RT Journal Article SR Electronic(1) A1 Soto, Sara M. A1 Rodríguez, Irene A1 Rodicio, M. Rosario A1 Vila, Jordi A1 Mendoza, M. CarmenYR 2006 T1 Detection of virulence determinants in clinical strains of Salmonella enterica serovar Enteritidis and mapping on macrorestriction profiles JF Journal of Medical Microbiology, VO 55 IS 4 SP 365 OP 373 DO https://doi.org/10.1099/jmm.0.46257-0 PB Microbiology Society, SN 1473-5644, AB A total of 80 strains of Salmonella enterica serovar Enteritidis, causing gastroenteritis (G) or bacteraemia (B), and three control strains (C), were subjected to: (i) detection of 14 chromosomally and 1 plasmid-located virulence genes by PCR, (ii) detection of DNA polymorphisms by XbaI and BlnI PFGE, and cluster analysis, (iii) mapping of the 15 screened sequences on macrorestriction profiles and (iv) comparison of the screening and mapping results with data available for other Salmonella strains. Identical virulence genotypes and very similar macrorestriction profiles were shown by most S. Enteritidis strains. However, a number of B strains belonged to genomic types with polymorphisms affecting fragments carrying (SPI2-slyA), (SPI2-slyA-phoP/Q-agfA), (SPI4 and/or stn) and spvC. The information obtained provides the basis for further studies on the genetic background of virulence and the molecular epidemiology of S. Enteritidis., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.46257-0