1887

Abstract

This study was carried out in order to compare two PCR-based methods in the detection of . The first PCR method was based on primers for the 16S rRNA gene and the second method was based on specific primers that targeted the glucosyltransferase gene (). Each PCR was performed with eight different streptococci from the viridans group, five other streptococci and 17 different non-streptococcal bacterial strains. Direct use of the 16S rRNA gene-specific primers revealed that and were also detected. After amplifying the 16S rRNA gene with universal primers and subsequently performing nested PCR, the -specific nested primers based on the 16S rRNA gene detected all tested streptococci. There was no cross-reaction of the primers after direct PCR. Our results indicate that direct PCR and nested PCR based on 16S rRNA genes can reveal false-positive results for oral streptococci and lead to an overestimation of the prevalence of with regards to its role as the most prevalent causative agent of dental caries.

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2006-01-01
2019-10-15
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