1887

Journal of Medical Microbiology logo

Volume 55, Issue 5

and isolated from human clinical specimens Free

Abstract

A polyphasic identification approach was applied to a group of 11 novobiocin-resistant staphylococci isolated from human clinical materials. Phenotypic characteristics obtained by both commercial and conventional tests assigned eight strains as and three strains as ambiguous . In contrast to biotyping, ribotyping with RI and dIII restriction endonucleases and whole-cell protein fingerprinting assigned six analysed strains as , and five strains as . Confirmation of the identification was done by partial 16S rRNA gene sequencing and isolates were verified by a PCR assay targeting the gene. From the data it has been implied that ribotyping and whole-cell protein analysis can be used to differentiate between the biochemically almost indistinguishable species , and . The present study confirms what is believed to be the first occurrence of in a relevant human clinical material in the Czech Republic and describes what is believed to be the first-ever isolation of from human clinical material.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): CCM, Czech Collection of Microorganisms and CoNS, coagulase-negative staphylococci
SGM
Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.46246-0
2006-05-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/55/5/523.html?itemId=/content/journal/jmm/10.1099/jmm.0.46246-0&mimeType=html&fmt=ahah

References

  1. Alcaráz L. E., Satorres S. E., Lucero R. M., Puig de Centorbi O. N. 2003; Species identification, slime production and oxacillin susceptibility in coagulase-negative staphylococci isolated from nosocomial specimens. Braz J Microbiol 34:45–51
     [Google Scholar]
  2. Becker K., Harmsen D., Mellmann A., Meier C., Schumann P., Peters G., von Eiff C. 2004; Development and evaluation of a quality-controlled ribosomal sequence database for 16S ribosomal DNA-based identification of Staphylococcus species. J Clin Microbiol 42:4988–4995 [CrossRef]
     [Google Scholar]
  3. Blaiotta G., Ercolini D., Mauriello G., Salzano G., Villani F. 2004; Rapid and reliable identification of Staphylococcus equorum by a species-specific PCR assay targeting the sod A gene. Syst Appl Microbiol 27:696–702 [CrossRef]
     [Google Scholar]
  4. Bockelmann W., Hoppe-Seyler T. S. 2001; The surface flora of bacterial smear-ripened cheese from cow's and goat's milk. Int Dairy J 11:307–314 [CrossRef]
     [Google Scholar]
  5. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805 [CrossRef]
     [Google Scholar]
  6. Devriese L. A., Poutrel B., Kilpper-Bälz R., Schleifer K. H. 1983; Staphylococcus gallinarum and Staphylococcus caprae , two new species from animals. Int J Syst Bacteriol 33:480–486 [CrossRef]
     [Google Scholar]
  7. Hauschild T. 2001; Phenotypic and genotypic identification of staphylococci isolated from wild small mammals. Syst Appl Microbiol 24:411–416 [CrossRef]
     [Google Scholar]
  8. Izard N. C., Hächler H., Grehn M., Kayser F. H. 1992; Ribotyping of coagulase-negative staphylococci with special emphasis on intraspecific typing of Staphylococcus epidermidis . J Clin Microbiol 30:817–823
     [Google Scholar]
  9. Lambert L. H., Cox, T., Mitchell K., Rosselló-Mora R. A., Del Cueto C., Dodge D. E., Orkand P. ., Cano R. J. 1998; Staphylococcus succinus sp. nov., isolated from Dominican amber. Int J Syst Bacteriol 48:511–518 [CrossRef]
     [Google Scholar]
  10. Mannerová S., Pantůček R., Doškař J., Švec P., Snauwaert C., Vancanneyt M., Swings J., Sedláček I. 2003; Macrococcus brunensis sp. nov., Macrococcus hajekii sp. nov.and Macrococcus lamae sp. nov., from the skin of llamas. Int J Syst Evol Microbiol 53:1647–1654 [CrossRef]
     [Google Scholar]
  11. Marsou R., Bes M., Brun Y., Boudouma M., Idrissi L., Meugnier H., Freney J., Etienne J. 2001; Molecular techniques open up new vistas for typing of coagulase-negative staphylococci. Pathol Biol 49:205–215 [CrossRef]
     [Google Scholar]
  12. Meugnier H., Bes M., Vernozy-Rozand C., Mazuy C., Brun Y., Freney J., Fleurette J. 1996; Identification and ribotyping of Staphylococcus xylosus and Staphylococcus equorum strains isolated from goat milk and cheese. Int J Food Microbiol 31:325–331 [CrossRef]
     [Google Scholar]
  13. Nagase N., Sasaki A., Yamashita K., Shimizu A., Wakita Y., Kitai S., Kawano J. 2001; Isolation and species distribution of staphylococci from animal and human skin. J Vet Med Sci 64:245–250
