1887

Abstract

is a Gram-negative bacterium first identified in 1995. It can cause pertussis-like symptoms in humans. contains insertion sequences IS and IS, two frequently used targets in the PCR diagnosis of and infections. To investigate the prevalence of in Finnish and Dutch patients with pertussis-like symptoms and whether has caused any false-positive results in diagnostic PCRs, -specific real-time PCRs were developed. The Finnish methods were conventional IS PCR and -specific real-time PCR (LightCycler, Roche) targeting the gene. The Dutch methods were IS and IS PCRs with conventional or real-time formats and -specific real-time PCR targeting the homologue of IS. Of 11 319 nasopharyngeal swabs, 2804 were collected from Finnish patients from 2000 to 2003, and 8515 from Dutch patients from 1992 to 2003. DNA was not found in the samples analysed. The results suggest that is not among the causative agents of pertussis-like symptoms in Finnish and Dutch patients and thus does not in practice confound IS and IS PCRs.

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2006-08-01
2019-10-14
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