1887

Abstract

A polyphasic identification approach was applied to a group of 11 novobiocin-resistant staphylococci isolated from human clinical materials. Phenotypic characteristics obtained by both commercial and conventional tests assigned eight strains as and three strains as ambiguous . In contrast to biotyping, ribotyping with RI and dIII restriction endonucleases and whole-cell protein fingerprinting assigned six analysed strains as , and five strains as . Confirmation of the identification was done by partial 16S rRNA gene sequencing and isolates were verified by a PCR assay targeting the gene. From the data it has been implied that ribotyping and whole-cell protein analysis can be used to differentiate between the biochemically almost indistinguishable species , and . The present study confirms what is believed to be the first occurrence of in a relevant human clinical material in the Czech Republic and describes what is believed to be the first-ever isolation of from human clinical material.

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2006-05-01
2020-04-10
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