1887

Abstract

In this study, the ease of selection of clarithromycin resistance was investigated in clarithromycin-susceptible strains isolated from patients with infection prior to the administration of triple-combination eradication therapy (clarithromycin plus amoxicillin plus a proton pump inhibitor). Clarithromycin-susceptible strains isolated from ten patients in whom the eradication therapy was successful and from six patients in whom the eradication therapy was unsuccessful were exposed serially to subinhibitory concentrations of clarithromycin. The number of transfers required for the MICs of the strains to increase by 8- and 32-fold were 6.6 and 7.2, respectively, in the successful eradication group, and as few as 2.4 and 1.5, respectively, in the unsuccessful eradication group. The number of transfers required for the A2142G or A2143G point mutation of the 23S rRNA gene to be detected in the strains were 5 and 8, respectively, for the strains in the successful eradication group, and 1 and 2, respectively, for the strains in the unsuccessful eradication group. These results suggest that patients in the unsuccessful eradication group were infected with strains of that readily became resistant to clarithromycin on exposure to the drug.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.46316-0
2006-06-01
2019-10-18
Loading full text...

Full text loading...

/deliver/fulltext/jmm/55/6/737.html?itemId=/content/journal/jmm/10.1099/jmm.0.46316-0&mimeType=html&fmt=ahah

References

  1. Broutet, N., Marais, A., Lamouliatte, H., de Mascarel, A., Samoyeau, R., Salamon, R. & Mégraud, F. ( 2001; ). cagA status and eradication treatment outcome of anti-Helicobacter pylori triple therapies in patients with nonulcer dyspepsia. J Clin Microbiol 39, 1319–1322.[CrossRef]
    [Google Scholar]
  2. Ducóns, J. A., Santolaria, S., Guirao, R., Ferrero, M., Montoro, M. & Gomollón, F. ( 1999; ). Impact of clarithromycin resistance on the effectiveness of a regimen for Helicobacter pylori: a prospective study of 1-week lansoprazole, amoxicillin and clarithromycin in active peptic ulcer. Aliment Pharmacol Ther 13, 775–780.[CrossRef]
    [Google Scholar]
  3. European Helicobacter pylori Study Group ( 1997; ). Current European concepts in the management of Helicobacter pylori infection. The Maastricht Consensus Report. Gut 41, 8–13.[CrossRef]
    [Google Scholar]
  4. Fontana, C., Favaro, M., Minelli, S., Criscuolo, A. A., Pietroiusti, A., Galante, A. & Favalli, C. ( 2002; ). New site of modification of 23S rRNA associated with clarithromycin resistance of Helicobacter pylori clinical isolates. Antimicrob Agents Chemother 46, 3765–3769.[CrossRef]
    [Google Scholar]
  5. Haas, C. E., Nix, D. E. & Schentag, J. J. ( 1990; ). In vitro selection of resistant Helicobacter pylori. Antimicrob Agents Chemother 34, 1637–1641.[CrossRef]
    [Google Scholar]
  6. Japanese Society for Helicobacter Research ( 2000; ). Guidelines for diagnosis and treatment of Helicobacter pylori infection. Jpn J Helicobacter Res 2, 2–12 (in Japanese).
    [Google Scholar]
  7. Kim, J. J., Kim, J. G. & Kwon, D. H. ( 2003; ). Mixed-infection of antibiotic susceptible and resistant Helicobacter pylori isolates in a single patients and underestimation of antimicrobial susceptibility testing. Helicobacter 8, 202–206.[CrossRef]
    [Google Scholar]
  8. Kobayashi, I., Toda, H., Hasegawa, M., Nishida, M., Fujioka, T. & Nasu, M. ( 1996; ). Drug susceptibility of Helicobacter pylori freshly isolated from gastrointestinal mucosa of patients with peptic ulcer. Jpn J Chemother 14, 719–722 (in Japanese with English summary).
    [Google Scholar]
  9. Kobayashi, I., Saika, T., Muraoka, H., Inoue, M. & Nasu, M. ( 2001; ). Relationship between clarithromycin breakpoint for Helicobacter pylori and point mutation in 23S rRNA gene. Jpn J Chemother 46, 236–240 (in Japanese with English summary).
    [Google Scholar]
  10. Murakami, K., Sato, R., Okimoto, T., Nasu, M., Fujioka, T., Kodama, M., Kagawa, T., Sato, S., Abe, H. & Arita, T. ( 2002; ). Eradication rates of clarithromycin-resistant Helicobacter pylori using either rabeprazole or lansoprazole plus amoxicillin and clarithromycin. Aliment Pharmacol Ther 16, 1933–1938.[CrossRef]
    [Google Scholar]
  11. NCCLS ( 2003; ). Performance standards for antimicrobial susceptibility testing. Thirteenth informational supplement M100-S13. Wayne, PA: NCCLS.
  12. Suzuki, J., Muraoka, H., Kobayashi, I., Fujita, T. & Mine, T. ( 1999; ). Rare incidence of interspousal transmission of Helicobacter pylori in asymptomatic individuals in Japan. J Clin Microbiol 37, 4174–4176.
    [Google Scholar]
  13. Tait-Kamradt, A., Davies, T., Cronan, M., Jacobs, M. R., Appelbaum, P. C. & Sutcliffe, J. ( 2000; ). Mutations in 23S rRNA and ribosomal protein L4 account for resistance in pneumococcal strains selected in vitro by macrolide passage. Antimicrob Agents Chemother 44, 2118–2125.[CrossRef]
    [Google Scholar]
  14. Taylor, D. E., Ge, Z., Purych, D., Lo, T. & Hiratsuka, K. ( 1997; ). Cloning and sequence analysis of two copies of a 23S rRNA gene from Helicobacter pylori and association of clarithromycin resistance with 23S rRNA mutations. Antimicrob Agents Chemother 41, 2621–2628.
    [Google Scholar]
  15. van den Hulst, R. W., Keller, J. J., Rauws, E. A. & Tytgat, G. N. ( 1996; ). Treatment of Helicobacter pylori infection: a review of the world literature. Helicobacter 1, 6–19.[CrossRef]
    [Google Scholar]
  16. Versalovic, J., Osato, M. S., Spakovsky, K. & 7 other authors ( 1997; ). Point mutations in the 23S rRNA gene of Helicobacter pylori associated with different levels of clarithromycin resistance. J Antimicrob Chemother 40, 283–286.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.46316-0
Loading
/content/journal/jmm/10.1099/jmm.0.46316-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error