1887

Abstract

is a Gram-negative bacterium first identified in 1995. It can cause pertussis-like symptoms in humans. contains insertion sequences IS and IS, two frequently used targets in the PCR diagnosis of and infections. To investigate the prevalence of in Finnish and Dutch patients with pertussis-like symptoms and whether has caused any false-positive results in diagnostic PCRs, -specific real-time PCRs were developed. The Finnish methods were conventional IS PCR and -specific real-time PCR (LightCycler, Roche) targeting the gene. The Dutch methods were IS and IS PCRs with conventional or real-time formats and -specific real-time PCR targeting the homologue of IS. Of 11 319 nasopharyngeal swabs, 2804 were collected from Finnish patients from 2000 to 2003, and 8515 from Dutch patients from 1992 to 2003. DNA was not found in the samples analysed. The results suggest that is not among the causative agents of pertussis-like symptoms in Finnish and Dutch patients and thus does not in practice confound IS and IS PCRs.

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2006-08-01
2024-03-28
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References

  1. Altschul S. F., Madden T. L., Schaffer A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [CrossRef]
    [Google Scholar]
  2. Exner M. M., Lewinski M. A. 2002; Sensitivity of multiplex real-time PCR reactions, using the LightCycler and the ABI PRISM 7700 Sequence Detection System, is dependent on the concentration of the DNA polymerase. Mol Cell Probes 6:351–357
    [Google Scholar]
  3. Farrell D. J., Daggard G., Mukkur T. K. S. 1999; Nested duplex PCR to detect Bordetella pertussis and Bordetella parapertussis and its application in diagnosis of pertussis in nonmetropolitan southeast Queensland, Australia. J Clin Microbiol 37:606–610
    [Google Scholar]
  4. Favre D., Viret J. F. 1990; Nucleotide sequence of the recA gene of Bordetella pertussis . Nucleic Acids Res 18:4243 [CrossRef]
    [Google Scholar]
  5. Favre D., Cryz S. J. Jr, Viret J. F. 1991; Cloning of the recA gene of Bordetella pertussis and characterization of its product. Biochemie 73:235–244 [CrossRef]
    [Google Scholar]
  6. Fry N. K., Tzivra Y., Li T. & 7 other authors; 2004; Laboratory diagnosis of pertussis infections: the role of PCR and serology. J Med Microbiol 53:519–525 [CrossRef]
    [Google Scholar]
  7. Glare E. M., Paton J. C., Premier R. R., Lawrence A. J., Nisbet I. T. 1990; Analysis of a repetitive DNA sequence from Bordetella pertussis and its application to the diagnosis of pertussis using the polymerase chain reaction. J Clin Microbiol 28:1982–1987
    [Google Scholar]
  8. Gzyl A., Augustynowicz E., van Loo I., Slusarczyk J. 2001; Temporal nucleotide changes in pertactin and pertussis toxin genes in Bordetella pertussis strains isolated from clinical cases in Poland. Vaccine 20:299–303 [CrossRef]
    [Google Scholar]
  9. He Q., Mertsola J., Soini H., Skurnik M., Ruuskanen O., Viljanen M. K. 1993; Comparison of polymerase chain reaction with culture and enzyme immunoassay for diagnosis of pertussis. J Clin Microbiol 31:642–645
    [Google Scholar]
  10. He Q., Schmidt-Schläpfer G., Just M., Matter H., Nikkari S., Viljanen M. K., Mertsola J. 1996; Impact of polymerase chain reaction on clinical pertussis research: Finnish and Swiss experiences. J Infect Dis 174:1288–1295 [CrossRef]
    [Google Scholar]
  11. He Q., Viljanen M. K., Arvilommi H., Aittanen B., Mertsola J. 1998; Whooping cough caused by Bordetella pertussis and Bordetella parapertussis in an immunized population. JAMA (J Am Med Assoc) 280:635–637 [CrossRef]
    [Google Scholar]
  12. Heininger U., Stehr K., Schmitt-Grohe S., Lorenz C., Rost R., Christenson P. D., Uberall M., Cherry J. D. 1994; Clinical characteristics of illness caused by Bordetella parapertussis compared with illness caused by Bordetella pertussis . Pediatr Infect Dis J 13:306–309 [CrossRef]
    [Google Scholar]
