-typing of animal and human Campylobacter spp. using RAPD. Lett Appl Microbiol 23, 167–170.
    [Google Scholar]
  7. Mead, P. S., Slutsker, L., Dietz, V., McCaig, L. F., Bresee, J. S., Shapiro, C., Griffin, P. M. & Tauxe, R. V. ( 1999; ). Food-related illness and death in the United States. Emerg Infect Dis 5, 607–625.[CrossRef]
    [Google Scholar]
  8. Nadeau, E., Messier, S. & Quessy, S. ( 2002; ). Prevalence and comparison of genetic profiles of Campylobacter strains isolated from poultry and sporadic cases of campylobacteriosis in humans. J Food Prot 65, 73–78.
    [Google Scholar]
  9. Nielsen, E. M., Engberg, J., Fussing, V., Petersen, L., Brogren, C. H. & On, S. L. ( 2000; ). Evaluation of phenotypic and genotypic methods for subtyping Campylobacter jejuni isolates from humans, poultry, and cattle. J Clin Microbiol 38, 3800–3810.
    [Google Scholar]
  10. Park, R. W., Griffiths, P. L. & Moreno, G. S. ( 1991; ). Sources and survival of campylobacters: relevance to enteritis and the food industry. Soc Appl Bacteriol Symp Ser 20, 97–106.
    [Google Scholar]
  11. Scates, P., Moran, L. & Madden, R. H. ( 2003; ). Effect of incubation temperature on isolation of Campylobacter jejuni genotypes from foodstuffs enriched in Preston broth. Appl Environ Microbiol 69, 4658–4661.[CrossRef]
    [Google Scholar]
  12. Stanley, K. & Jones, K. ( 2003; ). Cattle and sheep farms as reservoirs of Campylobacter. J Appl Microbiol 94, 104–113.[CrossRef]
    [Google Scholar]
  13. Welsh, J. & McClelland, M. ( 1990; ). Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 18, 7213–7218.[CrossRef]
    [Google Scholar]
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