     [Google Scholar]
  14. Pennington T. H., Harker C., Thomson-Carter F. 1991; Identification of coagulase-negative staphylococci by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and rRNA restriction patterns. J Clin Microbiol 29:390–392
     [Google Scholar]
  15. Pinna A., Zanetti S., Sotgiu M., Sechi L. A., Fadda G., Carta F. 1999; Identification and antibiotic susceptibility of coagulase negative staphylococci isolated in corneal/external infections. Br J Ophthalmol 83:771–773 [CrossRef]
     [Google Scholar]
  16. Place R. B., Hiestand D., Burri S., Teuber M. 2002; Staphylococcus succinus subsp. casei subsp. nov., a dominant isolate from a surface ripened cheese. Syst Appl Microbiol 25:353–359 [CrossRef]
     [Google Scholar]
  17. Place R. B., Hiestand D., Gallmann H. R., Teuber M. 2003; Staphylococcus equorum subsp. linens , subsp. nov., a starter culture component for surface ripened semi-hard cheeses. Syst Appl Microbiol 26:30–37 [CrossRef]
     [Google Scholar]
  18. Pot B., Vandamme P., Kersters K. 1994; Analysis of electrophoretic whole-organism protein fingerprints. In Chemical Methods in Prokaryotic Systematics pp  493–521 Edited by Goodfellow M., O'Donnell A. G. Chichester: Wiley;
     [Google Scholar]
  19. Rantsiou K., Iacumin L., Cantoni C., Comi G., Cocolin L. 2005; Ecology and characterization by molecular methods of Staphylococcus species isolated from fresh sausages. Int J Food Microbiol 97:277–284 [CrossRef]
     [Google Scholar]
  20. Schleifer K. H., Kilpper-Bälz R., Devriese L. A. 1984; Staphylococcus arlettae sp. nov., S. equorum sp. nov. and S. kloosii sp. nov. Three new coagulase-negative, novobiocin-resistant species from animal. Syst Appl Microbiol 5:501–509 [CrossRef]
     [Google Scholar]
  21. Siqueira J. F. Jr, Lima K. C. 2002; Staphylococcus epidermidis and Staphylococcus xylosus in a secondary root canal infection with persistent symptoms: a case report. Aust Endod J 28:61–63 [CrossRef]
     [Google Scholar]
  22. Spergser J., Wieser M., Taubel M., Rossello-Mora R. A., Rosengarten R., Busse H. J. 2003; Staphylococcus nepalensis sp. nov., isolated from goats of the Himalayan region. Int J Syst Evol Microbiol 53:2007–2011 [CrossRef]
     [Google Scholar]
  23. Švec P., Sedláček I., Pantůček R., Devriese L. A., Doškař J. 2001; Evaluation of ribotyping for characterization and identification of Enterococcus haemoperoxidus and Enterococcus moraviensis strains. FEMS Microbiol Lett 203:23–27 [CrossRef]
     [Google Scholar]
  24. Švec P., Vancanneyt M., Sedláček I., Engelbeen K., Štetina V., Swings J., Petráš P. 2004; Reclassification of Staphylococcus pulvereri Zakrzewska-Czerwińska et al. , 1995 as a later synonym of Staphylococcus vitulinus Webster et al. , 1994. Int J Syst Evol Microbiol 54:2213–2215 [CrossRef]
     [Google Scholar]
  25. Takahashi T., Satoh I., Kikuchi N. 1999; Phylogenetic relationships of 38 taxa of the genus Staphylococcus based on 16S rRNA gene sequence analysis. Int J Syst Bacteriol 49:725–728 [CrossRef]
     [Google Scholar]
  26. Vernozy-Rozand C., Mazuy C., Meugnier H., Bes M., Lasne Y., Fiedler F., Etienne J., Freney J. 2000; Staphylococcus fleurettii sp. nov., isolated from goat's milk cheeses. Int J Syst Bacteriol 50:1521–1527 [CrossRef]
     [Google Scholar]
  27. Villard L., Kodjo A., Borges E., Maurin F., Richard Y. 2000; Ribotyping and rapid identification of Staphylococcus xylosus by 16–23S spacer amplification. FEMS Microbiol Lett 185:83–87
     [Google Scholar]
  28. von Eiff C., Proctor R. A., Peters G. 2001; Coagulase-negative staphylococci. Pathogens have major role in nosocomial infections. Postgrad Med 110:63–76
     [Google Scholar]
  29. Woods G. L., Washington J. A. 1995; Antibacterial susceptibility tests: dilution and disk-diffusion methods. In Manual of Clinical Microbiology , 6th edn. pp  1327–1341 Edited by Murray P. R., Baron E. J., Pfaller M. A., Tenover F. C., Yolken R. H. Washington, DC: American Society for Microbiology;
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.46246-0
Loading
Staphylococcus equorum and Staphylococcus succinus isolated from human clinical specimens
J. Med. Microbiol. 55, 523 (2006); https://doi.org/10.1099/jmm.0.46246-0
/content/journal/jmm/10.1099/jmm.0.46246-0
/content/journal/jmm/10.1099/jmm.0.46246-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error