  13. Heininger U., Schmidt-Schläpfer G., Cherry J. D., Stehr K. 2000; Clinical validation of a polymerase chain reaction assay for the diagnosis of pertussis by comparison with serology, culture, and symptoms during a large pertussis vaccine efficacy trial. Pediatrics 105:e31 [CrossRef]
    [Google Scholar]
  14. Jones C. E., Baumann U., Brown A. L. 2005; Automated methods of predicting the function of biological sequences go and blast. BMC Bioinformatics 6:272 http://www.biomedcentral.com/1471-2105/6/272 [CrossRef]
    [Google Scholar]
  15. Kerr J. R., Matthews R. C. 2000; Bordetella pertussis infection: pathogenesis, diagnosis, management, and the role of protective immunity. Eur J Clin Microbiol Infect Dis 19:77–78 [CrossRef]
    [Google Scholar]
  16. Ko K. S., Peck K. R., Oh W. S., Lee N. Y., Lee J. H., Song J. H. 2005; New species of Bordetella , Bordetella ansorpii sp. nov., isolated from the purulent exudate of an epidermal cyst. J Clin Microbiol 43:2516–2519 [CrossRef]
    [Google Scholar]
  17. Kuhl S. A. 1996; Promotion of bacteriophage induction and recombination by the cloned Bordetella pertussis recA gene is copy-number dependent. Curr Microbiol 32:162–167 [CrossRef]
    [Google Scholar]
  18. Lind-Brandberg L., Welinder-Olsson C., Lagergard T., Taranger J., Trollfors B., Zackrisson G. 1998; Evaluation of PCR for diagnosis of Bordetella pertussis and Bordetella parapertussis infections. J Clin Microbiol 36:679–683
    [Google Scholar]
  19. Lindquist S. W., Weber D. J., Mangum M. E., Hollis D. G., Jordan J. 1995; Bordetella holmesii sepsis in an asplenic adolescent. Pediatr Infect Dis J 14:813–815 [CrossRef]
    [Google Scholar]
  20. Loeffelholz M. J., Thompson C. J., Long K. S., Gilchrist M. J. 1999; Comparison of PCR, culture, and direct fluorescent-antibody testing for detection of Bordetella pertussis . J Clin Microbiol 37:2872–2876
    [Google Scholar]
  21. Mazengia E., Silva E. A., Peppe J. A., Timperi R., George H. 2000; Recovery of Bordetella holmesii from patients with pertussis-like symptoms: use of pulsed-field gel electrophoresis to characterize circulating strains. J Clin Microbiol 38:2330–2333
    [Google Scholar]
  22. Mooi F., van Oirschot H., Heuvelman K., van der Heide H. G., Gaastra W., Willems R. J. 1998; Polymorphism in the Bordetella pertussis virulence factors P.69/pertactin and pertussis toxin in The Netherlands: temporal trends and evidence for vaccine-driven evolution. Infect Immun 66:670–675
    [Google Scholar]
  23. Mooi F., He Q., van Oirschot H., Mertsola J. 1999; Variation in the Bordetella pertussis virulence factors pertussis toxin and pertactin in vaccine strains and clinical isolates in Finland. Infect Immun 67:3133–3134
    [Google Scholar]
  24. Morris J. T., Myers M. 1998; Bacteremia due to Bordetella holmesii . Clin Infect Dis 27:912–913 [CrossRef]
    [Google Scholar]
  25. Muller F. M., Hoppe J. E., Wirsing von Konig C. H. 1997; Laboratory diagnosis of pertussis: state of art in 1997. J Clin Microbiol 35:2435–2443
    [Google Scholar]
  26. Niesters H. G. 2001; Quantitation of viral load using real-time amplification techniques. Methods 25:419–429 [CrossRef]
    [Google Scholar]
  27. Parkhill J., Sebaihia M., Preston A. & 50 other authors; 2003; Comparative analysis of the genome sequences of Bordetella pertussis , Bordetella parapertussis and Bordetella bronchiseptica . Nat Genet 35:32–40 [CrossRef]
    [Google Scholar]
  28. Pietilä J., He Q., Oksi J., Viljanen M. K. 2000; Rapid differentiation of Borrelia garinii from Borrelia afzelii and Borrelia burgdorferi sensu stricto by LightCycler fluorescence melting curve analysis of a PCR product of the recA gene. J Clin Microbiol 38:2756–2759
    [Google Scholar]
  29. Poddar S. K. 2003; Detection and discrimination of B. pertussis and B. holmesii by real-time PCR targeting IS 481 using a beacon probe and probe-target melting analysis. Mol Cell Probes 17:91–98 [CrossRef]
    [Google Scholar]
