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Abstract

The C protein (Bac) of (group B streptococcus; GBS) is an IgA binding protein encoded by , of which at least 39 sequence types have been described, based on polymorphisms in the repeated region of the cell-wall-spanning domain (‘ sequence types’). C is usually found in serotype Ib, less commonly in serotype II, and rarely in other serotypes. The aim of this study was to examine the prevalence, variety and distribution, among GBS serotypes and between invasive and superficial isolates, of sequence types. A total of 1101 GBS isolates were tested, from 10 countries, with a specific PCR, and amplicons from all 255 (23 %) with positive results were sequenced. Ninety-seven percent (184/190) of serotype Ib and 37 % of serotype II isolates were positive. The C protein gene () was present in 98 % (251/255), and insertion sequences IS and IS in 94 % (239/255), of -positive isolates. The authors identified 59 sequence types belonging to 19 groups, based on length, from 496 to 946 bp, with up to six sequence variants (a–f) in each group. The median sequence length of invasive isolates was significantly shorter than that of superficial isolates overall (640 versus 586 bp; <0.001) and specifically for serotype Ib (541 versus 676 bp; <0.001), and invasive isolates were significantly ( <0.001) more likely to have one or more 18 bp deletions relative to the original published sequence (X59771). sequence typing is a useful addition to the previously described genotyping system, and will help to predict relative virulence among serotype Ib strains.

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2006-07-01
2019-10-19
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vol. , part 7, pp. 829–837

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