  30. Reischl U., Lehn N., Sanden G. N., Loeffelholz M. J. 2001; Real-time PCR assay targeting IS 481 of Bordetella pertussis and molecular basis for detecting Bordetella holmesii . J Clin Microbiol 39:1963–1966 [CrossRef]
    [Google Scholar]
  31. Ririe K. M., Rasmussen R. P., Wittwer C. T. 1997; Product differentiation by analysis of DNA melting curves during the polymerase chain reaction. Anal Biochem 245:154–160 [CrossRef]
    [Google Scholar]
  32. Russell F. M., Davis J. M., Whipp M. J., Janssen P. H., Ward P. B., Vyas J. R., Starr M., Sawyer S. M., Curtis N. 2001; Severe Bordetella holmesii infection in a previously healthy adolescent confirmed by gene sequence analysis. Clin Infect Dis 33:129–130 [CrossRef]
    [Google Scholar]
  33. Shepard C. W., Daneshvar M. I., Kaiser R. M. & 7 other authors; 2004; Bordetella holmesii bacteremia: a newly recognized clinical entity among asplenic patients. Clin Infect Dis 38:799–804 [CrossRef]
    [Google Scholar]
  34. Tang Y. W., Hopkins M. K., Kolbert C. P., Hartley P. A., Severance P. J., Persing D. H. 1998; Bordetella holmesii -like organisms associated with septicemia, endocarditis, and respiratory failure. Clin Infect Dis 26:389–392 [CrossRef]
    [Google Scholar]
  35. Templeton K. E., Scheltinga S. A., van der Zee A., Diederen B. M., van Kruijssen A. M., Goossens H., Kuijper E., Claas E. C. J. 2003; Evaluation of real-time PCR for detection of and discrimination between Bordetella pertussis , Bordetella parapertussis , and Bordetella holmesii for clinical diagnosis. J Clin Microbiol 41:4121–4126 [CrossRef]
    [Google Scholar]
  36. Teo I. A., Choi J. W., Morlese J., Taylor G., Shaunak S. 2002; LightCycler qPCR optimisation for low copy number target DNA. J Immunol Methods 270:119–133 [CrossRef]
    [Google Scholar]
  37. van der Zee A., Agtenberg C., van Agterveld M., Peeters M., Mooi F. 1993a; Characterization of IS 1001 , an insertion sequence element of Bordetella parapertussis . J Bacteriol 175:141–147
    [Google Scholar]
  38. van der Zee A., Agtenberg C., Peeters M., Schellekens J., Mooi F. 1993b; Polymerase chain reaction assay for pertussis: simultaneous detection and discrimination of Bordetella pertussis and Bordetella parapertussis . J Clin Microbiol 31:2134–2140
    [Google Scholar]
  39. van der Zee A., Agterberg C., Peeters M., Mooi F., Schellekens J. 1996; A clinical validation of Bordetella pertussis and Bordetella parapertussis polymerase chain reaction: comparison with culture and serology using samples from patients with suspected whooping cough from a highly immunized population. J Infect Dis 174:89–96 [CrossRef]
    [Google Scholar]
  40. van der Zee A., Mooi F., Van Embden J., Musser J. 1997; Molecular evolution and host adaptation of Bordetella spp. phylogenetic analysis using multilocus enzyme electrophoresis and typing with three insertion sequences. J Bacteriol 179:6609–6617
    [Google Scholar]
  41. Vielemayer O., Crouch J. Y., Edberg S. C., Howe J. G. 2004; Identification of Bordetella pertussis in a critically ill human immunodefiency virus-infected patient by direct genotypical analysis of Gram-stained material and discrimination from B. holmesii by using a unique recA gene restriction enzyme site. J Clin Microbiol 42:847–849 [CrossRef]
    [Google Scholar]
  42. Wang J., Yang Y., Li J., Mertsola J., Arvilommi H., Shen X., He Q. 2002; Infantile pertussis rediscovered in China. Emerg Infect Dis 8:859–861 [CrossRef]
    [Google Scholar]
  43. Weyant R. S., Hollis D. G., Weaver R. E. & 7 other authors; 1995; Bordetella holmesii sp. nov., a new Gram-negative species associated with septicemia. J Clin Microbiol 33:1–7
    [Google Scholar]
  44. Yih W. K., Silva E. A., Ida J., Harrington N., Lett S. M., George H. 1999; Bordetella holmesii -like organisms isolated from Massachusetts patients with pertussis-like symptoms. Emerg Infect Dis 5:441–443 [CrossRef]
    [Google Scholar]
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